雜交抗體 的英文怎麼說

中文拼音 [zájiāokàngtǐ]
雜交抗體 英文
hybrid antibody
  • 雜 : Ⅰ形容詞（多種多樣的; 混雜的） miscellaneous; varied; sundry; mixed Ⅱ動詞（混合在一起; 攙雜） mix; blend; mingle
  • 交 : Ⅰ動詞1 （把事物轉移給有關方面） hand over; give up; deliver 2 （到某一時辰或季節） reach （a cert...
  • 抗 : Ⅰ動詞1 （抵抗; 抵擋） resist; combat; fight 2 （拒絕; 抗拒） refuse; defy 3 （對等） contend with...
  • 體 : 體構詞成分。
  • 雜交 : [生物學] hybridize; cross; hybridization; cross breeding
  1. The experiment was conducted in a 2. 4 ha isolated field mimic rice production practice with pollen competition. a japonica gm rice l201 containing bar gene with herbicide basta resistance was used as a pollen donor and six indica hybrid rice varieties and its male sterile ( ms ) lines and two common wild rice ( oryza rufipogon and o. nivara ) that share same aa genome with cultivated rice were used as recipients

    本試驗選擇廣州作為華南水稻生態區的代表，以含bar基因（抗除草劑basta ）的轉基因粳稻l201為花粉供體，模擬大田生產實際，對轉基因向秈型兩系及三系雜交稻不育系、雜交稻品種及含aa基因組的普通野生稻的基因漂流及其影響因素進行了研究。
  2. By using these antibodies, which were raised to immunoreact with total proteins of purified mitochondria from different organs of mung bean seedlings, we find that there were two hybridizable aox bands in mitochondria in mung bean seedlings. their molecular weight was about 35kd and 38kd respectively

    將此抗體與來自綠豆幼苗不同器官線粒體的總蛋白分別進行western雜交，可觀察到綠豆幼苗線粒體中具有兩條清晰的aox雜交帶，它們的分子量分別為35kd和38kd 。
  3. 2 - e4 and s2 is induced respectively by 8 - ag and 5 - brdu with different drug concentration to make them deficient in hypoxanthine - guanine phosphoribosyl transferase ( hgprt ) and in thymidine kinase ( tk ) respectively and renamed 2 - e4 - a and 82 - 6. their antibodies " isotypes are tested by goad anti - mouse isotype regent

    取馴化好並處于對數生長期的2 - e _ 4 - a和s _ 2 - b細胞，再常規融合和篩選，三次克隆化后得穩定分泌雙特異性抗體的雜交-雜交瘤細胞株6株。
  4. However, it is necessary to acquire the antibody or the antiserum, which could specially react with the expression protein of die objective gene transferred into the transgenic plant according to the characteristics of high homology and immune cross - reaction among plant ferritin, using the special immune serum of pea ferritin, the content of plant ferritin could be detected for studing the ferritin expression of transgenic plant by the technique of immunoassay such as immunoprecipitation, eljsa and western blotting

    利用免疫檢測技術進行植物轉基因的表達檢測是一種簡單、靈敏、快速、可靠的方法，但其前提條件是要有與轉基因植物目的基因表達的蛋白質發生特異性免疫反應的抗體或抗血清。根據植物鐵蛋白之間有高度同源性和交叉免疫反應的特性，利用特異性的豌豆鐵蛋白抗血清，就可通過免疫沉澱、 elisa或western雜交等免疫檢測方法進行植物鐵蛋白含量等的檢測，從而更好地進行轉基因方面的研究。
  5. The ascites fliud titer of the other hybridoma s2 for further hybridization is 1 : 16000 by blood agglutination. the isotype of 2 - e4 is igg2a, and s2 is iggs

    用於再雜交的另一細胞株為本室保存的分泌抗人a型紅細胞單克隆抗體的雜交瘤細胞株s ； ，其血凝效價為l ： 16000 。
  6. Guan xiaohong ( 1991 ) established a cell line of the monoclonal anti - idiotypic antibody ( anti - id ) np30 of schistosoma - 6 - japonicum, whose isotype was identified to be igm and which was testified to be internal image of gaa

    分別以日本血吸蟲單克隆抗獨特型抗體wbo的雜交瘤細胞株總基因組dna和其提取rna進行rticr合成的cdna第一鏈為模板，擴增v 。 、 v 。
  7. Preparation and characterization of monoclonal antibody against botulinum neurotoxin type b

    型肉毒毒素單克隆抗體雜交瘤株的建立
  8. Frankfurt os, robb ja, sugar baker ev, et al. monoclonal antibody tosingle2stranded dna is specific and sensitive cellular marker of apoptosis [ j. exp cell res, 1996, 226 ( 2 ) : 3872397

    吳國慶，李少華，徐志偉，等.抗單鏈單克隆抗體雜交瘤細胞株的建立及鑒定[ j ] .細胞與分子免疫學雜志， 2002 ， 18 （ 5 ） : 4792480
  9. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  10. 2 - e4 - a and 82 - 6 are hybridized during their log growing time, and the hybrid - hybridomas are cloned for 3 times and produce 6 hybrid - hybridoma cells. the chromatosome of hybrid - hybridoma 3 - hu and hybridoma 2 - e4 - a and s2 - b are counted, and the antibody of ascites fluid or culture supernatant of 3 - hn is prepared. the positive clones are detected by three methods at the same time : rbc agglutination for monospecific anti - human rbc type a antibody, indirect elisa for anti - p24 antibody, and rbc solid - phase adherence for bispecific antibody

    選其中一株3 - h _ （ 11 ）做雜交-雜交瘤細胞染色體計數，同時計數兩母株2 - e _ 4 - a和s _ 2 - b的染色體數：制備腹水型和上清型抗體，用三種方法同時檢測其中的雙特異性抗體、單特異性抗人紅細胞抗體和抗p24抗體，即紅細胞固相吸附法測雙特異中文摘要性抗體，紅細胞凝集試驗測單特異性抗人a型紅細胞抗體，間接elisa法測抗p24抗體；用腹水型抗體做耐熱性及耐凍融實驗。
  11. Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays

    為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。
  12. The expressed product was lysised with supersonic wave and purified by sds - page. elisa analysis revealed that the antigenicity of the vpi protein has been detected. the forth part - detection of aev - nh937 strain by in situ hybridization ( 1sh ) - probe was labelled with digoxigenin ( dig ). then the probe hybrid with 5d, 10d, and 20d postinfection brain tissue of chicken. the results of the ish showed that the positive signal was found in 3 cases, while control group was negative. there has been a reasonable correlatien between this method and other detection test

    經超聲波裂解，用尿素溶解包涵體，電泳純化后，利用elisa檢測vp _ 1外殼蛋白，表明具有一定抗原性；第四部分：應用原位雜交檢測aevi用dig標記的探針與sd 、 10d 、 20d的攻毒雞腦組織雜交，來檢測那v的rna 。
  13. Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )

    利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。
  14. It has been demonstrated directly or indirectly - 7 - that ak auto ab is an important element in the immune network and plays a important role in maintaining physiological functions, clearing aged cells and metabolic products, regulating immune responses and protecting against infection. in some pathological states such as psoriasis and contact dermatitis, a certain serum level of the antibody could inhibit the progression of the diseases, and is beneficial to the recovery from the diseases. after a long time studies on the production and regulation mechanism of physiological and pathological auto antibodies, meanwhile, experiencing an intensive academic debating on whether naas a " horror autotoxicus " or a " gnothi seaution ( know yourself ) ", a common viewpoint has been achieved that naa is of clinical significance in the treatment of immunity diseases for it ' s function in the immune system stability, immunoglobulin y and polyclonal ak auto abs have been used in treating inflammatory dermatitis, and recombinant antibody is under investigating

    抗角蛋白自身抗體（ akautoab ）是naa的重要組成部分，以往實驗通過雜交瘤技術、免疫親和層析技術和噬菌體抗體庫技術分別獲得單克隆akautoab 、健康人血清多克隆akautoab和基因工程人akautoab ，並對akautoab免疫學特性及在體生理和病理意義進行了廣泛的研究，直接或間接地發現akautoab是機體正常免疫調節網路的組成部分，在維護某些生理狀態的穩定、清理衰老細胞及代謝產物、調節免疫和抗感染等方面起到重要作用；在某些病理情況下（如銀屑病、接觸性皮炎等） ，體內akautoab的組分和滴度會發生變化，而正常水平的akautoab則有利於限制病情的發展，促進損傷的修復。
  15. Pcr, pcr - southern blot analysis, southern dot blot analysis of lettuce dna confirmed that adw gene had been integrated into the plant genome. the results also showed that the transformation frequency of pb - adw was higher than that of pbg - adw, which suggested that camv35s promoter would be better than pi ii promoter in the case of transgenic lettuce

    （ 3 ）細胞核載體pb - adw 、 pbg - adw均採用農桿菌介導法將adw導入萵苣，細胞核轉化獲得了生長良好的抗性萵苣植株，經pcr 、 pcr - southern 、 southern斑點雜交分析證實， adw基因已整合到萵苣基因組中。
  16. Results : gain 5 hyhridoma cells secreting anti - p24 antibodies, in which 2 - e4 is selected for further hybridization and its ascites fliud antibody titer is 1 : 1600000

    細胞株作為再雜交的備用細胞，其腹水型抗體的elisa效價為1 ： 160萬。
  17. Sds - page showed efficient expression and secretion of the rhfl. the highest yield ( 108 mg / l ) was obtained when expression was induced with 0. 5 % methanol for 96 hours

    Western雜交實驗出現了可被抗體特異性識別的雜交帶，證實此條帶即為重組畢赤氏酵母km7lppicgkil分泌表達的重組fl蛋白。
  18. In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer

    應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。
  19. 1975 hybridoma technology deeloped for production of monoclonal antibodies

    1975年，雜交瘤技術被應用於生產單克隆抗體
  20. 1975 hybridoma technology developed for production of monoclonal antibodies

    1975年，雜交瘤技術被應用於生產單克隆抗體
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