酶底物 的英文怎麼說
中文拼音 [dewù]
酶底物
英文
enzyme substrate-
Comprehensive cellular responses was found in human amnion fl cells following exposure to low concentration of mnng, such as the lowering of dna replication fidelity resulted from alteration of dna polymerase profile ; activation of a lot of transcription factors, such as api, creb, nf - kb etc ; clustering of egfr ( epidermal growth factor receptor ) and tnfr ( tumor necrosis factor receptor ) and activation of camp - pka - creb and jnk / sapk signal pathways
我們發現,低劑量mnng處理后的人羊膜fl細胞有廣泛的細胞反應,並有多個信號轉導通路的激活和基因表達的改變。例如dna復制保真度下降, dna聚合酶譜發生改變,應用報告基因技術和底物磷酸化檢出技術證明細胞一系列轉錄因子如ap1 、 creb 、 nf b等被激活,細胞表面受體如表皮生長因子受體、腫瘤壞死因子受體發生聚簇,細胞信號轉導通路camp - pka - creb和jnk sapk被激活。According to the research of physiological and biochemical indicators or index, components of soluble proteins, substrate protein of phosphorylation and the activity of protein kinase in low - temperature stress in the leaves of brassica oleracea l., we tried to find the law of the physiological and biochemical response of brassica oleracea l. leaf to low temperature. at the same time, discussion on the signal transduction can also provide further evidences for revealing the mechanism of low - temperature stress. the results are showed as follows : malondialdehyde ( mda ), superoxide dismutase ( sod ), ascorbate peroxidase ( asp ) and peroxidase ( pod ) activities were changed greatly after 0 ~ 30min ' s treating with low temperature
本文以甘藍葉片為材料,通過對低溫5脅迫下甘藍生理生化指標、可溶性蛋白組分以及磷酸化底物蛋白、蛋白激酶活性的研究,以期找出甘藍葉片對低溫脅迫的生理生化響應規律,為甘藍露地越冬栽培防範寒害提供理論指導,同時對低溫脅迫下甘藍逆境信號傳導進行了探討,從而為徹底弄清低溫脅迫機理提供進一步的證據,研究的主要結果如下:丙二醛含量( mda ) 、超氧化物歧化酶( sod ) 、抗壞血酸過氧化物酶( asp )和過氧化物酶( pod )活性在低溫處理0 30min發生顯著變化,低溫處理3min后,甘藍葉片內mda含量基本沒有變化,處理5min時出現第一個峰值,達到對照的104 . 10 , 10min出現低谷,僅為對照的86 . 27 ,隨后再次上升, 30min時超過第一峰值,為對照的113 . 93 。Most enzyme assays monitor disappearance of a product.
大多數酶的測量法是檢測其底物的減少或產物的出現。The fluorescence of substrate immediate influences the sensitivity of fluoroimmunoassay. 3, 3 ', 5, 5 ' - tetramethylbenzidine and 2 - chloro - 10 - [ 3 - ( 4 - methyl - l - piperazinyl ) propyl ] - loh - phenothiazine ( prochlorperazine ) are used for the substrate of immunoassay. 3 ) a novel enzyme - link fluoroimmunoassay system using 3, 3 ', 5, 5 ' - tetramethyl - benzidine as substrate to determine the complement 3 ( c3 ) was developed
酶聯熒光免疫體系中的關鍵部分在於生物活性物質的固定和熒光底物的選擇,本文試發展幾種新的熒光底物用於酶聯熒光免疫體系的檢測。Such as when the substrates was benzidine and a - naphthol, the absortion peak was 450nm after catalyticed by dna, and 501nm after catalyticed by pod, if changing substrate to benzidine and pyrocatechol, the absortion peak was 503nm after catalyticed by dna and 603nm after catalyticed by pod. the second difference was the different result after hplc : the result of hplc ( substrates : benzidine and a - naphthol ) showed that the products under the affect of dna and pod had different value on the peak and the area
然後對以聯苯胺與-萘酚為底物,以h _ 2o _ 2為氧化劑,進行高效液相色譜分析,分析結果表明dna與過氧化物酶作用后的產物在峰面積和峰高上有不同數值;同時,不同形式的dna對該反應都有催化活性,表明只要是生物來源的dna (非特異性dna )都具有一定程度的催化活性。Carboxylase an enzyme that catalyzes the incorporation of carbon dioxide into a substrate molecule
羧化酶:一類催化二氧化碳合成底物分子的酶。The study of the ester deriviatives of florescent substances used as the substrate of cholinesterase in human blood
血膽堿酯酶底物的研究The end product inhibitor has no structural similarity to the substrate of the enzyme it is regulating.
最終產物抑制劑與它調節的酶的底物在結構上無相似之處。The molecules that are acted upon by enzymes are termed substrates.
被酶作用的分子稱為底物。In this research, the plant esterase of wheat and - ethanoic acid naphthalene ester were taken as materials to inspect the relation between the wheat esterase and the organic phosphorus, ten linearity equations were established which expressed the esterase vigor and the organic phosphorus density logarithm with the change of the numerical value of ultraviolet resorption, and the monotony range and the extremum of the function were confirmed
摘要本研究以小麥為植物酯酶的酶源, -乙酸萘酯為底物,考察了小麥酯酶與有機磷農藥之間作用的關系,建立了10個以吸光值變化表示的酶活性與有機磷農藥濃度對數之間的線性方程,並確定了函數的單調區間和極值。Isomerase an enzyme that catalyzed the conversion of molecule from one isomeric form to another
異構酶:是能夠催化底物分子進行結構轉化的酶。Ligase an enzyme that catalyzes the bond formation between two substrates at the expense of the breakdown of atp or some other nucleotide triphosphate
連接酶:可將兩個底物結合在一起的酶,此過程需要atp或其他核苷三磷酸供能。This experiment passing to grope for the carbon source constitutes of the culture medium and using t. reesei rut c - 30 induced the expression of # - mannanase ( # - 1, 4 - mannan mannohydrolase ec 3. 2. 1. 78 ). in this experiment i put the constant carbon source ( lactose and locust bean gum ) in the foundation culture medium ( mandels nourishment liquid ) of t. reesei rut c - 30, then proceeded the variable carbon source ( dragon spruce fiber, com rush pith fiber, wheat straw fiber, wheat straw xylan, corn rush pith xylan, dragon spruce mannan ) to single factor, double factor, three factor, four factor and five factor orthogonal experiment. 1 determined the activity of p - mannanase using locost bean gum as substract by the 3, 5 - dinitosalicylic acid method, and observed the growing situation of the gernic at the end i selected the directions for the inducement expression of the # ? mannanase from trichoderma reesei rut - c30 that contained the dragon spruce fiber, wheat straw xylan, dragon spruce mannan
在里氏木霉rutc - 30的基礎培養基( mandels營養液)中加入固定碳源乳糖和槐豆膠,然後將可變碳源(雲杉纖維、玉米芯纖維、麥桿纖維、麥桿木聚糖、玉米芯木聚糖、雲杉甘露聚糖)進行單因子、雙因子、三因子、四因子、五因子的里氏木霉rutc - 30正交培養實驗,並以槐豆膠為底物用3 , 5二硝基水楊酸法測定培養液中?甘露聚糖酶的活力。從而確定了酶活最高且菌體生長良好的含雲杉纖維、麥桿木聚糖和雲杉甘露聚糖的誘導培養基為最佳培養基,用該培養基培養的里氏木霉( t . reesei ) rutc - 30使其轉錄的-甘露聚糖酶( - 1 , 4 - mannanmannohydrolaseec3 . 2 . 1 . 78 ) mrna量能夠滿足rt - pcr的要求。A covalent enzyme - substrate intermediate with saccharide distortion in a mutant t4 lysozyme
在一個群從變型t4溶菌酶中的一個帶有糖變形的共價酶底物中間物。Substrate kinetics of polyamine oxidase from vigna unguiculata
豇豆多胺氧化酶的底物動力學研究Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus
本研究利用線蟲誘導下owvt - 1菌株液體發酵,通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶,生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用,同時測定了該酶理化特性,酶活力在75附近酶活力最高,隨著ph的增加酶的穩定性升高,與膽堿酯酶具有相似的ph曲線,對特異性底物aape ( suc - ala - ala - pro - glu - pna )具有作用, ssi和ci - 2抑制該酶的活性。That shows there are some difference in the steroid regulation between the anuras and the urodeles. among three of the steroid, one can convert to the other, and some enzyme activity is concerned with the conversion
三種類固醇激素存在相互轉化的關系,睪酮可能在雌二醇和孕酮的轉化中起著底物的作用,這種轉化與某種芳香化酶的活性有關。Vanadate, a potent ptpase inhibitor, locks adipocyte differentiation at an early stage in the program and leds to the accumulation of p - c - crkii, a phosphotyrosyl protein that is a substrate for ptpase ha2
研究發現一種強烈的磷酸酯酶抑制劑- -釩酸鈉能夠作用於脂肪細胞的分化早期過程從而抑制其分化,並使一種ptpha2的底物即酪氨酸磷酸化的蛋白質p - c - crkii累積。Inducible enzyme ( adaptive enzyme ) an enzyme that is produced by a cell only in the presence of high concentrations of its substrate or a structurally similar substance
誘導酶(適應酶) :是指細胞內當高濃度酶底物或底物類似物存在時才能產生的酶。Recently, there has been rapid development in the research and application of biosensor, which can detect analytes selectively using the specific reaction of bioactive materials such as the enzyme - substrate, enzyme - coenzyme, antigen - antibody, incretion - acceptor and so on
近年來,生物傳感器的研究和應用發展迅猛,它利用生物活性物質的親和性,如酶-底物、酶-輔基、抗原-抗體、激素-受體等的分子識別功能,可以有選擇地檢測待測物。分享友人