酶分子生物學 的英文怎麼說
中文拼音 [fēnzishēngwùxué]
酶分子生物學
英文
enjymology- 酶 : 名詞[生物化學] (生物體的細胞產生的有機膠狀物質) enzyme; ferment
- 分 : 分Ⅰ名詞1. (成分) component 2. (職責和權利的限度) what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] (料想) judge
- 子 : 子Ⅰ名詞1 (兒子) son 2 (人的通稱) person 3 (古代特指有學問的男人) ancient title of respect f...
- 生 : Ⅰ動詞1 (生育; 生殖) give birth to; bear 2 (出生) be born 3 (生長) grow 4 (生存; 活) live;...
- 物 : 名詞1 (東西) thing; matter; object 2 (指自己以外的人或與己相對的環境) other people; the outsi...
- 學 : Ⅰ動詞1 (學習) study; learn 2 (模仿) imitate; mimic Ⅱ名詞1 (學問) learning; knowledge 2 (學...
- 生物學 : biology
- 生物 : living things; living beings; organisms; bios (pl bioi bioses); biont; thing; life生物材料 biol...
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It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity
本研究中,木耳屬2個種的2個菌株在its區域表現出較高的保守性, 4種限制型內切酶的酶切圖譜沒有顯示出多態性;增加內切酶種類及供試菌株數量,有可能獲得具有多態性的限制性內切酶酶切圖譜; 9本實驗中, its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異,擴增片段長度均為650bp左右; 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析,紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法,兩者的關系是相輔相成,互為驗證Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板,用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增,得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上,篩選反向克隆,然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游,構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上,通過花粉管通道法轉化河套蜜瓜,共獲76顆瓜,並進行了硬度和含糖量的分析。2. 5 ul of 10 x reaction buffer, 1. 5 ul 25mm mgcl2, 0. 3 ul lomm dntp, 0. 5 ul taqdna polymerase ( 5 u / ul ), and lul ( = 20ng ) of primer were used in per reaction. each reaction was overlaid with one drop of paraffin oil. the initial denaturation step was used at 94 for 1 min 45 sec ; then denatured at 94 for 30 sec, annealed at 37 1 min, extended at iv b 72 for 2 min and repeated the cycle 45 times, at last, extended at 72 ' c for lomin
等( 1995 )利用rapd標記區分美國東部一雜交地帶的蟋蟀的兩個姐妹種, allonemobiusfasciatus和a . socius ,並於1998年使用rapd和異型酶標ic做出了這兩種蟋蟀的基因連鎖圖;國內田英芳、鄭哲民( 20of )首次將rapd技術運用於蟋蟀總科的分子系統學研究中,採用2種引物對7種蟋蟀進行了基困組dna多態性研究,並應用upg問a法構建樹狀圖,椎測系統發生關系Based on the previous studies, the research in this paper was carried out, mainly including two parts as follows : ( 1 ) anammox bacteria and aerobic ammonia oxidizers were detected in situ in 6 sediment samples taken from jiangsu province. molecular techniques, such as fish, pcr, dna cloning and sequencing etc. were used for this purpose. ( 2 ) the continuous cultivation of anammox bacteria from sediment samples were studied, which provides experimental basis for the bioaugamentation of eutrophicated sediment applying anammox process
本論文在前人研究的基礎上,開展了以下兩個方面的工作: ( 1 )採用分子生物學技術熒光原位雜交( fish ) 、多聚酶鏈式反應( pcr ) 、 dna克隆和測序等對采自江蘇省蘇州市、東太湖、新沂河等6個底質樣品進行了厭氧氨氧化菌和傳統氨氧化菌的原位檢測; ( 2 )探討了以底質作為接種體進行厭氧氨氧化菌富集培養的可行性,為天然底質環境中厭氧氨氧化過程的強化,富營養化底質微生物修復的可行性提供一定的依據。The type ii pha biosynthesis genes cloned from b. caryophylli ys13 using the described pcr method demonstrated that pha biosynthesis in burkholderia strain has an additional pathway to the normally type i pathway
對bcaryophylliys的分子生物學研究發現,它擁有類似於假單胞菌的型pha合酶體系。Along with the development of the cytobiology and the molecular biology, and thoroughly research of the biophysics, the biochemistry, the genetics and immunology, it has cultivated the modem biological technology, such al genetic engineering, cellular engineering, enzyme engineering, fermentation engineering and so on, to change biology characteristic to carry on the material transformation, has formed the front biological examination technology : the dna probe, the pcr technology, the molecular mark, the bioluminescence technology, genechip technology and so on the widespread application of these advanced biotechnologies in dairy industry baa impelled the dairying technical transformation, and has been having vital significance to dairy production, research and dairy product security
摘要隨著細胞生物學和分子生物學的發展及對生物物理、生物化學、遺傳學和免疫學研究的深入,培育了基因工程、細胞工程、酶工程、發酵工程等改變生物特性進行物質轉化的現代生物技術,形成了dna探針、 pcr技術、分子標記、生物熒光技術、基因晶元技術等前沿性的生物檢測技術,其在乳品工業中的廣泛應用,推動了乳業的技術變革,對乳品生產、研究和乳品安全意義重大。The a - complementation reaction of & - galactosidase ea and ed was also used in dna cloning, protein protein interactions monitoring and expressing immunoassays studying
-半乳糖苷酶的ea 、 ed這種-互補性還被用於分子生物學、蛋白質相互作用的監控、表達免疫分析等方面的研究。A review about current progress in understanding biochemical processes of starch synthesis in developing cereal endosperms, with an emphasis on the molecular biology aspect of key enzymes involved in the processes
綜述了禾穀類作物胚乳澱粉合成生化過程的近期研究進展,對澱粉合成關鍵酶的生理和分子生物學研究現狀做了重點介紹。The study, which is published in the current issue of nature structural and molecular biology, inestigates the structure and function of the protein and sheds light on how polymerase mutations contribute to transmission of aian flu to humans
這項研究出版在自然結構和分子生物學現期雜志上,它探究了這種蛋白質的結構和功能並且使多聚酶是怎麼突變以促使禽流感傳播到人的清楚地顯示出來In the laboratory experiment part, human peripheral blood, cultured cells and icr mice were study objects. the changes of mitotic chromosome numbers were measured by human metaphase chromosome counts and statistic analyzed used x2 - test. the changes of meiotic chromosome numbers were measured by mice one - cell zygote chromosome counts and statistic analyzed usedx2 - test. the effects of low dose ionizing radiation on the expression of topoisomerase ii were measured by immunocytochemistry, western blot and rt - pcr
流行病學結果顯示長期小劑量輻射接觸與染色體不分離呈正相關,為進一步在細胞遺傳學和分子生物學方面研究小劑量電離輻射與染色體不分離關系及其機制,本課題第二部分以外周血、培養細胞、 icr小鼠為研究對象,用外周血染色體計數和單細胞受精卵染色體計數的方法研究小劑量輻射和拓撲異構酶復旦大學2000級博士生學位論文11a抑制劑及其二者的協同效應對有絲分裂和減數分裂染色體不分離的影響,用免疫細胞化學染色、 westernblot 、 rt pcr等方法研究了電離輻射引起拓撲異構酶a表達變化。In order to break down the rate - limited steps in the artemisinin biosynthesis to improve the artemisinin production and realize the industrial production of artemisinin, related key genes in artemisinin biosynthesis must be cloned and the regulatory patterns of key genes should be studied. for this purpose molecular cloning of related key genes in artemisinin biosynthesis was performed in this thesis work
利用現代分子生物學和基因工程技術手段,克隆青蒿素生成途徑的關鍵酶基因,研究關鍵酶基因對青蒿素生物合成的調控規律,是打破青蒿素生物合成的限速步驟,大幅度提高青蒿素含量,最終達到利用植物生物技術工業化生產青蒿素的目的必須解決的關鍵問題。For example, in 20 centuries 70 time, as the progress of molecular biology, be united in wedlock with project technology photograph, open up biology technology new field : gene recombines technology of cross of technology, pcr technology, dna and technology of protein alignment analysis, element to wait, the birth that promoted gene project, protein project, cell project, enzymatic project to wait and development
例如,在20世紀70年代,隨著分子生物學的進步,與工程技術相結合,開辟了生物技術新領域:基因重組技術、 pcr技術、 dna和蛋白質序列分析技術、分子雜交技術等,促進了基因工程、蛋白質工程、細胞工程、酶工程等的誕生和發展。The aim of this thesis was to investigate the development, distribution, properties and metabolic factors of digestive enzyme in alimentary tract by means of methods of enzymatic biochemistry and molecular biology, which provided theoretic evidence for formulation of feed and nutritional requirement in tilapia
羅非魚具有優良的生長特性和飼料利用能力,本文採用酶學和分子生物學的研究技術,對羅非魚消化酶的特性、分佈和活性變化規律以及與外源因子的相互關系進行了系統研究。In order to understand the epidemiological behaviors of this pathogen, we studied strains e. coli 0157 isolated from patients and dung beetles in 1999 and 2000 in xuzhou city, jiangsu province, by methods of pcr, antimicrobial susceptibility, biochemical reaction and pfge ( pulse - field gel electrophoresis ) of chromosomal dna digested by restriction enzyme xbal
為了探索我國大腸桿菌o157 : h7感染的流行病學特點,我們使用了聚合酶鏈反應、生化反應、抗生素敏感實驗、脈沖電場凝膠電泳等分子生物學方法,對我國1999 - 2000年徐州地區分離到的部分大腸桿菌o157 : h7進行了分析。Objective : to detect the telomerase activity in human esophageal carcinoma and tumor ? adjacent tissues and investigate the possible molecular mechanism of esophageal carcinogenesis
目的:檢測食管癌及周圍非癌食管粘膜組織中端粒酶活性,了解食管癌發生可能的分子生物學基礎。The progress for pectinase molecular biology promoted the development of its purification and assay methods
果膠酶分子生物學的迅速發展極大地促進了分離純化與分析方法的研究。1. expression of cry genes located in native plasmid in different flagella serotype strains to study cloning and expression of icps genes, an ecor i - f fragment of cryla ( a ) gene from pesi was inserted into pselect - 1 with t7 rna polymerase promoter in vitro. the plasmids of bacillus thuring fensisybt - 803 and ybt - 791 were analyzed by southern hybridization using an rna probe of ecori - f fragment and by pcr identification with cryl mixture primers
將cry基因的高保守區的cry a ( a ) ecog - f片段插入帶有t7rna聚合酶啟動子的質粒pselect - 1 ,獲得了能在體外轉錄的rna探針載體pbpl - 1 ,用該載體制備的rna探針具有特異強,背景清楚,省時省力等優點,已成功地用於蘇雲金芽胞桿菌的分子生物學研究和特異菌株的篩選。The human placental alkaline phosphatase ( hplap ) ( ec 3. 1. 3. 1 ) would be used more widely in molecular biology and elisa for its enzyme activity and heat resistant was higher than that of e. coli alkaline phosphatase ( eap ) and calf alkaline phospha - tase ( c1ap ). it is promising to construct the gene engineering pichia pastoris to product the hplap in a scale for the resource of hplap is limited
L )的活性和耐熱性都高於常用的大腸桿菌堿性磷酸酯酶( eap )和小牛腸堿性磷酸酯酶( ciap ) ,因而更適用於分子生物學和酶聯免疫領域,然而其米源受到限制,探討利用畢赤酵母尺屍astorjs構建工程菌進行規模生產鳳有) 』 『闊的前景。Microorganism chitinases : molecular biology and potential use as biopesticides
微生物幾丁質酶的分子生物學研究及其應用Gala apples would be stored in cold storage, air condition and ultro - lower storage for 55 days, 107 days and 161 days, respectively. alkaline phosphatase is an important tool enzyme in molecular biology and elisa
杜中軍征果多閑p c測定方法及堿性磷酸酯酶在畢赤酵母中表達體系的建立堿性磷酸酯酶是分子生物學領域和酶聯兔疫分析中常用的工具酶之一,人胎盤堿性磷酸酯酶( hplap ) ( ec3分享友人