隆化 的英文怎麼說
中文拼音 [lōnghuà]-
Cloning and sequencing of acc oxidase gene from sugarcane
氧化酶基因片段的克隆與序列分析Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。Using ethyl cyanoacetate, triethyl orthoformate and methylhydrazine as main raw materials, the herbicide pyrazosulfuron was prepared through the reactions of substitution, amination, cylclization, diazotization and chlorosulfonation
摘要以氰乙酸乙酯、原甲酸三乙酯和甲肼等為主要原料，通過取代、胺化、環化、重氮化和氯磺化等反應制得了除草劑吡嘧磺隆原料藥。Screening target ' gene of artemisinin antimalarials using drug - western from cdna expressing library : 12 b - deoxoartemisinyl - ( 4 ' - oxyacetic acid ) phenyl ether was linked to bsa by using of edc cross ! inker and the product acted as drug - probe
對重組pmd一18一t克隆載體及pqe一30表達載體雙酶切，提取tctp基因和pqe一30空載體並使二者重組，然後轉化m15 ，挑取陽Construction, expression and purification of his - hmgb1 and novel antigen in autoimmune hepatitis
1的克隆表達鑒定及蛋白純化Jiang bing on liaohe river culture park
盤錦興隆臺區委書記姜冰談遼河文化產業園Wheat cytochrome p450s catalyze metabolism chlorsulfuror
對綠黃隆的催化代謝作用Finally 4 right clones was obtained from 864 transformants by pcr detection. sequencing analysis showed that the hsa gene have been introduced into the right position of the human a - lactalbumin yac. furthermore, works to optimize the conditions of introducing the recombinant yac into goat f ibroblast via cell fusion was explorded
經pcr檢測，從864個轉化子中獲得了4個陽性克隆，測序表明，人血清白蛋白基因已正確的導入到人-乳白蛋白基因yac的特定位點上，並獲得了可進行細胞融合的重組yac載體。The meso - cenozoic evolution of bachu fault - uplift in tarim basin
塔里木盆地巴楚斷隆中新生代的構造演化Changeless and hopeless, the tumbrils roll along
不會變化，也沒有希望，死囚車隆隆地前進。In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli
根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。Gene cloning provides a means of purifying and propagating specific dna segments.
基因克隆化提供了一種純化和擴增特定DNA片段的方法。Cloning and sequence analysis of bos taurus hepatitis c virus core - binding protein 6 homologue gene
牛丙型肝炎病毒核心蛋白結合蛋白6同源基因的克隆化研究Cloning and sequencing analysis of sus scrofa hepatitis c virus core - binding protein 6 homologue gene
豬丙型肝炎病毒核心蛋白結合蛋白6同源基因的克隆化研究2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6. their antibodies " isotypes are tested by goad anti - mouse isotype regent
取馴化好並處于對數生長期的2 - e _ 4 - a和s _ 2 - b細胞，再常規融合和篩選，三次克隆化后得穩定分泌雙特異性抗體的雜交-雜交瘤細胞株6株。North term of river the renqiucity celebrate the xinlong chemical engineering the limited company to locate north china oil - field
任丘市鑫隆化工有限公司位於華北油田，主要從事高分子聚丙烯酰胺、高分子聚合物等系列產品的生產與銷售。The plasmids pci - mbl54 containing full length of mutations mbl cdna were propagated hi escherichia coli xl - 1 blue, then the extracted and purified pci - mbl54 were used to transfect dhfr ( - ) chinese - hamster ovary ( cho ) cells. after screeened with g418 and cloned, 4 g418 - resistent clones were randomly selected for detection of mrna expression by rt - pcr and molecular beacons. it was found that all of the 4 positive cell clones expresse mbl analogue as detected in transcription level
抽提、鑒定、純化重組質粒后，脂質體轉染法將重組質粒導入中國倉鼠卵巢細胞（ cho - dhfr ~ - ）中， g418選擇轉染子並克隆化培養，經rt - pcr和分子燈塔探針雜交鑒定其mrna轉錄，獲得4株穩定表達54位密碼突變型mbl的cho細胞。This dissertation is based on the regulation of arable land classification and degrading and the regular gis soft ware as well as the dbms technology, introducing the designing and development of the arable land classification information system on the base of the soft ware of mapinfo, which can be taken into three subsystems : the classification subsystem, the query and analysis subsystem and the dynamic updating subsystem
作者根據《農用地分等定級規程》和當前地理信息系統軟體平臺及通用的dbms技術，設計和開發了基於mapinfo軟體的隆化縣農用地分等信息系統。本文介紹了該系統的分等子系統、查詢分摺子系統和動態更新子系統基本原理和技術路線，並根據該系統在河北省隆化縣的應用對分等子系統進行了詳細介紹。Methods : the balb / c mouse is immunized with gene recombinant antigen p24 for four times in 2 months. the spleen cells of immunized mouse is hybridized with sp2 / 0 by peg, and the positive cell clones secreting the antibody to antigen p24 are detected by indirect elisa. through three clonings less diversed anti - p24 hybridoma cells are gained
方法：基因工程p24抗原免疫小鼠4次，歷時2個月，取脾細胞與骨髓瘤細胞株sp2 0 ，用peg融合， hat選擇培養和間接elisa篩選分泌抗p24抗體陽性的雜交瘤細胞，三次克隆化后得穩定分泌抗p24抗體的雜交瘤細胞株。The screening of supernatants was carried out with using indirect elisa. after subcloning by limiting dilution and selection. five clones of cells with high od value were acquired and named mvi
過陽性雜交瘤細胞的篩選和有限稀釋法的克隆化步驟，獲得了5株od值高的單克隆細胞株，編號為mv1 ， mv2 ， mv3 ， mv4 ， mv5 。分享友人