重構抗體 的英文怎麼說
中文拼音 [zhònggòukàngtǐ]
重構抗體
英文
reshapedantibody-
The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。The main work is as follows : 1. an error resilient algorithm to collaborate multiple description coding ( mdc ) and error concealment ( ecn ) is presented. the strategy of this method is to reconstruct the low quality image followed by recovering the lost wavelet tree with error concealment method when end - user receives few descriptions
在誤碼環境下，要保證解碼端重構圖象的質量，就要綜合採用多種抗誤碼方法，本文對差錯復原技術的綜合應用進行了研究，具體內容如下: 1 、提出了圖象多描述編碼與差錯隱藏相結合的差錯復原演算法，在接收端接收到的描述較少時，首先根據接收到的若干描述重構低質量的圖象，然後採用差錯隱藏方法恢復丟失的子波樹。Total rna was extracted from the second stage larve of hypoderma sp, then single chain cdna was synthesized by reverse transcription using oligo ( dt ) 18 as a primer. the hypodermin c ( hc ) and hypodermin a ( ha ) gene specific primers were devised by dnastar software
本試驗的目的旨在進行hypodeminc （ hc ）和hypodermina （ ha ）基因的克隆、測序、構建重組表達載體並誘導表達，獲得重組抗原，以解決天然抗原的不足並為診斷和免疫試劑的產業化奠定基礎。Thus, immunologists have sought smaller molecules with the antigen - recognition capability of antibodies. the variable domains of the heavy ( h ) and light ( l ) chains are sufficient for antigen recognition but the non - covalent complex of the two variable domains ( fv ) is unstable. it is possible to genetically engineer a single - chain fv ( scfv ) with the h chain v region connected to the l chain v region via a 15 amino acid linker composed of serine and glycine amino acid residues
二、日本血吸蟲單克隆杭獨特型抗體np30的單鏈狐（ scf ）的構建、表達及對balbic小鼠誘導保護性作用研究l 、日本血吸蟲單克隆杭獨特型抗體np0的單鏈抗體歸cfv ）的構建、表達通過pcr方法體外擴增並經測序驗證的重鏈、輕鏈可變區（ vh 、 vl ）基因先後重組入原核表達質粒ptha90相應的位點上，中間通過一連接肽（ gly在er ） 。Then using ecbp21 antibody and immunogold transmission electron microscopy method, we studied the subcellular localization of ecbp21. the results indicated that the gold particles were mainly localized in the cell wall in callus cells and rachis cells of angelica dahurica. these results indicated that ecbp21 mainly localized in cell wall, which provide a direct evidence of the extracellular existence of ecbp21. furthermore, using ecbp21 antibody and immunohistochemical method, we studied the organic specially distribution of ecbp21, the results indicated that ecbp21 distributed in all organize, but it distributed more in leave n flower rachis than in leafstalk and root
首先，構建了ecbp21表達載體，誘導了重組蛋白的表達，並通過膠回收法獲得了大量純化重組ecbp21蛋白，制備了高效價、高特異性抗體；隨后，利用ecbp21抗體，結合免疫膠體金電鏡定位技術進行了ecbp21亞細胞定位研究，結果顯示：在白芷愈傷組織細胞和花序軸細胞中金顆粒主要分佈在細胞壁區域，而在細胞內未發現或僅有少量金顆粒分佈，表明ecbp21蛋白主要定位於細胞壁區域，這為細胞外cambp （ ecbp21 ）的胞外存在提供了直接證據：進一步，利用ecbp21抗體，通過免疫組織化學分析研究了ecbp21組織特異性分佈狀況，結果表明ecbp21在白芷各組織中均有分佈，但在葉、花、花序軸中分佈較多，而在葉柄、根中分佈較少。The system makes impedance measurements using an array of 32 electrodes on the surface of the body, and reconstructs the images using a veighted backprojection algorithm
該系統採用32體表電極進行阻抗測量，用加權反投影演算法進行圖象重構。This paper, in allusion to damage of expansion joint of bridge, introduces a sort of new structure of jointless device - semi - integral abutment jointless bridge, and establishes model of general structure finite element of this bridge, and in details analyses its qualities of stress. the model considers the interaction of superstructure - substructure - soil. resistance of the soil behind abutment adopts still soil pressure and elastic resistance when the temperature rising, but active soil pressure when dropping. analysis of qualities stress of bridge includes of action of dead load, car load, temperature load of season character. taking an 100m length pc semi - integral abutment jointless bridge as instance, the paper establishes the model of plane frame by applying large common software - anasys, and analyses it by elastane and large deformation to imitate character of stress and deformation of general bridge. at the same time corresponding models of finite element of joint bridge and integral abutment bridge are established, and unique stress and performance of structure of semi - integral abutment jointless bridge are researched by comparing and analysing character of three different models under same loading, and controlling factor of design and constitution are generalized. in conclusion, for semi - integral abutment jointless bridge, on one hand shear, moment and deflection of the main beam and axis force of substructure are approximate with that of joint bridge, and integral stifmess of structure is increased and damage of expansion joint is eliminated, on the other hand there is axis force in main beam, shear and moment of substructure are bigger than that of joint bridge. in all this type of jointless bridge has rational stress and is worth of application and popularization
半整體式橋臺無縫橋梁計算模型考慮了上部結構-下部結構-土的共同作用；臺後土抗力升溫時採用靜止土壓力加彈性土抗力，降溫時採用主動土壓力；分析橋梁受力性能時考慮了結構自重、車輛荷載和季節性溫變荷載的作用。以一座橋長100mpc半整體式橋臺無縫橋梁為實例，採用大型通用軟體ansys建立平面框架模型，進行彈性、大變形分析，模擬全橋受力和變形特點；同時建立與此對應的有伸縮縫橋梁和整體式橋臺無縫橋梁有限元模型，通過三種橋型同步加載對比分析，研究半整體式橋臺無縫橋梁獨特的受力特點和結構性能，總結其設計控制因素和構造要求。通過本文研究知：半整體式橋臺無縫橋梁主梁的剪力、彎矩、撓度和下部結構的軸力與梁式橋相近，結構整體剛度增大，併除去了伸縮縫病害；但主梁增加了軸力，下部結構剪力、彎矩較梁式橋大；綜合分析是一種受力合理有實踐和推廣價值的橋型。Vp2 is one of viral structural proteins on the surface of provirus. its neutralizing antigenicity has been proved. in this study
Vp2是鵝細小病毒結構蛋白之一，是gpv的重要保護性抗原，在體內誘導的抗體具有中和作用。The pc rigid frame bridge can meet with the carrying capacity of long span bridge. this kind of structure is fit for the comfortable steer with no other expansion joints except for those at the two ends. it characterized the wholeness of structure, good astigmatic ability and load - bearing ability, construction rapidness, bright and pithiness bridge style, easy to maintain and convenient for segmental construction using the cantilever technique, so rigid frame bridge have taken an increasingly great proportion in bridges constructed and those under construction at city and county
剛構橋能滿足特大跨徑橋梁的受力要求，除兩端以外無其他伸縮縫，有利於行車平順舒適，具有結構整體性能好、抗震能力強、承載能力強、施工快捷、橋體簡潔明快、維護方便和便於懸臂施工等結構特點，因而在城鄉已建和在建的橋梁中占據了越來越大的比重。In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer
應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。Significance of some measures to improve the application of computer simulation technology in structural seismic analysis, including establishing or upgrading the hysteresis model and multiparameter failure criteria of structural members, studying the damping mechanism of structures during decomposing, discussing the application of database technology in simulation system, developing analysis models for innovating structures and algorithms for analysis of earthquake responses of structures with initial damage, and introducing parallel technology is pointed out
指出建立或完善構件層次的滯回本構模型和多參數破壞準則，研究結構解體前後的阻尼機制，探討數據庫技術在模擬系統中的應用，發展新型結構體系的分析模型以及具有初始損傷結構的地震反應分析方法，引入并行計算技術等對推動計算機模擬技術在結構抗震分析中的應用具有重要的意義。Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。Sensors using fbg as the sensing component have many advantages including light weight, small size, anti electromagnetism interference, convenience in fiber connecting, wavelength absolute coding etc. multiple point detection of materials ( temperature, strain etc. ) can also be achieved by connecting several sensors into a net using variety of multiplex techniques and embedding it into or sticking on the surface of the material or structure
以光纖布拉格光柵（ fbg ）為傳感元件的傳感器具有重量輕、體積小、抗電磁干擾、易於光纖連接、波長絕對編碼等優點，還能把多個傳感器利用各種復用技術連接成傳感網路，埋入材料和結構內部或貼裝在其表面，實現對其特性（如溫度、應變等）的多點監測。Concrete small hollow block, which is in favor of environment and saving plantation, is a kind of wall material to substitute for clay block
混凝土小型空心砌塊是一種替代粘土磚的良好墻體材料，使用砌塊墻體能節省耕地有利於環保，而且砌塊墻體較磚墻自重輕有利於結構抗震。2. the putative epitopes that displayed on phages were identified by indirect elisa using swine antisera against prrsv and mouse antisera to recombinant structural protein of prrsv. seven putative epitopes could be recognized by antisera
利用prrsvbj - 4陽性血清和鼠源抗重組結構蛋白抗體，採用間接elisa方法對噬菌體展示的表位進行了鑒定。4. research of the testing system for bit and the reconstruction of impedance imaging a hardware system for eit technique was made in this paper and the physical model with 16 electrodes was obtained. real time collection, process for data and imaging reconstruction are done by a computer
研製了實時eit硬體系統並對物理模型進行了實際測試和阻抗圖像重構研製了eit硬體系統，建立了16電極的物理模型，並利用計算機進行實時數據采沁狂十eit技術的腦內電特性與功能成像研究集、處理與圖像重構。However, due to masonry structure ' s high self - weight and low aseismic capacity, the total height of the building and the number of storeys are strictly limited
目前，其主要結構形式是底部框剪砌體房屋，但是由於砌體結構自重大，抗震性能差，房屋的層數及高度受到嚴格限制。Under the horizontal earthquake action and wind force, aim at " the pure frame structure with rectangle columns ", " the pure frame structure with special - shaped columns ", " the frame - truss structure with special - shaped columns " and " frame - shear wall structure with special - shaped columns ", earthquake action analysis was done by the spacial finite element method through the changes structural parameter. analyzing systematically " structure vibration mode ", " vibration period ", " structure lateral rigidity ", " seismic action force ", " seismic response force ", " floor seismic shear force ", " lateral horizontal displacement of structure " and " members internal force ". results indicate : ( 1 ) based on equal area, the special - shaped columns replaced the rectangle columns, the structural lateral rigidity enlarges, the lateral displacement minishes obviously, the earthquake response increase slightly, the biggest increasing amount of frame columns axis - compress ratio is smaller than 0. 08. the whole aseismic performance of structure has improved ; ( 2 ) when the section ' s length and section ' s thickness ratio of special - shaped columns is smaller than 3. 6, the structure benefits to resist seismic action ; ( 3 ) the angle of horizontal seismic action with the whole coordinate is 0 degree, structure earthquake response is bigger, belonging to a control factor of structure aseismic design ; ( 4 ) the frame - truss structure with special - shaped columns and the " a " - brace has the biggest lateral rigidity ; ( 5 ) the frame - shear wall structure with special - shaped columns have bigger lateral rigidity and smaller displacement, members internal force enlarged just rightly, have much superiority of resisting seismic action ; ( 6 ) in the higher seismic fortification criterion region ( 8 degree of seismic fortification intensity ), aseismic disadvantageous building site ( iii type site ), adopting special - shaped columns structure system, should reduce possibly the building ' s self - weight in order to reduce the earthquake response ; ( 7 ) the response spectrum method of computing seismic response and the time - history analysis method have similar analysis result
西安理工大學碩士學位論文在水平地震作用下，並考慮風荷載組合，分別對「矩形柱純框架結構」 、 「異型柱純框架結構」 、 「異型柱框一析架結構」及「異型柱框-剪結構」等四種結構體系，通過改變結構參數，運用空間有限元方法，進行地震作用計算。系統地分析研究「結構振型」 、 「振動周期」 、 「結構側向剛度」 、 「結構地震反應力」 、 「樓層地震剪力」 、 「結構側向位移」 、 「層間位移角」 ，以及「構件內力」 。結果表明:在等面積原則下，異型柱代換矩形柱后，結構剛度增大，側向位移明顯減小，地震反應力略有增加，框架柱軸壓比最大增幅小於8 % ，結構整體抗震性能有所提高;當異型柱肢長肢厚比小於等於3 . 6時，結構有利於抗震;水平地震作用力與整體坐標夾角為0度時，結構地震作用效應較大，屬結構設計的控制因素之一;異型柱框一析架結構採用「人」字斜撐，側向剛度大於「八」字斜撐和「人一八」字混合斜撐;異型柱框一剪結構側向剛度大、位移小，構件內力增大適中，是一種抗震性能優越的結構體系:在抗震設防烈度較高地區（ 8度）和抗震不利的建築場地（ m類場地） ，採用異型柱結構體系時，應盡可能減輕結構自重，降低地震作用力;分別採用「振型分解反應譜」法和「時程分析」法進行地震作用計算，兩種方法所得結果基本一致。The patterns of sds - page indicated more than 30 % of recombinant proteins could be obtained from the extract of e. coli bl21. furthermore, library of recombinant phage antibodies was constructed from total rna isolated from spleen of the female balb / c mice immunized by bull sperm
首先用牛精子免疫雌性四周齡balb c小鼠，從其脾臟組織分離總rna ，應用重組噬菌體抗體庫技術，構建了一個針對牛精子的噬菌體抗體文庫。分享友人