酶和酶工程 的英文怎麼說

中文拼音 [hégōngchéng]
酶和酶工程 英文
enzymes and it』s engineering
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 和 : 和動詞（在粉狀物中加液體攪拌或揉弄使有黏性） mix （powder） with water, etc. : 和點兒灰泥 prepare some plaster
  • 工 : Ⅰ名詞1 （工人和工人階級） worker; workman; the working class 2 （工作; 生產勞動） work; labour 3 ...
  • 程 : 名詞1 （規章; 法式） rule; regulation 2 （進度; 程序） order; procedure 3 （路途; 一段路） journe...
  1. Researches of schistosomiasis vaccines have gone more than 60 years, approximately including from the stages of dead vaccine and live vaccine ( irradiated attenuated cercariae vaccine ) to gene engineered vaccine, etc. many different forms of vaccines have been tested in animal models, including gluthathione s - transferase, paramyosin, irv - 5, triose phosphate isomerase, sm23, fatty acid binding protein ; which were considered promising by who / tdr. but none of them steadily accomplished the pre - set target level of 40 % protection. in order to enhance the protective capacity further, it is essential to develop novel vaccine antigens and / or vaccine adjuvants

    血吸蟲病疫苗研究已有60多年的歷史，大致經歷了死疫苗、活疫苗（照射致弱尾蚴疫苗）和基因工程疫苗等研究階段，產生了一些who / tdr推薦認為很有希望的疫苗候選分子，如谷胱甘肽- s -轉移酶（ gst ） 、副肌球蛋白（ sm97 ） 、照射致弱疫苗抗原5 （ irv - 5 ） 、磷酸丙糖異構酶（ tpi ） 、曼氏血吸蟲膜內在蛋白（ sm23 ）和脂肪酸結合蛋白（ fabp ， sm14 ）等，但其對宿主的保護作用均不甚理想，未能穩定地達到40或以上的保護力水平，因此有必要繼續尋找新的疫苗抗原分子和/或疫苗佐劑，進一步提高其保護力。
  2. Sub - - clone of s, . / hbsag fusion gene : pbuescripts, . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme, and were linked under t4 dna ligase, ppiczaa s, / hbsag was constructed and transformed to e. coli

    Hbsag質粒與ppiczaa載體分別經xhol和xbaln切，再在t4dna連接酶作用下進行連接，獲得工程菌表達型ppiczaas ； hbsag質粒，轉化大腸桿菌t0p10細胞，經xhol和xbal與sacll和xbal酶切電泳，證實s ； 。
  3. The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn

    用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌，通過農桿菌介導法和基因槍轉化法轉化龍牙百合，經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較，發現農桿菌介導法的轉化率為16 . 7 ，基因槍法的轉化率為50 ，因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。
  4. The cowpea trypsin inhibitor ( cptt ) gene is testified as a broad spectrum insect - resistant gene at present and its application in insect - resistant botanic transgenic engineering only after s / gene. the cpti transgenic plant developed rapidly for it ' s broad spectrum insect - resistant character and the target insects are uneasy tolerance to it

    豇豆胰蛋白酶抑制劑（ cpti ）基因是目前在植物抗蟲基因工程中應用僅次於bt基因的廣譜性抗蟲基因。鑒於它抗害蟲的廣譜性和靶標昆蟲不易對其產生耐受性的優點，轉cpti基因植物得到了迅速的發展。
  5. Along with the development of the cytobiology and the molecular biology, and thoroughly research of the biophysics, the biochemistry, the genetics and immunology, it has cultivated the modem biological technology, such al genetic engineering, cellular engineering, enzyme engineering, fermentation engineering and so on, to change biology characteristic to carry on the material transformation, has formed the front biological examination technology : the dna probe, the pcr technology, the molecular mark, the bioluminescence technology, genechip technology and so on the widespread application of these advanced biotechnologies in dairy industry baa impelled the dairying technical transformation, and has been having vital significance to dairy production, research and dairy product security

    摘要隨著細胞生物學和分子生物學的發展及對生物物理、生物化學、遺傳學和免疫學研究的深入，培育了基因工程、細胞工程、酶工程、發酵工程等改變生物特性進行物質轉化的現代生物技術，形成了dna探針、 pcr技術、分子標記、生物熒光技術、基因晶元技術等前沿性的生物檢測技術，其在乳品工業中的廣泛應用，推動了乳業的技術變革，對乳品生產、研究和乳品安全意義重大。
  6. Protein engineering and site directed mutagenesis have been used to change the active site and alter the substrate specificity of various hydroxynitrile lyases

    研究人員已經利用蛋白質工程和定點突變技術來改變各種醇腈酶的活性位點和底物特異性等。
  7. This review emphasizes some important aspects for the control of lipase enantioselectivity : medium engineering, substrate engineering, modification of enzyme and directed evolution

    文章討論了調控脂肪酶對映體選擇性的幾種方法：反應介質工程、底物分子工程、酶分子修飾和定向進化。
  8. Its enzymatic activity showed less similarity with gloshedobin. therefore, these three residues " replacement may influence the stability of the advanced structure of the enzyme

    這兩類蛇毒類凝血酶基因的成功測序為其基因工程及其結構和功能的研究創造了條件。
  9. The paper reviews home and overseas research works on exhumation and use of nitrogen - fixing resource, genetics engineering of nitrogen fixation, and biochemistry of nitrogenase and chemical modeling of biological nitrogen fixation, and describe the prospect of biological nitrogen - fixing research

    本文從固氮資源的發掘和利用、固氮的遺傳工程以及固氮酶生物化學和化學模擬生物固氮三方面對國內外的研究進展進行了全面的闡述，並對生物固氮研究的前景進行了展望。
  10. This contributes to make clearly position and function of - glc in forming course of tea aroma, substantiate the theory that the aroma of tea forms and also establish the foundation that utilize genetic engineering techniques to improve the cultivar of tea plant, quality of tea and insect - resistance and disease - resistance afterwards. in the paper, the complete cdna squence of - glc of tea was cloned in the tea leaf at the first time

    因此，嘗試從茶葉中獲取-葡萄糖苷酶基因的cdna序列，將有助於進一步搞清-葡萄糖苷酶在茶葉花果香氣形成中的地位和作用，充實茶葉香氣形成的理論，也為以後利用基因工程手段改良茶樹品種，提高茶葉品質、抗病蟲能力以及該酶的轉化、調控的研究奠定基礎。
  11. When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum

    莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎，同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達，所構建的重組質粒ptga ，其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 ， 5 、 4 、 2 . 3倍，且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中，搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率，分別為0 . 448 %和22 . 4 % 。
  12. In this study, we attempted to construct an engineering strain producing only avermectin bl through the replacement of dna encoding dh2 - kr2 domains of the avermectin pks ( avedh2 - kr2 ) with dna encoding dh2 - kr2 domains from the pikromycin pks in s. avermitilis olm73 - 12, producing only avermectins b and no oligomycin. gene replacement vector pxl201 ( pkc1139 : : 5 ' flank + pia : dh2 - kr2 + 3 ' flank ) was used to transform 5. avermitilis olm73 - 12 protoplasts

    我們以不產寡黴素而僅產阿維菌素b的工程菌olm73 - 12為出發菌株，用委內瑞拉鏈黴菌（ streptomycesvenezuelae ）中編碼pikromycinpks模塊2上完全活性的dh和酮基還原酶（ kr ）的dna區域對olm73 - 12染色體上編碼阿維菌素pks模塊2中dh和kr的區域進行取代，試圖構建僅產b1組分的基因工程菌。
  13. Phaenerochaete chrysosporiun produces extracellular peroxidase system mainly consisting of lignin peroxidase and mn - dependent peroxidase, which has peticuliar mechanism of enzyme for degradation of many organic pollutants

    摘要黃孢原毛平革菌由於其所產胞外過氧化物酶系（主要由木素過氧化物酶和錳過氧化物酶組成）的獨特酶降解機理，能降解多種有機污染物，在環境工程中有著巨大的應用前景。
  14. The paper studied three aspects of extracelluar enzymes in sediments of the tidal flat wetland, namely 1 ) the distibution of five sorts of extracellular enzymes in sediments in the east end of chongming island along the elevation gradient or community succession series, the relationships between the activities of enzymes and the ecological factors, and functions of extracellular enzymes in the process of community succession ; 2 ) the effects of the heavy metal ions and edta on the activity of alkaline phosphatase in sediments of the east end of chongming island by adding and removing of heavy metal ions, discussing whether the activities of extracellular enzymes could be taken as the indicators for the environmental status ; 3 ) the variations of the activities of extracellular enzymes in sediments in the east end of hengsha island after the discarding clay

    本文以長江口典型濕地?崇明東灘為例，首次研究了沿高程梯度或沿植被演替系列沉積物中堿性磷酸酶等五種胞外酶活性的空間分佈規律，分析了胞外酶活性與環境因子的相互關系及其產生機制，討論了胞外酶活性在濕地植被演替中的作用。同時以崇明東灘沉積物為對象，運用重金屬離子的添加和去除等方法，研究了重金屬離子對沉積物中堿性磷酸酶活性的影響，利用胞外酶活性的變化探討了崇明東灘重金屬污染的狀況。此外，本文還研究了橫沙東灘吹泥試驗工程對沉積物環境因子和胞外酶活性的影響並進行了對比分析。
  15. Construction of a double functional recombinant strainof pichia pastoris co - expressing phytase and mannanase and the enzymatic analyses

    植酸酶和甘露聚糖酶雙功能畢赤酵母工程菌的構建和產酶分析
  16. In this study, a new gene c / wew, encoding cholesterol oxidase, was isolated from rhodococcus equi. 4 - 2g2 found in china, which may be useful in clinical diagnosis healthy food and pest management in agriculture. in addition, the gene has been expressed successfully, the expression product has cholesterol oxidase activity, thus this work provided theoretical basis to the development of genetic engineering of cholesterol oxidase

    本研究從我國自行分離的馬紅球菌4 ? 2g2菌株中分離到一種編碼膽固醇氧化酶的新基因choew ，它將可應用於臨床檢測、保健食品和農業抗蟲等領域；同時利用原核表達載體成功的在大腸桿菌中表達了目的基因，表現出膽固醇氧化酶酶活，為膽固醇氧化酶的基因工程利用開發作了一定的理論實驗基礎研究。
  17. In order to break down the rate - limited steps in the artemisinin biosynthesis to improve the artemisinin production and realize the industrial production of artemisinin, related key genes in artemisinin biosynthesis must be cloned and the regulatory patterns of key genes should be studied. for this purpose molecular cloning of related key genes in artemisinin biosynthesis was performed in this thesis work

    利用現代分子生物學和基因工程技術手段，克隆青蒿素生成途徑的關鍵酶基因，研究關鍵酶基因對青蒿素生物合成的調控規律，是打破青蒿素生物合成的限速步驟，大幅度提高青蒿素含量，最終達到利用植物生物技術工業化生產青蒿素的目的必須解決的關鍵問題。
  18. Fusion protein gst - hdt was purified by gstrap affinity chromatography

    將工程菌株發酵所得酶液，經gstrap親和層析，得到融合蛋白gst - hdt純品。
  19. This paper reviewed and discussed the application and advances of fermentation engineering, enzyme engineering, cell engineering and gene engineering in flavor and fragrance industry

    本文闡述了發酵工程、酶工程、細胞工程和基因工程在香料中的應用，並控討了生物技術在香料中的應用前景。
  20. Acetylornithine deacetylase is the key enzyme of producting l - methionine. we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase. in order to get high expression deacetylase strain, we obtain the gene by pcr arge gene. the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr. then taking the nucleotide sequencing compared with the sequence at blast of u. s. a. we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g

    為了獲得高效表達的脫乙酰鳥氨酸酶工程菌株，在工程菌技術改造及其固定化研究做了進一步的研究和探討。我們採用基因工程技術，通過pcr技術擴增出了酰化酶關鍵酶基因?脫乙酰鳥氨酸酶基因arge ，將其克隆到puc19載體中，經酶切鑒定、 pcr鑒定篩選出重組陽性質粒，並測序鑒定，通過美國blast程序進行了基因數據庫相似性比較分析。
分享友人
分享到 Line

分享到臉書