酶特異性 的英文怎麼說

中文拼音 [tèyìxìng]
酶特異性 英文
enzymatic specificity
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 特 : Ⅰ形容詞（特殊; 超出一般） particular; special; exceptional; unusual Ⅱ副詞1 （特別） especially; v...
  • 異 : 形容詞1 （有分別; 不相同） different 2 （奇異; 特別） strange; unusual; extraordinary 3 （另外的;...
  • 性 : Ⅰ名詞1 （性格） nature; character; disposition 2 （性能; 性質） property; quality 3 （性別） sex ...
  • 特異性 : distinction
  • 特異 : 1 （特別優異） exceptionally good; excellent; superfine2 （特殊） peculiar; distinctive特異功能 s...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  3. Such as when the substrates was benzidine and a - naphthol, the absortion peak was 450nm after catalyticed by dna, and 501nm after catalyticed by pod, if changing substrate to benzidine and pyrocatechol, the absortion peak was 503nm after catalyticed by dna and 603nm after catalyticed by pod. the second difference was the different result after hplc : the result of hplc ( substrates : benzidine and a - naphthol ) showed that the products under the affect of dna and pod had different value on the peak and the area

    然後對以聯苯胺與-萘酚為底物，以h _ 2o _ 2為氧化劑，進行高效液相色譜分析，分析結果表明dna與過氧化物酶作用后的產物在峰面積和峰高上有不同數值；同時，不同形式的dna對該反應都有催化活性，表明只要是生物來源的dna （非特異性dna ）都具有一定程度的催化活性。
  4. In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank

    本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。
  5. The hatching enzyme from brine shrimp, artemia shrimp, is a pivotal protease which help the encysted embryo escape from its hatching membrane when hatching

    鹵蟲孵化酶（ he ）是由鹵蟲早期胚胎特異性分泌的、在孵化過程中起關鍵作用的一種蛋白酶。
  6. Constructing human colorectal cancer cell line with stably down - regulated grp94 ( 1 ) the plasmid prc / rsv - ribol that contains specific grp94 - targeting ribozyme and the control plasmid prc / rsv were miniprepared, respectively, cleaved by endoenzyme pvuii

    穩定下調grp94的人大腸癌細胞克隆株的構建（ 1 ）分別對含有特異性打靶grp94核酶的質粒prc rsv - ribo1和對照組質粒prc rsv進行小量提取、 pvu酶切鑒定。
  7. The cbd tag of the fusion protein was cut by site - specific protease enterokinase at starting met of the target protein lt 27. it was released from the cbd fusion tag efficiently

    利用融合蛋白中目的蛋白lt 27與cbdtag接頭處的腸激酶特異性識別位點，用腸激酶處理粗純化的融合蛋白，可將卜
  8. The quality of an enzyme immunoassays depends very much on the purity of the antigen or hapten used for conjugation, the specificity of the antibody and the choice of a suitable enzyme label

    酶免疫分析技術的質量依賴于抗原的純度、抗體的特異性、合適標記酶的選用，其靈敏度取決于標記酶的高度純化和高轉化率。
  9. A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv

    以豬瘟兔化弱毒犢牛睪丸細胞毒（ hclv ）中提取的細胞總rna為模板，以rt - pcr技術，擴增出了完整e2基因的cdna片段。經電泳、酶切分析，證實了所擴增片段為e2基因特異性片段。
  10. Effect of generalized unspecifical immunoreaction on activities of some enzymes in rat testis

    全身性非特異性免疫反應對大鼠睪丸幾種酶活性的影響
  11. The template specificities found with dna polymerases from human leucocytes is summarized in table 1.

    人白血病DNA聚合酶的模板特異性簡列于表1中。
  12. The template specificities found with dna polymerases from human leucocytes is summarized in table 1

    人的白血病dna聚合酶的模板特異性簡列于表1 。
  13. In this paper, a field strain of infectious bronchitis virus was isolated from proventriculus tissue, morphological observation by electron - microscope and the biological characterizations of the virus were studied, pairs of specific primers are designed and synthesized in correspondence with them, according to the published sequences of infectious bronchitis virus three structural protein ( spike protein s membrane protein m nucleocapsid protein n ) genes, the cdna of si gene, s2 gene, m gene. n gene of ib v isolate lx4 were amplified by rt - pcr and full sequences were first reported

    在此基礎上，根據國內外已發表的ibv基因序列，分別設計特異性引物，應用不同引物進行反轉錄合成cdna ，分片段對ibv的主要結構基因進行pcr擴增，並分別將各個目的片段克隆到puc19載體上，在大腸桿菌dh5中實現目的基因的分子克隆，經藍白斑篩選、限制性內切酶分析、 pcr鑒定，篩選出重組陽性質粒，並對各個目的基因片段進行序列測定，從而獲得ibv主要結構基因全序列。
  14. Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus

    本研究利用線蟲誘導下owvt - 1菌株液體發酵，通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶，生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用，同時測定了該酶理化特性，酶活力在75附近酶活力最高，隨著ph的增加酶的穩定性升高，與膽堿酯酶具有相似的ph曲線，對特異性底物aape （ suc - ala - ala - pro - glu - pna ）具有作用， ssi和ci - 2抑制該酶的活性。
  15. This modification includes : ( 1 ) selecting two important molecules as candidates, ( 2 ) choosing a promiscuous t - cell epitope, and two b - cell epitopes or conserved amino acid sequences from the two important molecules, ( 3 ) connecting them adequately through analysis by the molecule designing software. therefore, the synthetic new antigen may interfere with the process of fertilization by multiple ways and its contraceptive effects may be enhancing. based on the molecule designing methods, the b - lymphocyte cell epitope of sperm / testis specific protein sp17 and cyritestin which interfere with fertilization in mouse, as well as the promiscuous th cell epitope of the ribonuclease ( rnase ) in bovine were selected

    本研究以蛋白質分子設計的理論和方法研究避孕疫苗，將sp17和cyritestin關鍵表位和牛核糖核酸酶非選擇性th細胞表位合理組合，獲得新抗原- 35肽序列；並在合成、純化後分別與弗氏佐劑、免疫刺激復合物（ iscoms ）混合后免疫不同遺傳背景的雌性小鼠，觀察血清和生殖道內的特異性抗體滴度的動態變化、生育力的改變以及免疫后小鼠重要臟器的組織病理學改變：以及在ivf下，新抗原的特異性抗血清對精卵相互作用的影響及抗原在精子表面的特異性定位。
  16. Based on this consideration, we chose human colorectal cancer cell line ccl229 as the experimental object, down - regulating its grp94 expression by specific ribozyme targeting. we observed its effects on the biological characteristics of ccl229 and upr pathway, and gained further understand of the grp94, upr and tumor relationship

    基於此，我們選擇了人大腸癌細胞株ccl229作為研究對象，通過特異性核酶打靶的方式，下調細胞中grp94的表達水平，觀察其對人大腸癌細胞某些生物學特性及upr通路的影響，加深對grp94 、 upr和腫瘤這三者間關系的了解。
  17. Deduced amino acid sequence of s1, s2, pvin were also highly homologous each other ( 98 %, 99 % in each case ). the stilbene synthase genes were excised from the plasmids by bamh i and sac i digestion and intergrated into a binary vector, pbi121 and pev2, from which the p - glucuronidase ( gus ) gene sequence had been removed by the same digestion to prepare a 35s promoter - stilbene synthase 2 - nopaline synthase polyadenylation site construct and a tfp2 promoter - stilbene synthase 1 - nopaline synthase polyadenylation site construct. the recombinant plasmids were called pbs2, pev2s 1. respectively

    用bamhi和saci同時酶切ps2 （ s2表示來自雷司令的芪合酶基因） 、 ps1 （ s1表示來自粉紅玫瑰的芪合酶基因）以及pbi121 、 pev2 ，使得s2 、 s1分別插入替代pbi121 、 pev2中的gus基因，構建成植物表達載體pbs2 、 pev2s1 ， pbs2中含camv35s組成型啟動子，使s2基因能在番茄植株的各個部位表達； pev2s1則含有果實特異性啟動子tfp2 ，使s1基因只在番茄果實中表達。
  18. Pcr amplification using 2 degenerate primers for nitrogenase fe protein gene was performed with chromosomal dna isolated from the 29 isolates. the result suggested that a nifh amplicon of 323 nucleotides was detected in 7 isolates and the 7 isolates are c4 c5 g1 g2, w5 t1 and t7. these pcr amplified fragments were cloned, and sequenced

    首先利用芽孢桿菌中芽孢的抗熱性將土壤溶液在100沸水中煮10 - 15分鐘，然後用選擇性無氮培養基進行初篩得到29株菌落形態不同的菌株；接著用固氮酶結構基因nifh的特異性引物對這29株菌進行pcr擴增，結果表明其中7個菌株具有nifh基因，這7個菌株的編號依次為： c4 、 c5 、 g1 、 g2 、 w5 、 t1和t7 。
  19. 1. a new method to identify _ amylase activity and its producing bacteria : the blue complex was formed by unspecific adsorption, after mixing starch and trypan _ blue. the adsorption weakened when the starch was hydrolyzed to small molecular by _ amylase, and the trypan _ blue was released inside the hydrolyed zone. the starch around the zone which was not hydrolyzed adsorbed free trypan - blue so that the colour of medium became bluer than that of place in hydrolyzed zone

    快速鑒定並篩選-澱粉酶及其產生菌的新方法：錐蟲藍染料和澱粉由於靜電非特異性吸附結合后使澱粉呈穩定的藍色，當澱粉被澱粉酶水解后因分子變小吸附力減弱，而讓錐蟲藍游離出來，游離的錐蟲藍被周圍未水解的澱粉吸附而使顏色加深，澱粉水解區則形成無色、透明的水解圈。
  20. Integron consists of an integrase gene ( inti ), a integrase specific recombination site ( atti ), and gene cassettes with varied number

    整合子由整合酶（ integrase ）基因（ intl ） 、整合酶特異性重組點atti和數目不定的基因盒（ genecassette ）組成。
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