野芋實 的英文怎麼說
中文拼音 [yěyùshí]
野芋實
英文
common alocasia fruit-
To prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna. a recombinant prokaryotic expression vector, pet28 - mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully
本實驗另選用了原核表達質粒pet28 ( b ) ,根據已構建好的含有mbl野生型基因的t載體pgem - mbl ,設計一對引物, pcr擴增mbl基因,凝膠回收,雙酶切pcr產物和pet28 ( b )質粒, t4連接酶連接,轉化大腸桿菌dhsa ,氨芋選擇培養挑取克隆鑒定。
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