阿維黴素 的英文怎麼說
中文拼音 [awéiméisù]
阿維黴素
英文
avilamycin-
In addition to avermectins, s. avermitilis produces oligomycin, a strongly toxic compound. gene deletion vector pxl05 was used to disrupt oligomycin polyketide synthase ( pks ) encoding genes ( olma ) in streptomyces avermitilis cz8 - 73, the producer of anthelmintic avermectins b and the cell growth inhibitor oligomycin. olma gene cluster in the chromosome was displaced by deletion allele on the plasmid via double crossover
本研究以產阿維菌素b和寡黴素的阿維鏈黴菌cz8 - 73為出發菌株，構建了基因缺失載體pxl05 ，並將其轉入cz8 - 73中，通過缺失載體和染色體之間的同源雙交換，對染色體上長達90kb的寡黴素聚酮合酶（ pks ）基因簇（ olma ）進行了缺失。Avermectins are a series of 16 - membered macrocyclic lactones produced by streptomyces avermitilis with potent anthelmintic and insecticidal activity. they are the most effective agricultural pesticides and antiparasitic agents, and used widely in medical, veterinary and agricultural fields
阿維鏈黴菌（ streptomycesavermitilis ）產生的阿維菌素（ avermectins ）是一組高效、低毒、廣譜的殺蟲抗生素，在醫藥、農業和畜牧業生產中具有良好的應用價值和廣闊的市場前景。Shaking flask experiments and hplc analyses showed that the four mutants no longer produced the toxic oligomycin, and only made four components of avermectins b, which were avermectin b1a, b1b, b2a, b2b. the yields of avermectins b in these mutants were separately equal to those in cz8 - 73. this revealed that olma genes deletion did n ' t affect the biosynthesis of avermectins
將4株經southern雜交驗證正確的基因缺失突變株進行搖瓶發酵和hplc檢測，發現4個突變株均不再產生寡黴素而僅產阿維菌素b組分，阿維菌素的總產量和b1的產量與出發菌株相當，說明寡黴素pks基因簇的缺失並不影響阿維菌素的生物合成。In this study, we attempted to construct an engineering strain producing only avermectin bl through the replacement of dna encoding dh2 - kr2 domains of the avermectin pks ( avedh2 - kr2 ) with dna encoding dh2 - kr2 domains from the pikromycin pks in s. avermitilis olm73 - 12, producing only avermectins b and no oligomycin. gene replacement vector pxl201 ( pkc1139 : : 5 ' flank + pia : dh2 - kr2 + 3 ' flank ) was used to transform 5. avermitilis olm73 - 12 protoplasts
我們以不產寡黴素而僅產阿維菌素b的工程菌olm73 - 12為出發菌株，用委內瑞拉鏈黴菌（ streptomycesvenezuelae ）中編碼pikromycinpks模塊2上完全活性的dh和酮基還原酶（ kr ）的dna區域對olm73 - 12染色體上編碼阿維菌素pks模塊2中dh和kr的區域進行取代，試圖構建僅產b1組分的基因工程菌。Study on breeding of high - yield avilamycin - producing strains by nitrogen ion implantation
氮離子注入選育阿維拉黴素高產菌株的研究分享友人