酵母氨酸 的英文怎麼說
中文拼音 [jiàomǔānsuān]
酵母氨酸
英文
saccgaropine-
Based on this, through a lot of yeast, e - polylysine ferment experiments and analyzing the data, comprehend the universality rule of animalcule ferment ; advanced soft - measure model, estimate the parameter non - measurable online, including the parse model based main - regression analyze, the ann model based ann arithmetic. guide the fed - batch control and environment parameter by the optimized track. to advance the last gain, identify the fermentation phase
在此基礎上,經多次酵母發酵實驗、聚賴氨酸發酵實驗及對大量實驗數據的分析,深入了解了微生物發酵的一般性規律;建立了微生物發酵過程的軟測量模型,對不可在線測量的生物參數進行估計,包括由主元回歸分析得到的解析式模型,由神經網路演算法得到的神經網路模型。Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris. by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides, a recombinant expression plasmid ppic9k - fl was constructed, and integrated into the alcohol oxidase region of the host genome
為了提高外源基因的表達量,我們根據畢赤氏酵母偏愛密碼子人工合成了編碼fl胞外區156個氨基酸的cdna序列,目的序列被定向克隆到酵母分泌型表達載體ppic9k質粒上,構建ppic9k - fl表達質粒。The results suggested that the enzymatic activity of invertase from high to low was yh 、 yn 、 yf, and the amino acid sequence of the three strains were completely identical
得出3株高糖酵母在不同生長階段的蔗糖轉換酶酶活由高到低依次為yh 、 yn 、 yf ,而其蔗糖轉換酶的氨基酸序列卻完全相同。Isolation and identification of rhodosporidium toruloides r1 degrading n - acyl homoserine lactone
酰基高絲氨酸內酯酵母菌菌株的分離鑒定及其降解特性Homology analysis blast analysis showed that the amino acid sequence of the asf gene cloned from cotton was significantly homologous with adp - ribosylation factor ( asf ) of mammalian, plant and yeast, etc. the amino acid sequence of the gene shows 99 % ( 179 / 180 ), 98 % ( 177 / 180 ), 96 % ( 174 / 180 ) and 92 % ( 168 / 180 ) identity to arabidopsisthalianaarfl, triticum aestivum, solatium tuberosum and bos taurus, respectively
棉花arf基因的同源性分析應用ncbi進行dna序列的相似性分析,結果表明:棉花arf基因與其它植物、動物和酵母的arf基因具有較高的同源性。棉花arf基因編碼的氨基酸序列與擬南芥,小麥、馬鈴薯、牛的同源性分別達到99 ( 179 / 180 ) 、 98 ( 177 180 ) 。 96 ( 174 180 )楊花腸p核符吞fi曰手谷回伽皿的夭險和92 ( 168ill ) 。Asqs is 70 %, 77 %, 44 % and 39 % identical to squalene synthases from arabidopsis thaliana, tobacco, human and yeast, respectively. the asqs genomic dna has a complex organization containing 14 exons and 13 introns
青蒿鯊烯合酶氨基酸序列與擬南芥、煙草、人類、酵母鯊烯合酶的一致性分別為70 、 77 、 44 、 39 。Chinese herbal medicine, composite multi - dimensional, lysine, copper sulfate, ferrous sulfate, zinc sulfate, manganese sulfate, calcium hydrogen sulfate, yeast powder, etc
中草藥、復合多維、賴氨酸、硫酸銅、硫酸亞鐵、硫酸鋅、硫酸錳、硫酸氫鈣、酵母粉等。After linerization, the recombinant plasmid was transformed into pic hia pastoris by electroporation, which then were cultured in md plate free of histidine, from which the positive colones were propagated
重組質粒線性化后,用電擊法將重組質粒轉化入巴氏畢赤酵母,在缺組氨酸的md板上篩選陽性菌落,然後用不同濃度的g418 ? ypd板篩選多拷貝插入單菌落。The experimental results suggest that the cell disruption ratio can reach 97. 88 % using enzymolysis and nanometer - crushing machine, and the contents of amino acid in the extractives are also considerably raised
試驗結果表明,酵母細胞先經酶解再結合納米破碎機破碎,可使酵母細胞壁的破碎率達到97 . 88 % ,大幅度提高了抽提物中氨基酸的含量。5 - enolpyruvlshimimate - 3 - phosphate synthase ( epsp synthase ) is a necessary enzyme of the shikimate pathway in prokaryote, yeasts, fungi, apicomplexan parasites, plants and algae
原核生物、酵母、真菌、頂配位寄生蟲、植物和藻類中的epsp合成酶是芥草酸途徑中的一個氨基酸必需酶。The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后,轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌,進一步用遺傳毒素g418篩選多拷貝的轉化菌株,命名為gh1 ;將gh1甲醇酵母菌用0 . 5的甲醇誘導表達,發酵上清經90飽和度的( nh _ 4 ) _ 2so _ 4沉澱, yw - 3 ( mwc03000 )的超濾膜超濾,再經cm陽離子交換, c _ ( 18 )反相hplc純化得到分子量為4kd左右的組分,其中4289 . 05的組分經質譜鑒定,氨基酸組成分析和序列測定為正確的表達產物,生物學活性表明其活性為天然毒素活性70 % ,表達量為80mg / l 。We found that there are two types of map1lc3 in rat, mouse and human, besides that, there is another type ( map1lc3c ) in human. we expressed all map1lc3 in hek293 cells respectively and found that the post translational modification of all map1lc3 is similar to yeast apg8 and rat map1lc3 identified except human lc3b. characteristic carboxyl cleavage occurred in conserved glycine of them
在hek293細胞中分別表達這三個物種中的所有的a型、 b型以及人map1lc3c分子,發現除了人的map1lc3b外其它同源物的修飾方式與已知的酵母apg8和大鼠的lc3修飾方式相類似,均發生了羧基端的切割反應而且羧基端保守的甘氨酸是它們發生翻譯后修飾的活性位點。In this article, the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression. adopted a new strategy, multiple copy gene is ligated in the same direction. and then it is cloned into expression vector plasmid ppic9k
本文根據genebank登錄的氨基酸序列,同時考慮構建和表達的需要,化學合成了misgurin基因和接頭,採用一種新的策略,在體外將基因多拷貝同向串連,並將其克隆于表達質粒ppic9k中,通過鑒定並測序正確后,電轉化真核表達宿主? ?畢赤酵母菌株gs115 ,通過營養缺陷型培養基篩選重組子,再利用g418抗性篩選出整合有多拷貝外源基因的重組子。The activity of the invertase of three high - sugar saccharomyces cerevisiae strains were investigated and their amino acid sequence were determined by the use of modern biotech including enzymatic activity determination, specific pcr, the cloning sequencing and bio - information analysis etc
摘要採用現代生物技術中的酶活測定、特異pcr 、克隆測序和生物信息分析等對比研究3株高糖酵母蔗糖轉換酶活性及其氨基酸序列。So it is important to reveal the mechanism of uptake and release of copper in hepatocyte, we aimed to purify a novel copper binding protein from rat liver lysosomal fraction. researches show that copper binding proteins have conserved amino acid sequences - mxcxxc ( x is any amino acid ), can bind with copper. for example atp7b has five mxcxxc sequence, and it was determined 1 mol atp7b can interact 5 mol of copper ions
Irene等人的研究表明鋼結合蛋白具有高度同源性的鋼結合氨基酸序列,從酵母等低等動物到真核細胞n末端都有mxcxxcx代表任意氨基酸廠是鋼結合的區域,例如ai 」 ob的n末端就有5個mxc狀c結構,實驗也證明一摩爾的蛋白質可與5摩爾的硫酸銅結合。To improve the production and activity of the enzyme, the hydantoin hydrolase gene ( hyuh ) was cloned into e. coli m15 and bl21 ( de3 ) by using vector pqe60 and pt221 respectively. two expression plasmids were thus constructed
用pcr技術從質粒puc18 - 169中擴增出l -乙內酰脲水解酶基因( hyuh )和n -氨甲酰基氨基酸水解酶基因( hyuc ) ,分別在大腸桿菌和畢赤酵母中實現了活性表達。We obtained two positive clones after screening. sequencing and blasting results showed that the two clones had the same sequences and encoded part of ked like protein in arabidopsis. the coding region was from n terminal amino acid 240 to c terminus
測序及序列比對結果表明二者序列相同,為ked樣蛋白的部分編碼序列,編碼部分從n端第240位氨宋林夜:利用酵母雙雜交系統篩選與g蛋白及cam相互作用的新型蛋白基酸至c末端,共207個氨基酸。The amino acids sequence of pgl29 is 44 % identical to lst8p from s. cerevisiae and four wd40 domains found in its entire amino acids sequence
在pgl29的編碼蛋白中,共有4個wd40結構域。其全長氨基酸序列與模式生物釀酒酵母中的lst8p有440的問源性。分享友人