適堿性細菌 的英文怎麼說
中文拼音 [shìjiǎnxìngxìjūn]
適堿性細菌
英文
basophilous bacterium- 適 : 形容詞1 （適合） fit; suitable; proper 2 （恰好） right; opportune 3 （舒服） comfortable; well Ⅱ...
- 堿 : 名詞1. （含氫氧根的化合物的統稱） alkali2. （碳酸鈉） soda
- 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
- 細 : 形容詞1 （條狀物橫剖面小） thin; slender 2 （顆粒小） in small particles; fine 3 （音量小） thin ...
- 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
- 細菌 : germ; bacterium （pl. bacteria）; fungus （pl. fungi）
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Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments. the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose, sucrose, malic acid, mannitol ), propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum, at a ph range of 4 to 8. 5, at a saline concentration range of 0. 01 % to 1. 5 % ; low nlv " concentration had little effect on its nitrogenase activity. ara could also be detected when it grow in the culture media with 5mmol / l ntv "
W12菌株對環境因子的適應性研究：無氮培養條件下，測定溫度、碳源、酸堿度、滲透壓對w12生長及固氮能力的影響，結果表明，在15 - 40下均能生長並表達固氮酶活性，其最適生長及固氮的溫度為25 - 35 ；能利用葡萄糖、蔗糖、蘋果酸、甘露醇等多種碳源生長並固氮，當培養基中同時存在蔗糖和蘋果酸時，細菌生長和固氮活性最強；在偏酸和偏堿的條件下（ ph4 . 5 - 8 . 5 ）均能保持較強的生長勢和較高的固氮酶活性，並能通過調節自身代謝平衡並適應環境的酸、堿性變化，使培養液趨于中性：能耐受較高的滲透壓，培養液中卜、 5 naci濃度對其生長和固氮酶活性影響不大，當naci濃度升至2時，菌株的生長勢及固氮酶活性才有所下降：低濃度的鉸對其固氮酶活性影響不大，在0So some methods suitable to large plasmid extraction, including lysis by sds and a method from a literature, were used to try to extract the large plasmid from the strain cell. the lysis reactions in these two methods are gentle, so the large plasmid cannot be injured in the lysis process, opposite to lysis by alkali. it would be helpful to keep the integrality of the large plasmid during the extraction
因此我們採用了適合於大質粒提取的sds法，和文獻中應用於硝基苯降解性質粒的提取方法，來嘗試對菌株細胞進行質粒提取，這兩種方法裂解反應溫和，不會像堿裂解法那樣，在裂解過程中損壞質粒，可以實現質粒提取的完整性。
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