酶性發酵 的英文怎麼說
中文拼音 [xìngfājiào]
酶性發酵
英文
enzymosis-
A bacteriocin with a broad spectrum activity produced by grapevine crown gall biocontrol strain e
澱粉酶產生菌的分離鑒定及發酵條件與酶性質研究Arginine feeding experiment showed that nitrogen metabolism in the s. tenebraius was obviously affected by arginine through two possible ways : ( l ) pronase activity in vitro could be influnced by arginine, as a result, the catabolism of nitrogen - containing macro - molecule was promoted and the nitrogen element in the broth was increased. ( 2 ) arginine could be transformed into glutamic acid, so that the biosynthesis of apramycin was promoted
因而我們認為gln可能是安普黴素生物合成氮元素的供體。 arg添加實驗結果表明, arg可能通過兩種途徑影響黑暗鏈黴菌體內的氮代謝: ( 1 ) arg可能影響胞外蛋白酶的活性,進而促進含氮大分子物質的分解代謝,補充發酵過程中的氮素來源。Effect of agitation rate on batch alkaline pectate lyases fermentation by bacillus subtilis
分批發酵堿性果膠裂解酶的影響Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus
本研究利用線蟲誘導下owvt - 1菌株液體發酵,通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶,生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用,同時測定了該酶理化特性,酶活力在75附近酶活力最高,隨著ph的增加酶的穩定性升高,與膽堿酯酶具有相似的ph曲線,對特異性底物aape ( suc - ala - ala - pro - glu - pna )具有作用, ssi和ci - 2抑制該酶的活性。A water soluble crude polysaccharide in the cells has been isolated from the zymogen thread of hericium erinaceus pers. after the crude polysaccharide has been deamylumized by the combined methods of enzyme and sevage, the four graded component - hpa, hpb, hpc, hpd - would be abstracted by the method of ethanol grading
從猴頭菌( hericiumerinaceuspers )發酵菌絲體中提取出胞內水溶性粗多糖,經酶法和sevage法聯合脫蛋白后,用乙醇分級法分離出hpa 、 hpb 、 hpc 、 hpd四種級分。Studies on liquid - state fermentation for and properties of cellulase from chaetomium thermophlie
液體發酵產生纖維素酶及酶學性質的研究Along with the development of the cytobiology and the molecular biology, and thoroughly research of the biophysics, the biochemistry, the genetics and immunology, it has cultivated the modem biological technology, such al genetic engineering, cellular engineering, enzyme engineering, fermentation engineering and so on, to change biology characteristic to carry on the material transformation, has formed the front biological examination technology : the dna probe, the pcr technology, the molecular mark, the bioluminescence technology, genechip technology and so on the widespread application of these advanced biotechnologies in dairy industry baa impelled the dairying technical transformation, and has been having vital significance to dairy production, research and dairy product security
摘要隨著細胞生物學和分子生物學的發展及對生物物理、生物化學、遺傳學和免疫學研究的深入,培育了基因工程、細胞工程、酶工程、發酵工程等改變生物特性進行物質轉化的現代生物技術,形成了dna探針、 pcr技術、分子標記、生物熒光技術、基因晶元技術等前沿性的生物檢測技術,其在乳品工業中的廣泛應用,推動了乳業的技術變革,對乳品生產、研究和乳品安全意義重大。Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution
3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵,用緩沖液抽提后,經硫酸按沉澱, deae一纖維素離子交換層析,聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶,用sds一page檢測為一條均一譜帶,其分子量約為78kd 。Was supplied. its essential characteristics are as follows : short rods, 2. 0x1. 6 ^ m in diameter, arranged singly, gram negative, facultatively anaerobic, having both a respiratory and a fermentative type of metabolism, oxidase negative and catalase positive
通過形態觀察及生理生化特徵測定發現菌株w12為革蘭氏陰性菌,短桿狀,對數生長期細胞大小為: 2 . 0 1 . 6 m ,單個排列;兼性厭氧,氧化酶陰性,發酵葡萄糖產酸產氣,不運動。Specific pichia clony pcr product showed that foreign phytase gene was integrated into the host cell. the experimental results from flask fermentation and phytase activity assay indicated that phytase gene was effectively expressed by the recombinant pichia
挑選轉化子經過bmgy搖瓶培養、 bmmy誘導發酵后,用釩鋁酸按法測定了表達產物的酶活性,結果表明重組菌株可有效表達具有生物學活性的植酸酶。The milk - clotting mechanism of glutinous rice wine was studied. the results showed that milk - clotting was caused by the protease which was secreted by the microorganism in the glutinous rice wine
摘要研究了江米酒製作米酒奶的凝乳機理。試驗結果證實,江米酒中的凝乳活性因子,即江米酒發酵過程中微生物產生的蛋白酶是米酒奶凝乳的主要原因。As starter cultures, twelve strains of bacillus were screened for the manufacture of long - ripened douchiba from 40 strains isolated from naturally fermented douchiba samples at consecutive stages from qianxi and dafang of guizhou province, through comprehensive evaluation including extracellular proteolytic activity and lipidolytic activity, salt tolerance, ultraviolet light resistance, and antibiotic sensitivity
摘要從貴州黔西和大方兩地傳統陳窖豆豉粑不同工藝階段分離到的40株菌株,經胞外蛋白質和脂肪水解酶活力、耐鹽性能、抗紫外光照射和抗生素敏感性等綜合性能評價,篩選到12株芽胞桿菌可作為陳窖豆豉粑純菌發酵的選擇菌株。Enterobacteriaceae : micro - organisms which ferment glucose and show a negative oxidase reaction when the tests are carried out in accordance with this li
腸道桿菌:依照本li試驗時,能發酵葡萄糖,氧化酶陰性的微生物即為腸道桿菌。When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum
莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎,同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達,所構建的重組質粒ptga ,其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 , 5 、 4 、 2 . 3倍,且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中,搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率,分別為0 . 448 %和22 . 4 % 。This paper describes several latest industrial microbial technologies in detail, which are the synthesis of the chiral diols by epoxide hydrolase from microbie, cofactors regeneration for redox with fdh, production of nano / micro wire by the phage display, metabolic network rebuilding for conventional fermentation and the application of the organic solvent tolerance and the metagenomics technology
本文綜述了幾項最新的工業微生物技術,主要包括:微生物環氧化水解酶催化合成手性二醇、微生物甲酸脫氫酶用於再生氧化還原反應的輔因子、通過噬菌體展示技術得到納米級金屬絲、代謝網路改造和重建用於傳統發酵生產以及有機溶劑耐受菌和宏基因組技術的應用。The effects of direct water extraction, enzymatic hydrolysis and biological fermentation on the yield of water - soluble dietary fiber from rice bran were studied
摘要研究了直接水浸提法、酶解法、生物發酵法對米糠水溶性膳食纖維得率的影響。The results indicated that the yields of water - soluble dietary fiber from rice bran by direct water extraction, enzymatic hydrolysis and biological fermentation were 10. 78 %, 16. 32 % and 10. 28 % respectively, and the method of enzymatic hydrolysis was more suitable for preparation of water - soluble dietary fiber
結果表明:直接水浸提法、酶解法和生物發酵法水溶性膳食纖維得率分別為10 . 78 % 、 16 . 32 %和10 . 28 % ,酶解法更適合制備水溶性膳食纖維。The enzyme activity in fermentation liquid could be inhibited by pmsf and dfp. the fermentation liquor also showed good dehairing activity. the alkaline protease ( named dhap, dehairing alkaline protease ) in the fermentation liquid was purified with hydrophobic interaction chromatography, ion exchange and gel filtration
通過cm - sepharosefastflow離子交換層析, deae - sepharosefastflow離子交換層析, sephacryls - 100 , sephacryls - 200凝膠過濾層析,疏水層析等純化步驟對短小芽孢桿菌發酵液中的堿性蛋白酶進行了純化。Although sds - page displayed that there were several protein bands in the sample after 16h fermentation, enzymatic active stain demonstrated that there was only one band during the whole fermentation period, indicating that there is only one kind alkaline protease in the fermentation liquid
對發酵液進行sds - page和蛋白酶的活性染色分析表明,發酵液中的蛋白質種類不多,且只含有一種堿性蛋白酶。該發酵液所含蛋白酶活性能夠被絲氨酸蛋白酶抑制劑pmsf , dfp完全抑制。The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively
三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變, pcr產物與ppic3 . 5k連接,轉化大腸桿菌,獲得的混合質粒電轉化畢赤酵母gs115 , omm平板篩選適于低溫或對熱穩定的重組子,篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ,其最活反應溫度為45 ,比野生型高出5 ; 40處理30min殘留活性為56 ,大大高於野生型的6 ;初始ph7 . 3528條件下培養72h ,發酵上清酶活為325u / ml 。分享友人