陽明病證 的英文怎麼說

中文拼音 [yángmíngbìngzhèng]
陽明病證 英文
yangming syndrome
  • 陽 : Ⅰ名詞1 （太陽; 日光） the sun 2 （山的南面; 水的北面） south of a hill or north of a river 3 （中...
  • 明 : Ⅰ形容詞1 （明亮） bright; brilliant; light 2 （明白;清楚） clear; distinct 3 （公開;顯露在外;不隱...
  • 病 : Ⅰ名詞1 （疾病; 失去健康的狀態） illness; sickness; disease; malum; nosema; malady; morbus; vitium...
  • 證 : Ⅰ動詞（證明） prove; verify; demonstrate Ⅱ名詞1 （證據） evidence; proof; testimony; witness 2 （...
  • 陽明 : sunlight yang
  1. The high specificity of the dot - ppa - elisa was confirmed by specific blocking test and also by cross - reaction test. the diaphragm did not react with the antibodies against salmonellosis, streptococcosis, colibacillosis, chlamydiosis, hcv, ppv, prv, brucellosis. erysipelas, suis and chlamydiosis in cross - reaction test. the diaphragm has good sensitivity and could detect some pasteurella - positive test serum which has been diluted to2 - "

    試驗證明所建立的dot - ppa - elisa具有較好的特異性，與豬瘟、仔豬副傷寒、豬丹毒、豬細小病毒病、豬偽狂犬、豬布氏桿菌病、豬衣原體病陽性血清無交叉反應。
  2. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  3. The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences

    用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。
  4. At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

    在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
  5. The centre for health protection of the department of health today november 18 received a report from the university of hong kong that laboratory tests using reverse transcription polymerase chain reaction rt - pcr method on three out of the seven specimens taken from patients of the caritas medical centre outbreak yielded positive results to parainfluenza virus, but all the seven specimens were negative for other common respiratory pathogens

    生署生防護中心今日十一月十八日收到香港大學的報告，明愛醫院病童的七個樣本經大學完成反轉錄-聚合鏈反應rt - pcr測試后，其中三個樣本證實對副流感病毒呈陽性反應，但是所有七個樣本均對其他常見的呼吸道感染病原體呈陰性反應。
  6. The interesting gene fragment with ecori and noti were amplified by overlapping pcr, which inserted into vector plasmid ppic9k after degisted by ecori and noti, and the recombinant plasmid was transformed into competent dh5cc. positive clones were screened by pcr from the lb plate with amp. digesting analysis resulte shows that the interesting gene were inserted into the vector ppic9k with correct direction

    目的基因經雙酶切后連接載體ppic9k ，然後導入大腸桿菌dh5中，在含氨卞青霉素（ amp ）的lb板上用pcr反應篩選出陽性菌落，雙酶切結果表明目的基因已插入載體中，且方向正確，測序結果進一步證明人巨細胞病毒重組基因表達質粒成功地克隆了目的基因片段。
  7. Tests conducted by the public health laboratory centre under the centre for health protection ( chp ) of the department of health on nasal specimens collected from three students of caritas lok yan school today ( january 27 ) all yielded positive for rhinovirus, a common cold virus

    ?生署?生防護中心公共?生檢測中心抽取了三名明愛樂仁學校不適學童的鼻腔分泌樣本進行化驗，今日（一月二十七日）證實全部樣本對鼻病毒呈陽性反應，該病毒為一般傷風病毒。
  8. Tests conducted by the public health laboratory centre under the centre for health protection ( chp ) of the department of health on nasal specimens of the three fever cases reported yesterday ( january 30 ) in caritas medical centre ( cmc ) yielded positive for rhinovirus, a common cold virus, today ( january 31 )

    ?生署?生防護中心公共?生檢測中心抽取了於三名明愛醫院發燒院童的鼻腔分泌樣本進行化驗，今日（一月三十一日）證實全部樣本對鼻病毒呈陽性反應，該病毒為一般傷風病毒。
  9. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  10. As at 10 am today november 22, 30 of the 34 children affected in the outbreak of respiratory illness at the developmental disabilities unit of caritas medical centre were confirmed to be infected by parainfluenza type 4

    截至今日十一月二十二日上午十時，明愛醫院發展復康部出現集體呼吸道感染事件中受影響的34名病童中，有30人其樣本測試證實對副流感四型病毒呈陽性反應。
  11. As at 10 am today november 23, 32 of the 34 children affected in the outbreak of respiratory illness at the developmental disabilities unit of caritas medical centre were confirmed to be infected by parainfluenza type 4

    截至今日十一月二十三日上午十時，明愛醫院發展復康部出現集體呼吸道感染事件中受影響的34名病童中，有32人其樣本測試證實對副流感四型病毒呈陽性反應。
  12. As at 10 am today november 24, 32 of the 34 children affected in the outbreak of respiratory illness at the developmental disabilities unit of caritas medical centre were confirmed to be infected by parainfluenza type 4

    截至今日十一月二十四日上午十時，明愛醫院發展復康部出現集體呼吸道感染事件中受影響的34名病童中，有32人其樣本測試證實對副流感四型病毒呈陽性反應。
分享友人
分享到 Line

分享到臉書