野生型菌株 的英文怎麼說

中文拼音 [yěshēngxíngjūnzhū]
野生型菌株 英文
wild-type strain
  • 野 : Ⅰ名詞1 （野外） open country; the open:曠野 open spaces [country]; wilderness; 田野 open fields; ...
  • 生 : Ⅰ動詞1 （生育; 生殖） give birth to; bear 2 （出生） be born 3 （生長） grow 4 （生存; 活） live;...
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 株 : Ⅰ名詞1. （露在地面上樹木的根和莖） root and stem of a tree above the ground 2. （植株） individual plant; plant Ⅱ量詞（棵）
  • 野生 : wild; uncultivated; feral野生動植物 wilding; 野生蜂 wild bee; 野生生物資源保護 wildlife conservat...
  • 菌株 : bacterial strain
  1. In most wild type e. coli strains containing heterologous pha synthase genes, the amount of pha accumulation was almost undetectable while in e. coli km32b the amount of pha accumulation were clearly visible

    在多種野生型大腸桿菌中，盡管使用了各種不同的表達載體，也很難獲得可供檢測的pha ；但應用大腸桿菌的fqdb缺失突變株卻可以獲得較多量的pha 。
  2. In order to study the mechanism of the effect of low concentration tfp on the proliferation of s. pombe, we watch yeast cells loaded with fluo - 3 under laser scanning confocal microscope ( lscm ). the fluorescence intensity reflected the cytosolic free calcium concentration. the result showed that, the cytosolic free ca2 + concentration in s. pombe cultured in ca2 + - free medium was 2 ~ 3 times lower than that in s. pombe cultured in medium containing 10umol / l ca24, while ca2 + concentration in s. pombe treated with 50umol / l tfp was 4 - 5 times higher

    本文發現增加胞外鈣濃度以及低濃度（ 20 100 mol l ）三氟拉嗪（ tfp ）不但能促進野生型s . pombe細胞的增殖，而且對mfp7菌株也有同樣的效應，這說明胞外ca ~ （ 2 + ）和低濃度tfp對不同遺傳型裂殖酵母細胞的增殖均有促進作用。
  3. However it is susceptible to protease ( s ) and cannot express in a large amount in the sporulation phase of natural occurring strain of b. s

    但它在野生型菌株中的表達量低，易被蛋白酶降解失活。
  4. The research interests of this group include : aborvirus diagnosis technology development and the interaction of aborvirus and mosquitoes, entomopathogenic bacteria and insecticidal gene resources, microbial genomics and comparative genomics, insecticidal proteins and their mode of action, construction of engineering strains with higher toxicity and wider active spectrum, production, standardization and the application of bio - pesticide and other microbial agents, resistance mechanism in target insects and the resistance management

    重點研究登革熱病毒、乙型腦炎病毒和西尼羅病毒的快速檢測及病毒與宿主的相互作用關系，蚊蟲病原微生物菌種及其基因資源，微生物基因組學和比較基因組學，殺蚊毒素蛋白特性和作用方式、殺蚊細菌的遺傳改良和工程菌株的構建，新型細菌殺蚊制劑的研製及野生型和重組微生物對環境的安全性評估等，發展新的生物防治技術，建立和完善以生物防治為主的蟲媒病毒媒介蚊蟲綜合防治體系。
  5. Pllz1112 was transformed into m145 to disrupt sc6a9. 34. a mutant named hxy1 was obtained and confirmed to be disrupted at correct site by southern blotting

    將phz1112導入野生型菌株m145中獲得基因中斷菌株hxy1並通過southern雜交驗證hxy1在正確位置發生了基因中斷。
  6. In order to reveal the mechanisms of extreme radioresistance and dna repair in deinococcus radiodurans, we examined proteome changes in a wild type strain following y - irradiation using 2 - dimensional polyacrylamide gel electrophoresis and silver staining

    為了研究耐輻射球菌極強的輻射抗性與dna修復機理，我們應用雙向電泳結合銀染的方法考察了野生型菌株kd8301在射線照射前後細胞體內總蛋白組的變化情況。
  7. The dnd cluster seemed to express when it was carried by the high copy - number plasmid because complementation of individual dnda, dndb or dndc mutation could be observed and such expression did not seem to influence the normal dnd expression in wild type s. lividans 1326

    而在高拷貝質粒上的dnd基因似乎是李愛英變鉛青鏈黴菌dna異常修飾系統的分子生物學研究能表達的，因為它能紅補分別在dn劇、 dndb和咖jc發生單個基因突變的菌株，而且這種表達似乎也不會影響野生型菌株1326中原有的dnd基因簇的表達。
  8. S. lividans mutant strains zx1 ( dnd cluster deleted ) and zx64 ( dnda disrupted ) had pleiotropic mutations including low mel expression and poor sporulation. it was speculated that dnda together with its downstream dna ( 2. 5kb ) might be involved in these two phenotypes because dnda together with its downstream dna could restore normal sporulation and mel expression to zx64, while dndb and dndc had no such effect because lai and la2 showed no obvious difference in these two phenotypes from wild type s. lividans 1326

    另外通過比較這幾個突變株及野生型菌株在產孢和黑色素基因（ mel ）表達方面的差異，推測dnda及其下游區域與變鉛青鏈黴菌的產孢和刺激外源黑色素基因的表達有關，而dndb和dndc則與之無關，因為la1和la2在這兩種表型上與野生型菌株無明顯差異。
  9. Plant assay was performed with the wild - type strain gx201, the mutant strain gx217 and the function recovered strain rgx217. the results indicate that the mutant strain gx217 showed a day delay in nodulation time and its abilities of nodule formation efficiency and competitive nodulation were apparently decreased compared with the parental strain gx201 respectively

    通過對野生型菌株gx201 ，突變體菌株gx217及其功能互補菌株rgx217進行植株試驗，發現突變體菌株gx217在結瘤時間方面較野生型菌株gx201慢一天，其結瘤效率及競爭結瘤能力均比gx201明顯降低。
  10. Secondly another vector phz1117 which for replacement of sc ( j11. 17 / scd78. 01, was constructed. phz1117 was transformed into wild type strain m145 and a gene replacement strain named hxy3 was obtained

    其次，構建了另一個用於置換scq11 . 17 scd78 . 01的置換載體phz1117 ，得到基因置換菌株hxy3並通過southern雜交驗證，與野生型m145相比，沒有觀察到hxy3有明顯的表型變化。
  11. The two end - points were localized on a 4. 3kb bamhl - ecor1 fragment of a cosmid 16c3 and a 1. 2kb sail fragment of a cosmid 17g7 respectively. the deletion junction was localized on a 2. 8kb bamhi fragment of the zx1 chromosome

    兩個端點分別定位在野生型菌株基因組文庫粘粒16c3的4 . 3kb的bamh1 - ecor1片段上和17g7的1 . 2kb的sal片段上，缺失界點定位在zx1染色體上的一個2 . 8kbbamh片段上。
  12. However, the content of coq10 in wild strains of psb is limited after all. through construction of recombinant strain producing coq10 and optimization of the culture medium and fermentation conditions of a selected strain, the content of coq10 in psb will be increased markedly. it can not only remarkably strengthen the effect of psb feed additive, but also lay foundation for the exploitation of coq10

    但野生型psb菌株coq _ （ 10 ）含量終究有限，如能選擇合適菌株進行培養優化，並通過構建基因工程菌，使菌體中coq _ （ 10 ）的含量成倍提高，則不僅可顯著增強psb飼料添加劑的應用效果，更重要的是，為coq _ （ 10 ）相關產品的研發奠定良好的基礎。
  13. ( 4 ) expressional plasmids containing the cloned pha synthase genes were constructed and transformed into wild type e. coli strains and e. coli fadb deleted mutant strain km32b

    （ 4 ）成功構建了含有上述型pha合酶基因的表達載體，並將它們分別轉入野生型大腸桿菌及脂肪酸降解fqdb缺夫突變株km32b中進行表達。
  14. The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively

    三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變， pcr產物與ppic3 . 5k連接，轉化大腸桿菌，獲得的混合質粒電轉化畢赤酵母gs115 ， omm平板篩選適于低溫或對熱穩定的重組子，篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ，其最活反應溫度為45 ，比野生型高出5 ； 40處理30min殘留活性為56 ，大大高於野生型的6 ；初始ph7 . 3528條件下培養72h ，發酵上清酶活為325u / ml 。
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