酶作用基 的英文怎麼說

In trpsin tolerance assay. this virus could resist to 1 % trpsis at 37 in an hour. in acid tolerance assay, this virus was resistant to ph3. 0 and ph5. 0 at 37 in 2 hours, and the average infection litre of the virus decreased little. in heat assay, at 50, the virus was processed from 5 minutes to 150 minutes and at each condition the viral virulence reduced to some certain degree. among these conditions, when at 50 in 30 minutes. the average infection litre of this virus decreased over 2 tilre. and when al 50 in an hour, cpe of ihis virus disappeared. when time was set for an hour. but with processed in different temperature as 50 60 70, 80, the virus losl the multiplication capacity complelely. in biological assay, we selected different cell lines to cultivate this virus by laking advantage of possesional cells at that time in our laboratory. then we found that fcwf cell line was the most sensitive to dxmv and mdck was the second. with f81 cell line, after passaged for 12 times continuously with low concentration of fcs. the virus could produce cpe. however, with vero cell line. the virus could not procuce any cpe after many passages. the hemagglutination and lumadsorption reaction test proved that this virus had no any reaction to erythrocyte of pig, fowl and cavy. by neutrolizaion assay, dxmv could be identified as a kind of ccv

理化學研究表明，該病毒為rna病毒，對氯仿、乙醚敏感；胰酶試驗中，經37 、 1小時處理的病毒，仍然能夠在貓源細胞fcwf細胞上生長，並且毒力基本保持不變；耐酸性試驗中，病毒分別在ph5 . 0和ph3 . 0經37作用2小時，毒力僅下降一個滴度；耐熱性試驗中，該病毒在恆定溫度50 ，設定不同時間，從5分鐘到150分鐘，毒力均有不同程度下降，其中， 50作用30分鐘，病毒平均滴度下降2個單位； 50 ， 60分鐘， cpe消失；恆定時間1小時，設定不同溫度（ 50 - 60 - 70 - 80 ） ，病毒在細胞上完全喪失增殖能力， cpe消失。生物學試驗，利用實驗室現有條件，選擇不同的細胞系對該病毒進行培養，發現該病毒對貓源細胞fcwf最敏感； mdck細胞次之； f81細胞經多次傳代，亦可出現cpe ；而vero細胞則不敏感。血凝試驗表明，該病毒對豬、雞、人及豚鼠的紅細胞均無血凝性。

Researches of schistosomiasis vaccines have gone more than 60 years, approximately including from the stages of dead vaccine and live vaccine ( irradiated attenuated cercariae vaccine ) to gene engineered vaccine, etc. many different forms of vaccines have been tested in animal models, including gluthathione s - transferase, paramyosin, irv - 5, triose phosphate isomerase, sm23, fatty acid binding protein ; which were considered promising by who / tdr. but none of them steadily accomplished the pre - set target level of 40 % protection. in order to enhance the protective capacity further, it is essential to develop novel vaccine antigens and / or vaccine adjuvants

血吸蟲病疫苗研究已有60多年的歷史，大致經歷了死疫苗、活疫苗（照射致弱尾蚴疫苗）和基因工程疫苗等研究階段，產生了一些who / tdr推薦認為很有希望的疫苗候選分子，如谷胱甘肽- s -轉移酶（ gst ） 、副肌球蛋白（ sm97 ） 、照射致弱疫苗抗原5 （ irv - 5 ） 、磷酸丙糖異構酶（ tpi ） 、曼氏血吸蟲膜內在蛋白（ sm23 ）和脂肪酸結合蛋白（ fabp ， sm14 ）等，但其對宿主的保護作用均不甚理想，未能穩定地達到40或以上的保護力水平，因此有必要繼續尋找新的疫苗抗原分子和/或疫苗佐劑，進一步提高其保護力。

The complex formed by cnbr - activated alginate and antibody is aggregated to the surface of the paraffin - graphite - chitosan electrode by electrostatic adsorption ( coacervation ). the concentration of sjag can be detected by determining the redox current of o - aminophenol, which oxidized by h2o2 in the presence of hrp. moreover, the immunosensor shows some improved performances including high sensitivity, selectivity and less non - specific adsorption

褐藻酸鈉?抗體復合物通過靜電吸附作用被凝集到含石墨?石蠟?殼聚糖組分的電極表面，然後與抗原和酶標抗原進行競爭反應，以鄰氨基酚為電子媒介，通過測定酶催化下雙氧水對其氧化的電流大小來間接測定抗原的濃度。

Gene engineering technology is more superior than the cross breeding and directive breeding technology with its short cycle, low cost and high benefit. though traditional breeding technology has been used for a long time. now the direct reports for the changes of the flower color by the chi ( chalcone isomerase ) gene are a few what we known.

關于花色結構基因查爾酮異構酶chi （ chalconeisomerase ）基因對花色改變的直接報道很少，因此，本論文選用了chi基因為目的基因，以純深紅色和純白色矮牽牛（ petuniahybidavilm . ）為材料，研究了chi基因的共抑制和反義抑制以及表達產物增加對花色改良的作用，並在花色改變植株中首次觀察到花器官變異。

The changes of these endopeptidase are different during the former vasing of " samatha " and " belami ". the main eps in petals of " samatha " are the acid thiol - proteinase, metallo - proteinase and the alkali serine proteinase and the main eps in petals of " belami " are the acid thiol - proteinase and serine proteinase. during the later vasing the acid metallo - proteinase and the alkali serine proteinase become main eps in petals of " samatha " and " belami "

這3種內肽酶在兩種切花瓶插初期（盛開以前）的表現不同： 『薩蔓莎』花瓣中以酸性金屬蛋白酶、巰基蛋白酶和堿性絲氨酸蛋白酶起主要作用， 『貝拉米』花瓣以酸性巰基蛋白酶、絲氨酸蛋白酶起主要作用，後期『薩蔓莎』和『貝拉米』花瓣均以酸性金屬蛋白酶、堿性絲氨酸蛋白酶起主要作用。

Fad ( flavin adenine dinucleotide ) a derivative of riboflavin that is a coenzyme in electron - transfer reactions

黃素腺嘌呤二核苷酸（ fad ） ：核黃素的衍生物，在電子傳遞反應中是一種輔酶，它作為各種脫氫酶的輔助基團起作用。

Abstract : numerous mushrooms are toxic to insects. to identify the chemicals involved in insecticidal activity, the toxicity of 14 species has b een studied for water solubility, thermolability, and dialysis. the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects. among proteins, lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease

文摘:許多蘑菇都對昆蟲表現出毒性.為了證實與殺蟲毒性有關的化合物，對14種蘑菇的毒性在水溶性、熱敏性和可透析性等方面進行了研究.研究數據表明，蛋白質對大多數蘑菇子實體的殺蟲活性起著重要作用，也許是一種可以用於植物抵抗害蟲的基因源.在數種蛋白質中，凝集素和溶血素因不受蛋白酶的影響而成為良好的殺蟲劑候選材料

Numerous mushrooms are toxic to insects. to identify the chemicals involved in insecticidal activity, the toxicity of 14 species has b een studied for water solubility, thermolability, and dialysis. the data strongly s uggest that proteins are responsible for most of the insecticidal avtivity in mu shroom fruitbodies and may be a source of genes available for plant protection a gainst insects. among proteins, lectins and haemolysins are good insecticide candi dates because the toxicities are not affected by protease

許多蘑菇都對昆蟲表現出毒性.為了證實與殺蟲毒性有關的化合物，對14種蘑菇的毒性在水溶性、熱敏性和可透析性等方面進行了研究.研究數據表明，蛋白質對大多數蘑菇子實體的殺蟲活性起著重要作用，也許是一種可以用於植物抵抗害蟲的基因源.在數種蛋白質中，凝集素和溶血素因不受蛋白酶的影響而成為良好的殺蟲劑候選材料

Other : the dyewood is known as the suppression histidine decarboxylase, the catecha phenol - o - methyl shift enzyme action

其它:染料木素有抑制組氨酸脫羧酶，兒茶酚- o -甲基轉移酶作用

Now transgenic cell lines that express the protein encoded by ugt gene were broadly used instead of liver microsomes. the enzyme expressed in cell lines helps to realize the function of a specific gene and will provide direct information related to the isozyme interested

『為解決上述問題，國際上人們己經廣泛採用建立各種ugt轉基因細胞系的方式試驗，在明確基因構成的倩況下直接獲得由特定基因所表達的ugt同工酶， fi什對性地研究該酶對藥物的代謝作用。

Found the phenomenon observed in the nematode caenorhabditis elegans for the first time in 1998, consequently similar processes have been described for drosophila melanogaster, trypanosome, mammals including humans. the mechanism is that sirnas is the mediator, which can induce the risc to the target mrna and degrade it. recently there was great progress in the specific gene therapy and anti - virus, and rnai has been a focus of rna molecular therapy

自1998年fire等在研究線蟲時首次發現以來，相繼在果蠅、渦蟲、錐蟲、小鼠及哺乳動物細胞中發現rnai現象。一般認為： rnai第四軍醫大學碩士學位論文效應作用機制是sirnaskmallinterferinguaduplex ）作為中介分于，引導risc （ rnaiinducingsuppresscomplex ）至靶基因m洲a處，隨后核酸內切酶將之降解。

It will provide us to further study the function of xanthophyll cycle in photoprotection. the major results are as following : two cdna sequences encoding violaxanthin de - epoxidase were cloned from japonica rice ( jrvde ) and indica rice ( irvde ) with the full - length of 1887bp and 1647bp, respectively. the homology of the open reading frame is 98 % identity between two rvde genes, and more than 60 % identities with those of other species

本論文從水稻和菠菜中克隆了編碼vde酶的基因，並通過轉基因植物進一步研究了葉黃素循環在熱耗散方面的作用，主要獲得了以下結果：首次從兩個水稻亞種（秈稻和粳稻）中克隆了rvde基因（分別命名為irvde和jrvde ）的全長cdna序列，分別長1647bp和1887bp ，兩者開放閱讀框的同源性為98 ，與其它已知vde基因的同源性在60以上。

The enzyme digest analysis shows that the arm repeats of c - terminal are conceivably conservative domain. in arc1 protein, there are some active sites including n - glycosylation sites, camp - and cgmp - dependent protein kinase phosphorylation sites, protein kinase c phosphorylation sites, casein kinase ii phosphorylation sites, tyrosine kinase phosphorylation sites, n - myristoylation sites, amidation sites and leucine zipper pattern. it probably take part in the signaling process of self - incompatibility

同時在arc1蛋白質中還發現了拉鏈結構和多個磷酸化位點，包括camp和cgmp依賴的蛋白激酶磷酸化位點、蛋白激酶c磷酸化位點、酪蛋白激酶磷酸化位點、酪氨酸激酶磷酸化位點、糖基化位點等，拉鏈結構為arc1蛋白之間及與其它蛋白的相互作用提供了可能，而磷酸化位點是arc1參與信號傳導過程所必需的。

Soil microbial biomass and viable population size ( plant counts ) were negatively affected by the elevated metal levels, but the size of soil basal respiration rate and microbial metabolic quotients were positively influenced by the increasing heavy metal pollution levels. microbial community structure also changed with increasing contamination, as indicated by biolog data and principal component analysis of biolog community metabolic profiles. soil microbial metabolic profiles ( awcd ) values, community richness and diversity index in mine - soils decreased remarkably as compared

相關分析結果表明，土壤重金屬含量和土壤基礎呼吸、微生物量cfn 、代謝剖面（ awcd ） 、微生物商（ cmic / corg ） 、代謝商（ qc02 ）與人工栽培的香根草植物地上部分呈顯著或極顯著正相關（卜0 . 6653飛0 . 8945 」 ） ;微生物量c 、微生物量n 、生化作用強度、酶活性、群落shannon指數（ h ）和微生物群落豐富度（ s ）與人工栽培的香根草植物地上部分生物量呈顯著或極顯著地負相關（ r =一。

Such examples are given as the blood filtering principal of hemoglobin, catalysis of enzymes, immune recoglization, prion, glycoprotein and the relationship of structure and function of membrane protein, et al, as well as the applications to medicine

在簡要介紹結構生物學的研究方法的基礎上，主要從分子水平闡述蛋白質和核酸的結構原理、相互作用、結構與功能的關系，通過具體實例闡述血紅蛋白的輸氧機制、酶的催化機制、免疫分子識別、朊病毒、糖蛋白、生物膜的結構功能關系等，以及結構生物學在醫學上的應用。

The a - transglucosidase was selectively modified by pcmb, me, edc, clac, acetyl acetone and nbs, and changes in the activities of the enzyme have been detected. the reaction of a - transglucosidase with pcmb, me, edc and clac resulted in a strong inhibition of the enzyme activities which decreased with the increase of modifier concentration. the acetyl acetone and nbs were found without inhibition effect

酚丙酮、小涅代唬拍酷亞胺剛b引等化學修飾劑對a葡萄糖轉苦酶的幾種氨基酸殘基進行選擇修飾，並測定其酶活力變化，結果表明： pcmb 、 me 、 edc 、 ciac能顯著抑制酶的活性，活力的下降與修飾劑的濃度有關，乙酚丙酮、 nbs白巾飾對酶的奪製作用不明顯。

The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。

To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively

鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。

This review briefly summarizes the research progress on phytases from bacillus subtilis in effect mechanism, property, isolation, purification and taxonomy of the enzyme and gene engineering to speed up the deep research and exploitation of the enzyme

主要綜述了枯草芽孢桿菌植酸酶作用機理、酶學性質、分離純化、分類地位和基因工程方面的研究進展，以便為該酶的深入研究和應用提供借鑒作用。

A. niger m - l which was screened in our laboratory produced a strongly a - transglucosidase. in this paper, studies on the fermentation conditions, purification and characterization of a - transglucosidase and its necessary groups was carried out in this dissertation. the main reports were as following : the fermentation conditions in shaking flasks were investigated by the method of single - factor analysis, the suitable main medium was achieved : which contained 4 % a, 2 % b and 1 % g ; the a. niger m - l was inoculated into 100ml medium in flask, shaking in 33 c at 140r / min for four days, with initial ph6. 5 and 6 % inocula volume ; adding 0. 1 mmol / l methyl a - d - glucopyranoside had inductive effect on enzyme formation, the a - transglucosidase activity amounted to 296. 05u / ml

本研究以黑麴黴m - 1為出發菌株，對其-葡萄糖轉苷酶的產酶影響因素、純化、酶學性質以及必需基團進行系統的研究，結果如下：通過對影響黑麴黴m - 1產-葡萄糖轉苷酶的單因素分析，得液態發酵生產-葡萄糖轉苷酶的最適產酶條件為： 4 a ， 2 b和1 g ；在33 ，起始ph值為6 . 5 ，轉速為140r min ，接種量為6 ，裝液量100ml條件下，發酵4 . 0d ，酶活力達296 . 05u ml ，添加0 . 1mmol l的酶作用底物甲基- - d -葡萄糖苷對產酶的誘導作用最大。