選出物 的英文怎麼說
中文拼音 [xuǎnchūwù]
選出物
英文
cull-
In the research field of food preservation, an intense interest for scientists is focused on the isolation of antimicrobial substances from microbial metabolites. natural biopreservatives derived from traditional fermented foods are generally recognized as safe. in this paper, one strain screened for its safety and broad antimicrobial spectrum and its antimicrobial substance - - bacteriocin are studied in detail
本文從傳統的發酵食品中篩選出一株安全、具有廣譜抗菌活性的芽孢桿菌,並對其抗菌活性物質細菌素進行分析,為微生物天然生物防腐劑的開發以及在食品中的應用提供理論依據。After careful studying their relative importance to immune response and the possibility of the match, seventeen sequences of interest were selected for further experiment, including estss analogous to 11. 5kd antibacterial peptide, lysozyme, serine protease and its inhibitor, lectin, antifreeze protein, et al. primers designed according to the sequences were used to amplify the corresponding estss from both blood and cephalothorax cdna library
在仔細分析了它們在免疫系統中的重要性和在對蝦中出現的可能性之後,從中選出了17條可能編碼抗菌肽,溶菌酶,凝集素、絲氨酸蛋白酶及其抑制劑,抗凍蛋白等蛋白質的序列,以此為依據設計引物,在中國對蝦的血液和頭胸部cdna文庫中擴增相應的序列。With the selected outgroup - chlamys farreri, the phylogenetic trees suggested that zhejiang population has differentiated. 3. in pb - pa and y1 - p0 families of argopecten irradians, 12 and 10 rapd primers were selected, which
在海灣扇貝pb - pa與y1 - p0家系中,分別篩選出12和10條引物,其擴增出的產物在雙親中呈現多態的片段。Since 1927 that annual designation has gone to the news maker who has most influenced the year's events.
自1927年以來,每年都選出對當年影響最大的新聞人物。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中,轉化大腸桿菌dh5菌株,篩選氨芐青霉素抗性菌落,提取質粒經酶切鑒定、 pcr分析以及確證性測序證明,所克隆的1500bp左右的片段含有完整的3abc基因,與國外參考序列相比,同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后,亞克隆3abc基因至原核表達載體ptriex - 4neo中,通過酶切鑒定、 pcr擴增以及序列分析,發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失,碰巧在3ab基因后形成一終止密碼子,但3ab基因的閱讀框架完整,選出含有3ab基因完整閱讀框架的陽性克隆,用iptg誘導表達,收集菌液進行sds - page電泳、 westernblotting分析,結果表明, 3ab基因在大腸桿菌中成功表達,其表達產物為分子量33 . 5ku的融合蛋白,並能被口蹄疫病毒陽性血清識別。經薄層掃描分析,表達量占總蛋白量的26以上。Abstract : selective mechanisms exist in organism and biosphere, which select the cells with less dna injuy and reproduces them. there are two models ; mechanisms 1 and mechanisms 2, represented respectively b bacteria and mechanolytes of human beings. cells with mechanisms 1 ( e. g. bacteria, generative cells, cancer cells ) can reproduce infinitely, while somatic cells can not. there are deep - going reasons behind the two categories of selective mechanisms. the canceration of the cells is a degenerating process from mechanisms 2 to mechanisms 1
摘要生物界存在一類篩選機制,它將dna損傷較小的細胞篩選出來,產生後代.主要有兩類模式機制1和機制2 ,分別以細菌和人的成纖維細胞為代表,那些具有篩選機制1的細胞(如:細菌、生殖細胞、癌細胞)能無限傳代,而體細胞則不能.這兩類篩選機制背後有更深刻的原因,而細胞的癌變正是從機制2退化為機制1的過程。This paper applies kami ' s method to analyze conservative scheme in lagrangian coordinate, and then selects one of upwind schemes which do not show oscillations, finally applies it to construct high resolution scheme. numerical flux of one order scheme is used near interface, while numerical flux of high resolution scheme is adopted in other area
本文在對lagrange坐標系下的守恆型格式進行詳細分析后,甄選出不震蕩的一階迎風型格式,然後應用於lagrange坐標系下的高解析度格式,在物質交界面處採用不震蕩的一階迎風型格式的數值通量,而在其餘地方採用高解析度格式的數值通量。And l. mollis hemsl, a random amplified polymorphic dna ( rapd ) analysis was performed for the first time. the screening of 40 decamer oligonucleotides allowed the selection of 11 primers used for the analysis, the dna fingerprints of 10 samples were constituted and the genetics evidence was furnished. to study on the contents and chemical constituents of essential oil from leaves and fruits of different species of litsea lam, the chemical compositions were separated and identified by gc / ms
為了評價山雞椒和毛葉木姜子的不同品種和品系,採用rapd技術,從40個引物中篩選出11個特徵引物,首次獲得山雞椒和毛葉木姜子以及它們的栽培品系的dna分子指紋圖譜,從分子水平上較全面地評價了各樣品間的親緣關系,從而為栽培品種的劃分提供了一定的遺傳學上的證據。Using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, tow different fragments of sox gene, essox3 and essox22 were amplified from female and male eriocheir sinensis, the sequence results indicated that essox3 and essox22 shown high homology to human sox genes, and the identities to human sox genes in dna sequence and amino acid sequence are 84 % 、 85 % and 97 % 、 81 %, respectively. it might be concluded that sox gene was highly conservative in phylogenesis
二、研究論文1 、參照人sry基因hmg - box保守區的序列,設計一對兼并引物, pcr擴增了中華絨螯蟹的sox基因,並對擴增產物進行了克隆和測序。結果在雌雄個體中篩選出兩個不同的sox基因essox3和essox22 ,其dna序列和編碼的氨基酸序列與人相應sox基因的相似性分別為84 % 、 85 %和97 % 、 81 % ,顯示該基因在進化上具高度的保守性。In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported
在此基礎上,根據國內外已發表的ibv基因序列,分別設計特異性引物,應用不同引物進行反轉錄合成cdna ,分片段對ibv的主要結構基因進行pcr擴增,並分別將各個目的片段克隆到puc19載體上,在大腸桿菌dh5中實現目的基因的分子克隆,經藍白斑篩選、限制性內切酶分析、 pcr鑒定,篩選出重組陽性質粒,並對各個目的基因片段進行序列測定,從而獲得ibv主要結構基因全序列。7. at the first time, the reporter dye, fam was linked to the 5 " - end of the oligonucleotides of the probes, and the tamra was located at the 3 " - end as quencher dye. we use camv35s and fmv promoter, nos terminater, mark gene nptii, and aim gene pat, epsps and cryla ( b ) genes as target sequences, design pairs of sp
7 、首次以fam熒光素標記探針5 』端作為發光基團,以tarma標記探針3 』端為淬滅基團,以camv35s 、 fmv啟動子、 nos終止子、標記基因nptll 、抗除草劑基因epsps 、 pat 、抗蟲基因cry1a )為檢狽目標,設計、篩選出特異性引物和探針,優化實驗參數,建立了轉基因植物通用性熒光pcr定性檢測方法體系。This procedure was simple, time - saving, highly sensitive and reproducible. based on the improved procedure, nearly 16, 000 cdna fragments were analyzed between the immature siliques of the wide type and ast mutant, and 28 differential cdna fragments were screened
採用這個改良的的方法,分析了擬南芥野生型和ast突變型植株未成熟角果中16 , 000個cdna擴增產物條帶,從中篩選出28個差異條帶。Drug sleuths can, for example, take advantage of standard genetic engineering ( introduced in the 1970s ) to produce pure copies of a selected protein for use in drug development
譬如說,藥物偵探便利用1970年代發明的遺傳工程標準技術,復制許多特別選出的病毒蛋白,做為藥物研發之用。All the pictures are selected by the association, chosen from thousands of images from various newspapers, magazines and news agencies. in addition, focus at the frontline 2006 exhibition, in which photographs are winners of the hkppa annual photo competition, will also be shown. three winners from each of the seven categories spot news, general news, people, sports, nature environment, feature and photo essays were selected
與此同時,已踏入第十四屆的前線焦點新聞攝影比賽亦會同場展出,是次比賽共收到逾千張參賽作品,是次比賽評判由各報章雜志的圖片部主管或編輯組成,于比賽的七個組別中:突發新聞、一般新聞、人物、體育、環境與自然、特寫及圖片故事選出各組冠、亞、季軍。Eighty - six press photographers participated in the focus at the frontline 2005 news photos contest, organized by the hong kong press photographers association and hong kong arts centre, submitting a total of 1200 photographs. three winners from each of the seven categories spot news, general news, people, sports, nature environment, feature and photo essays were selected
是次比賽評判由過去十一年比賽中獲獎最多的七位攝影記者組成,于比賽的七個組別中(突發新聞、一般新聞、人物、體育、環境與自然、特寫、圖片故事)選出各組冠、亞、季軍。And the adsorbability of this novel adso rbent for ldl was determined. in order to prepare the solid supports in accordance with with the request of adsorbent for ldl, paam beads were synthesized by inverse suspension polymerization. the composition of the polymerization system is shown as follows : the concentration of monomer and dispersant are 12 % and 0. 65 % respectively, the volume ratio of oil / water isl : 3, the mole ratio of aam and mba is 18 : 1
本文用反相懸浮聚合法合成符合ldl吸附劑載體要求的聚丙烯酰胺微珠,經實驗篩選出反應體系所需的最佳單體濃度( 12 ) 、最佳分散劑用量( 0 . 65 ) 、最佳油水比值( 1 : 3 ) 、最佳單體( aam )與交聯劑( mba )的物質的量之比( 18 : 1 ) 。Molecular design and luminescence studies of transition metal complexes with alkynyl - and chalcogen - containing ligands, a research project undertaken by professor vivian wing - wah yam, was chosen out of over 135 entries competed in the category of natural science this year
任詠華教授的得獎項目過渡金屬炔基及硫屬簇配合物的分子設計及其發光性能的研究是從135個參賽項目中被挑選出來的。Molecular design and luminescence studies of transition metal complexes with alkynyl - and chalcogen - containing ligands, a research project undertaken by professor vivian wing - wah yam, was chosen out of over 135 entries completed in the category of natural science this year
過渡金屬炔基及硫屬簇配合物的分子設計及其發光性能的研究是任詠華教授的得獎項目,是從135個參賽項目中被挑選出來的。Application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats to screen the exact, dependable, particular genetic monitoring marker method of laboratory animal, the author had studied the application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats, and compared the two methods with the biochemical marker enzyme method. the study had established the foundation of the molecular genetic monitoring marker method of laboratory animal
本文通過對dna指紋技術和pcr擴增微衛星dna技術在近交系大、小鼠遺傳檢測中的應用研究,並與生化位點標記分析法進行比較,旨在篩選出具有精確、可靠、特異性好的實驗動物遺傳檢測方法,為建立分子生物學實驗動物遺傳質量監測技術和標準奠定基礎。The " ho " in " hodori " comes from the korean word for tiger, and " dori " is a common masculine diminutive
吉祥物的名字採取了社會徵集的辦法, hodori是從2295個建議來稿中挑選出來的。分享友人