隆熱克 的英文怎麼說
中文拼音 [lōngrèkè]
隆熱克
英文
longeque-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后提取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片段,將此片段回收純化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。National is the world, song and dance troupe face the world, since it formed, has been went to russia, mongolia, tanzania, burundi, seychelles, japan, finland, sweden, american, france, poland, czech republic, germany, hungary, bulgaria, rumania, malaysia hong kong etc, has been welcome by the countries and areas, make great contribution to the friendship of all the world around, to propagate china and inner mongolia
蒲隆地、塞席爾、日本、芬蘭、瑞典、美國、法國、波蘭、捷克、德國、匈牙利、保加利亞、羅馬尼亞、馬來西亞、香港等國家和地區,受到所到國家和地區人民的熱烈歡迎,為增進中國人民與世界各國人民的友誼、為宣傳改革開放的中國和內蒙古做出了巨大的貢獻。It will provide us to further study the function of xanthophyll cycle in photoprotection. the major results are as following : two cdna sequences encoding violaxanthin de - epoxidase were cloned from japonica rice ( jrvde ) and indica rice ( irvde ) with the full - length of 1887bp and 1647bp, respectively. the homology of the open reading frame is 98 % identity between two rvde genes, and more than 60 % identities with those of other species
本論文從水稻和菠菜中克隆了編碼vde酶的基因,並通過轉基因植物進一步研究了葉黃素循環在熱耗散方面的作用,主要獲得了以下結果:首次從兩個水稻亞種(秈稻和粳稻)中克隆了rvde基因(分別命名為irvde和jrvde )的全長cdna序列,分別長1647bp和1887bp ,兩者開放閱讀框的同源性為98 ,與其它已知vde基因的同源性在60以上。2. an up - dated method was employed to purify tumv in this research. using the protease k method, we acquired the viral genome - rna. a pair of specific primers was designed and synthesized based on the nucleotide sequences of tumv coat protein genes reported before and rt - pcr was used to clone the cp genes of the six tumv isolates
應用改進的蕪菁花葉病毒的提取方法從病葉中提取病毒粒體,應用蛋白酶k法從病毒粒體中提取病毒rna基因組,根據已報道的tumv的cp基因序列,設計併合成了一對特異引物,利用rt - pcr法克隆了6個分離物的外殼蛋白基因,與克隆載體puc19連接后通過熱激法轉化大腸桿菌dh5 。3. the cold - heat method without the snailase and zymolyase was used as the pcr direct selecting of colony. to select the colony and suspend with 10 n 1 ddw, and then incubate
改進了重織酵母囪落的pcr鑒定方法:冷熱處理法,即挑取單克隆於10ul水中懇浮, m及5分鐘、液氮處理2分鐘, 9了c15分鐘,然後進行kr擴增。In our study we have cloned the osd gene from s. typhimurium by pcr, characterized the gene product and used this gene to construct asd + expression cloning vectors ptrc99a - asd
再將hpylori尿素酶b亞單位基因與尿素酶b和熱休克蛋白a融合基因分別克隆入ptrc99a一asd質粒的多克隆位點之內。And with the hot bats came some good old - fashioned excitement in the bronx
還有,一些像是來自舊時代布隆克斯區的火熱支援。Cloning and sequencing of heat shock protein 60 gene of helicobacter pylori
幽門螺桿菌熱休克蛋白60基因的克隆及序列分析Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis
將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 ( a ) +中,在t7啟動子下表達出融合蛋白,融合部分為硫氧還蛋白,位於類凝血酶基因上游,並在其n端帶6xhistag標簽以利於表達產物的分離純化,經熱激轉化至宿主菌bl21 ( de3 )中, iptg誘導斗小時后收獲菌體。Cloning and sequencing of heat shock protein 70 promoter of mycobacterium tuberculosis
結核分枝桿菌熱休克蛋白70啟動子的克隆和測序There are people who are obsessed in the experiments of cloning and wish to apply the theory of genetic reproduction in cloning human beings
某些熱衷於克隆試驗的人們想把遺傳復制的理論運用到復制人類上去。Expressions and significance of p 16ink4a and p14arf proteins in squamous carcinoma of the cervix
沙眼衣原體熱休克蛋白10基因的克隆及其在真核細胞中的表達In order to know whether the hau3r gene amplified by pcr had its natural activities, hau3r gene from phz2055 was cloned into phz1060, a streptomyces - e. coli bifunctional expression vector, to give phz2057. when e. coli dh5 a ( phz2057 ) was induced at 42c, large amounts of hau3r protein were produced to form inclusion bodies
將來自phz2055的hau3 ~ r基因克隆到大腸桿菌?鏈黴菌雙功能表達載體phz1060的啟動子p _ r下游的xba和ecor之間,獲得重組質粒phz2057 ,經熱誘導hau3 ~ r基因超量表達但仍以包含體形式存在。分享友人