酶裂解 的英文怎麼說
中文拼音 [lièjiě]
酶裂解
英文
enzymatic lysis-
Effect of agitation rate on batch alkaline pectate lyases fermentation by bacillus subtilis
分批發酵堿性果膠裂解酶的影響The best studied inducible and repressible enzyme in phytopathogenic prokaryotes is endo - pectate lyase in e. carotovora
植物病原原核生物中研究得最清楚的是胡蘿卜軟腐菌的果膠酸內裂解酶。( 3 ) uv - b radiation increased the phenylalanina ammonia - lyase ( pal ) activity in leafy thallus of duckweeds, and as a consequence, flavonoids were accumulated. flavonoids synthesis induced by supplementary uv - b is an adaptive response of duckweeks under enhanced uv - b radition
( 3 )紫萍可以通過提高uv - b吸收物質如類黃酮合成酶苯丙氨酸氨裂解酶( pal )活性,促進類黃酮的積累來適應uv - b輻射的脅迫。This inactive product is cleaved by a converting enzyme, mainly in the lung but also in the kidney and brain, to an octapeptide, angiotensin ii, which is a potent vasoconstrictor that also stimulates release of aldosterone
這一非活性產物經轉換酶裂解,主要是在肺部但也在腎和腦部,成為八肽,即血管緊張素,這是和中強烈的血管收縮素,可刺激醛固酮的釋放。Optimum fermentation condition for producing alginase
海藻酸裂解酶的發酵條件優化Fusion expression of m - centrin in e. coil bl21 was performed by induction of fptg. fusion protein was digested by ppase and was purified by gst chelating affinity chromatography and ion exchange chromatography. the final products were checked by sds - page gel
融合蛋白gst - m - centrin菌體經過超聲波裂解后得到的上清夜經過gst親和層析後用prescissionprotease ( ppase )酶切,酶切產物再次經過gst親和層析和hitrapq陰離子交換層析兩步柱層析純化后,得到純度較高的的m - centrin 。Activity of phenylalanine ammonia - lyase ( pal ) was increased by uv - b radiation, and as a consequence, uv - absorbing compounds were accumulated. it was inferred from our results that the accumulation of uv - absorbing compounds such as flavonoids may be a common response for plants in resistant to enhanced uv - b radiation
在uv - b處理下,紫萍的苯丙氨酸氨基裂解酶( pal )活性上升,並導致其體內吸收uv - b的物質積累,推測類黃酮積累可能是植物抵禦uv - b逆境的一個共同效應。Purification and properties of polygalacturonic acid lyase from ramie degumming bacillus subtilis no. 16a
苎麻脫膠聚半乳糖醛酸裂解酶的純化及酶學性質A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接;用連接產物轉化大腸桿菌jml09感受態細胞,置含amp 、 x - gal和iptg的lb平板上培養12 20小時;挑取白色菌落於選擇性培養基擴大培養,堿裂解法小量提取質粒dna ,並進行酶切分析鑒定,結果獲得整合有prvge基因的重組質粒pugedna ,並與其它prv分離株進行ge基因序列同源性分析。Lyase an enzyme that catalyzes the separation of two parts of a molecule with the formation of a double bond in one of them
裂解酶:一類能催化一個分子的兩部分分離,並在其中一個上形成雙鍵的酶。Hydroperoxides were produced from unsaturated fatty acid in oils catalyzed by lipoxygenase, then in the hydroperoxide lyase, the grassy green and leafy green aroma volatile aldehydes were made
摘要脂肪氧合酶催化油脂中的不飽和脂肪酸生成氫過氧化物,氫過氧化物再在裂解酶的作用下,生成具有青草香、青葉香香氣特徵的揮發性醛類物質。The basic approach of unsaturated fatty acid catalyzed and oxygened by enzyme, the preparation of lipoxygenase and production of hydroperoxides, the preparation of hydroperoxide lyase and cleaving of hydroperoxides were mostly discussed
主要論述了不飽和脂肪酸酶催化氧化的基本途徑,脂肪氧合酶的制備及氫過氧化物的生成,裂解酶的制備及氫過氧化物的裂解過程。Then, nap300 and 75 were fused in a similar way to phba gene encoding 3 - ketothiolase for a direct comparison. the result indicated that the two promoter ' s expression efficiency reached a peak at the same developmental stage of tobacco, which means they have the advantage of being used simultaneously for expressing different foreign genes in plant
將nap300 、 7s分別與phba基因(編碼3 -酮硫裂解酶)相連,在相似表達環境中對二者功能進行比較,發現兩個啟動子表達模式基本相同並在同一時期達到活性高峰,因此nap300可用於改善phb合成基因在植物體內的表達調控。Genome dna of all blood samples were extracted by using erythrocyte lysis solution and pcr buffer / nonionic detergent / proteinase k. a 109bp fragment was amplified from the human genome dna. 2. in 166 han samples, 19 ( 11. 45 % ) heterozygous genotypes of codon 54 mutant allele were found by pcr - sscp
用紅細胞裂解液和pcr緩沖液非離于去污劑蛋白酶k兩種自配試劑成功地提取了所收標本的人白細胞基因組dna ,並以此為模板成功擴增出預期的109hpmbl目的基因片段。4. the expressed result of pbv - a, pbv - b and pbv - c showed that the expressed protein was soluble and no inclusion body was been found. sds - page analysis show the molecular weight of protein expressed by phaa was 42kda which was equal to 3 - ketohilase, the molecular weight of protein expressed by phab was 26kda which was equal to acetoacetyl - coa reductase, and the molecular weight of protein expressed by phac was 63kda which was equal to pha synthase
表達載體pbv - a 、 pbv - b和pbv - c經誘導表達,發現所表達的蛋白均為可溶性蛋白,沒有包涵體出現;蛋白經sds ? page分析表明,基因phaa表達的蛋白分子量為42kda ,與?酮硫裂解酶分子量大小一致;基因phab表達的蛋白分子量為26kda ,與乙酰乙酰coa還原酶分子量大小一致;基因phac表達的蛋白分子量為63kda ,與pha合成酶分子量大小一致。The brine shrimp he was prepared and purified by 67 % ammonium sulfate precipitation, deae - sepharose fast flow ion - exchange chromatography, and sephacryl column gel - filtration, and its biochemical and enzymological properties were identified in this study. it was found that the deduced molecular weight of he in sds - page is about 98. 5 kda, and its proteolytic activity was optimized at ph of 7. 5 - 8. 5 and at temperature of 40, respectively
我們利用67硫酸銨沉澱、 deae - sepharosefastflow陰離子交換柱層析和sephacryl凝膠過濾柱層析,並以酪蛋白為其蛋白酶水解活性的檢測底物、以卵膜為其卵殼裂解活性的特異性底物,從鹵蟲胚胎孵化液中分離純化出了鹵蟲的孵化酶分子,其在sds - page電泳中的分子量約為98 . 5kda 。Protein degradation is one of the major regulatory processes that allow the adaptation, repair, or removal of mutated or damaged thylakoid proteins during environmental or developmental changes. intracellular proteases play an essential role in the turnover of intracellular proteins
在光合作用中,葉綠體類囊體膜蛋白復合物進行光能的吸收、傳遞和轉化,水的裂解及光合磷酸化等,這些膜蛋白的周轉更新需要蛋白酶的作用。A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses
依據apmv - 1融合蛋白( f )基因裂解位點的核苷酸序列與其毒力的相關規律,分別設計合成了四條寡核苷酸引物,建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應( rt - pcr )技術。All study aim is to lay a foundation for clinic appliance. methods : 1 ) hpfl was isolated by enzymatic digestion derived from rat fetal liver on ed13. 5d. furthermore, erythrocyte and other cell were removed from hpfl by erythrocyte - cracking solution and different attachment method
研究方法: 1 )取ed13 . 5d的大鼠胎肝,酶消化法離散細胞,用紅細胞裂解液去除紅細胞,差速貼壁法去除其它細胞,接種于不同的基質和培養液中, mtt法比較不同培養液和培養基質對大鼠hpfl的體外生長影響。We find that the fusion proteins are inclusion bodies when the bacteria lysised by the lysozyme. the product of pet - 32a ( + ) - igf - i was analyzed by western blotting, which confirmed that the hybrid protein was expressed as expected
菌體採用溶菌酶裂解,融合蛋白以包涵體形式存在;對higf -融合蛋白進行了westernblot分析,確認所表達蛋白為目的蛋白。分享友人