遺傳轉活化 的英文怎麼說

中文拼音 [yízhuànzhuǎnhuóhuà]
遺傳轉活化 英文
genetic transactivation
  • 遺 : 遺動詞[書面語] （贈與） offer as a gift; make a present of sth : 遺之千金 present sb with a gener...
  • 傳 : 傳名詞1 （解釋經文的著作） commentaries on classics 2 （傳記） biography 3 （敘述歷史故事的作品）...
  • 轉 : 轉構詞成分。
  • 活 : Ⅰ動詞1 （生存; 有生命） live 2 [書面語]（救活） save （the life of a person）:活人無算 （of a goo...
  • 遺傳 : [生物學] heredity; hereditary; inheritance; inherit
  • 活化 : [化學] activation radioactivation; activate活化處理 activating treatment; 活化分析 [核物理] activ...
  1. In this study, the screening and identification of the different agrobacterium rhizogenes strains were carried out using physiological, chemical, molecular and bioassay methods, and the vigor of different strains was also compared based on their characters ; the transformation of torenia mediated by agrobacterium rhizogenes, the hairy root formation and its regeneration were importantly studied

    本實驗研究了（ 1 ）發根農桿菌的生理生化、分子生物學鑒定及生物檢測和活力比較； （ 2 ）發根農桿菌介導的藍豬耳遺傳轉化和藍豬耳毛狀根的植株再生，為藍豬耳的遺傳轉化和其它分子生物學研究奠定基礎。
  2. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  3. The article intend to analysis the precess, character and motivion of development in traditional street space in which contain abundant cultural heritage. through investigation the writer should analysis the characte r, form and the relationship with each other, and furthermore find the pattern of the space in the fulture. what the most important is to summarize the spitits in traditional street space, such as activition, fuction, cultural elements, social customs and quatity of space. in order to put these elements to the exploit of new street space and find how these elements should be used, the writer regards some streets as the examples such as wenming street in kunming, xinhua street in lijiang jianshe rode in luxi. through research the writer find problems which exist in not only traditional but also in new street and express individual viewpoint about how to solve the problems. there are several ways to keep the new street exploition success. in the first place, we shoule know about all kinds of influences and synthesized elements. secondly mastering the mechanism of the development by oneself is indispensable. in the last place, it is necessory not to imitate some certain model which is plausible in other place. in the l ast of the article, the writer uses a new project to further illustrate the way to put the theory what have mentioned above into the practice

    並從自然，社會，經濟及社會文化方面分析產生的根源，發展動力，其次通過實際調查，對現有傳統商住的物質形態要素進行分析，從商空間及住空間的基本特徵，型制入手，探索傳統商住街區的空間形態，結構組成，商與住空間關系，空間性質及空間如何相互轉化。以此來尋找傳統商住街區物質形態特徵，同時結合具體實例強調在傳統商住街區改造、新區的規劃設計中應當重視非物質要素內涵的提取、如活動者及社會結構、功能意義、空間品質、文化氣息，保持歷史傳統的延續性的必要性與可行性。在文章的後半部分，有針對性的對遺留下來的傳統商住街區-昆明文明街、麗江新華街、瀘西建設路的物質形態特徵和非物質形態特徵的分析，找出現存的問題，並針對問題發表個人的觀點，指出引導現代商住街區的開發與改造中，保持形態合理發展的正確途徑是了解影響形態發展與演變的各種因素及其綜合作用，把握好形態演交的內在機制，而不應刻意追求某種特定的模式。
  4. The library needs be understudied what is the business of humanism ; it is the important mechanism of protecting social heredity ; it is the medium of social knowledge with personal knowledge convert mutually it is the measure of " quantity of life " what library ' s construction and usage, and rise the important function of fill up " digital divide " ; is the important measures of the human society advances, and is the history of society development too

    摘要圖書館需要理解，它是人文主義的事業；圖書館是保護社會遺傳的重要機制，它是社會知識和個人轉化的重要中介；圖書館建設與利用是人類生活質量高低的一個表徵，它在彌合「信息鴻溝」中起著重要作用；圖書館是衡量人類社會進步的重要標度，圖書館的發展史也是社會發展史。
  5. The library needs be understudied what is the business of humanism ; it is the important mechanism of protecting social heredity ; it is the medium of social knowledge with personal knowledge convert mutually ; it is the measure of " quantity of life " what library ' s construction and usage, and rise the important function of fill up " digital divide " ; is the important measures of the human society advances, and is the history of society development too

    圖書館需要理解，它是人文主義的事業;圖書館是保護社會遺傳的重要機制，它是社會知識和個人轉化的重要中介;圖書館建設與利用是人類生活質量高低的一個表徵，它在彌合「信息鴻溝」中起著重要作用;圖書館是衡量人類社會進步的重要標度，圖書館的發展史也是社會發展史。
  6. Mint total dna was transferred into a. thaliana mediated by ion beam with the influence of 0. 5 10 1. 5 1017 and 2. 5 1017 ions / cm2, respectively, which were on the bridge of saddle. among three transferred populations, their budding, seedling, growth and phenotypic variation took on obvious difference. combining these facts, the influence of 1. 5 1017ions / cm2 was decided as transformation influence of the following work

    我們從另外一個角度出發，在離子注入劑量-擬南芥菜存活曲線的基礎上，選擇不同的離子劑量介導薄荷全dna轉化擬南芥菜，根據各個轉化群體在遺傳和生理上的不同變化，選擇1 . 5 10 ~ （ 17 ） ions cm ~ 2作為我們以後轉化工作的轉化劑量。
  7. With the precondition that invitro tissue could survive after implantation, the study on transferring gus gene into arabidopsis callus was done. and we got the transformants. transformation efficiency was up to 2 %

    在活體組織注入后可以存活的前提下，通過對離子束介導質粒dna導入擬南芥愈傷組織的遺傳體系的研究，得到了gus基因轉入擬南芥愈傷組織的轉化植株，轉化效率達2 。
  8. Though the technique of nuclejc transformation in plants has been developed and used widely, some problems in genetic information have not been resolved. for example, because the nucleic genome is so big and complicated that the integration sites and copies of foreign gene can not be controlled accurately, the expression of transferred genes is inefficient as a result of gene silencing or position effect. in nucleic transformation, furthermore, the transfer of multigene is difficult, and only after the prokaryotic genes undergo modification are they expressed in high plants

    植物的細胞核轉化技術已發展成熟並得到廣泛應用，但核基因組的遺傳轉化仍存在一系列至今尚未解決的問題：例如由於核基因組大，背景復雜，外源基因的整合位點和整合的拷貝數難以人為控制，造成鄭州大學2003年博士學位論文杜氏鹽藻（ dunaliellasalina ）葉綠體轉化研究外源基因表達效率低，容易出現基因失活、基因沉默、位置效應等現象;同時轉入多個基因時操作步驟過于復雜，所表達的原核基因必須經過修飾改造，環境安全難以保證等。
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