雜交探針 的英文怎麼說

中文拼音 [zájiāotànzhēn]
雜交探針 英文
hybridization probe
  • 雜 : Ⅰ形容詞（多種多樣的; 混雜的） miscellaneous; varied; sundry; mixed Ⅱ動詞（混合在一起; 攙雜） mix; blend; mingle
  • 交 : Ⅰ動詞1 （把事物轉移給有關方面） hand over; give up; deliver 2 （到某一時辰或季節） reach （a cert...
  • 探 : Ⅰ動詞1 （試圖發現） try to find out; explore; sound 2 （看望） call on; visit; see 3 （向前伸出）...
  • 針 : Ⅰ名詞1 （縫衣物用的工具） needle 2 （細長像針的東西） needle like things 3 （針劑） injection; sh...
  • 雜交 : [生物學] hybridize; cross; hybridization; cross breeding
  • 探針 : probe; sound; filling fork; feeler; explorer; probing pin; touch needle; wire probe
  1. Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china. cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively. total rna extracted from field samples were used for rna dot - hybridization

    用侵染馬蹄蓮的dsmv3末端序列和黃瓜花葉病毒（ cmv ）的亞基因組啟動子區互補dna序列為標記探針，對自然感病的天南星科植物進行rna斑點雜交，並結合雙鏈rna分析、病毒提純和形態學觀察，對杭州等地16屬天南星科植物的81個樣品進行了病毒鑒定。
  2. Gus examinaton showed that the rate of transgene was higher. and pcr analysis, pcr - southem and southern analysis all showed that chs was intigrated into quamocliy pennata chromosome successfully

    同時，還以dig標記的camv35s上的一段為探針進行southern點雜交和pcr - southern雜交。
  3. To dress the question if other virulence gene were present in this kind of strains, 152 of 436 irp2 - hybridized strains were re - confirmed and selected for this study. the virulence genes or putative virulence genes detected by pcr or hybridization include heat stable toxin ( st ) & heat labile toxin ( lt ) for enterotoxigenic e. coli ( etec ), invasive plasmid antigen b ( ipab ) for enteroinvasive e. coli ( eiec ), epec adherence factor ( eaf ), epec secretion protein c ( espc ) for enteropathogenic e. coli ( epec ), hemolysin ( hlya ) and shiga toxins ( sltl and slt2 ) for enterohaemorrhagic e. coli ( ehec ) and eaggec probe for entero - aggregative e. coli ( eaggec ). the prra and yc73 genes of pathogenicity associated island ( pai ) of urepathogenic e. coli ( upec ) and " o " island 28 ( rtx 615 ) gene was also detected, the later was a newly discovered putative pathogenicity island in e. coli o157 : h7

    為探討攜帶小腸結腸炎耶爾森氏菌的hpi毒力島的大腸桿菌是否具有其他已知的毒力基因，選取82株由原位雜交和pcr方法初篩irp2陽性的大腸桿菌菌株，進行在致瀉性大腸桿菌的25個毒力基因的檢測，包括腸產毒性大腸桿菌的熱穩定毒素st和熱不穩定毒素lt ，腸侵襲性大腸桿菌的侵襲蛋白b基因ipab ，腸致病性大腸桿菌的eaf 、 espc基因，腸出血性大腸桿菌的溶血素hly 、志賀毒素1 （ slt1 ） 、志賀毒素2 （ slt2 ）基因，腸集聚性大腸桿菌的eaggec探針，以及在泌尿道致病性大腸桿菌和o157 ： h7大腸桿菌中新發現的毒力島基因。
  4. Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city

    最後，用地高辛隨機引物法標記成momp基因核酸探針，斑點雜交檢測衣原體基因組dna ，靈敏度可達10pg ，且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較，初步證明該探針具有較高的敏感性與較強的特異性。
  5. Fingerprints of 5 strains of the inbred mice and 2 strains of the inbred rats was conducted using a nonisotopically hrp labeled jl - 02 by the second institute of the public safety bureau of china and southern blot hybridization, the author studied many fingerprints of the same dna, the different organic fingerprints of the same organism and fingerprints of parent and offspring. the patterns were completely different among the different strains and those of the samples from the same strain were completely identical

    採用公安部二所自行研製的jl - 02多位點探針對5個品系的近交系小鼠和2個品系近交系大鼠進行了dna指紋分析，經過對同一dna的反復製作dna指紋圖和同一個體不同組織進行的dna指紋圖製作及對親代和子代（同品系內和不同品系間雜交）間的dna指紋圖比較。
  6. Four c - ch3, six aromatic carbons, six carbons of mycosamine were the characteristics of candicidin d, one ketal corresponding to mycosamine and four ketons indicated that no hemiketal formed between c - 15 and c - 19 in fr - g08b or candicidin d. however, such hemiketal was usually thought having been formed

    用這個探針，對鏈黴菌fr - 008的基因文庫進行雜交篩選，獲得了3個陽性克隆，經southern雜交分析，發現它們均含有6 . 4kb共同的陽性片斷。
  7. So streptomyces sp. fr - 008 is a new strain synthesizing candicidin. nmr studies analysis of the 1h - 1h cosy spectrum allowed us to distinguish some important chemical shifts of the protons in fr - 008b which could be identified as eandicidin d. especially for amino - mycosamine residue, methyl protons and all the protons on the ring could be defined by correlating relation

    利用與糖基合成有關的基因str - de作探針對這兩個菌株的總dna進行了southern雜交，從兩個菌株的總dna中均檢測到一段相同大小的陽性片斷，約6 . 4kd （ bamhi + bglii酶切） 。
  8. Green fluorescent protein ( gfp ) gene was conjugated to the 3 " end of the pap gene in order to screen easily of the transgenic cotton plants. the combined gene was cloned into plant expression vector pbi121 and then transformed. about 5000 seeds of the transgenic cotton were obtained and the some seedlings of the transgenic cotton could give a bright green fluorescence in the dark condition when the cotton seedlings were irradiated with ultraviolet rays

    為了便於轉基因棉花後代的篩選，在pap基因的3 』端融入了綠色熒光蛋白gfp ）基因，然後將融合基因克隆在植物表達載體pbi121上，再進行遺傳轉化，得轉基因棉花種子5000餘粒，將種子播種長到于葉展開時，先在黑暗中用紫外燈照射，查找表現綠色熒光的幼苗，然後再用地高辛（ dig ）標記的pap基因特異性探針對這些棉花進行點雜交，最後發現有8株棉花表現陽性反應，說明pap基因的確己經轉到了棉花的基因組中，其棉花黃萎病的抗性鑒定正在進行之中。
  9. The expressed product was lysised with supersonic wave and purified by sds - page. elisa analysis revealed that the antigenicity of the vpi protein has been detected. the forth part - detection of aev - nh937 strain by in situ hybridization ( 1sh ) - probe was labelled with digoxigenin ( dig ). then the probe hybrid with 5d, 10d, and 20d postinfection brain tissue of chicken. the results of the ish showed that the positive signal was found in 3 cases, while control group was negative. there has been a reasonable correlatien between this method and other detection test

    經超聲波裂解，用尿素溶解包涵體，電泳純化后，利用elisa檢測vp _ 1外殼蛋白，表明具有一定抗原性；第四部分：應用原位雜交檢測aevi用dig標記的探針與sd 、 10d 、 20d的攻毒雞腦組織雜交，來檢測那v的rna 。
  10. One was cloning, sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ), the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot

    為了鑒別診斷tgev與prcv及對tgev進行流行病學調查，本研究採用原核表達系統（ gst融合表達系統）表達tgev纖突蛋白（ s蛋白）中含有b和c抗原位點的多肽，並且制備了非放射性地高辛標記的核酸探針，通過斑點雜交（ dot - blot ）檢測tgev核酸rna 。
  11. The divergent sequences of rdna from s. costatum are used to design primers meeting the requirements of the rfq - pcr. seven pairs of primers are designed and designated as primer 1 ( f / r ) ~ primer 7 ( f / r ), respectively, among which primer 1 ( f / r ), primer 2 ( f / r ), primer 5 ( f / r ), primer 6 ( f / r ) showed high level of specificities to s. costatum. then, the pcr products primed by primer6 ( f / r ) are sequenced

    首先以中肋骨條藻的rdna序列為設計種特異性引物的靶區域，共設計出7對適合rfq - pcr的引物（依次命名為primer1 （ f r ） primer7 （ f r ） ） ，並用常規pcr實驗初步證明其中有4對引物（ primer1 （ f r ） 、 primer2 （ f r ） 、 primer5 （ f r ） 、 primer6 （ f r ） ）可作為中肋骨條藻特異性引物的候選者；然後測定了以primer6 （ f r ）為引物的pcr產物的序列，序列分析表明，中肋骨條藻的pcr產物序列與其他藻的pcr產物序列差別較大，從中可設計出滿足rfq - pcr需要的taqman探針（命名為taqman6 ） ；進一步的核酸雜交實驗表明， taqman6隻與中肋骨條藻的pcr產物雜交，不與其他藻的pcr產物雜交。
  12. Capacity evaluation of different media for isolating predominant phenol - degrading bacteria from activated sludge with community structure - specific dna probes

    微生物群落結構探針雜交評價不同培養基從活性污泥分離優勢菌群的能力
  13. The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences

    用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。
  14. Due to these inherent advantages, ecl method has attracted much attention from all analytical fields, especially from biochemical analysis. in this dissertation we focused on the preparation of a new type of dna probes which were labeled with ecl activated substances. based on coupling with the dna hybridization and immobilization techniques, we have developed new ecl methods for the determination of special dna sequence

    本論文通過研究了多種ecl活性物質的發光性能，並以這些物質為標記物制備了多種高靈敏度的dna - ecl探針，結合dna雜交技術和dna固定化技術，將高靈敏度的ecl檢測手段應用於生命物質dna的序列識別及含量測定，為dna傳感器的研究和基因晶元的開發提供了新的思路和方法。
  15. 3. using scanning tunnel microscopy ( stm ) to observe microcosmic change between biomolecule and gold particle on the surface of lsaw biosensor during the process of probe immobilization and hybridization, also the naked gold membrane

    3 .利用掃描隧道顯微鏡觀察傳感器裸金膜表面、探針固定、核酸雜交過程中生物分子與金顆粒之間的微觀變化。
  16. 6, we used gcn5 and rpd3 genes as probes to detect the homologous sequences in drosophila melanogaster by fluorescence in situ hybridization ( fish ). this work has provided useful information for the localization and cloning of related histone acetyltransferase and histone deacetylase genes in drosophila melanogaster

    6 ，利用己獲得的酵母gcns和rpd3基因為探針，對果蠅多線染色體進行原位雜交實驗，試圖找出與gcns和rpd3基因同源的基因片段。為今後克隆和分離果蠅中與乙酞化和去乙酚化相關的基因奠定基礎。
  17. System for detecting single nucleotide polymorphisms based on hybridization probes

    熒光雜交探針技術檢測單核苷酸多態性
  18. Another detection method, dot blotting, was built also based on 35s promoter and nos terminator. the two genes were labeled by digoxin to done as probe used in dot blotting

    並將35s啟動子和nos終止子用地高辛標記后制備成基因探針，建立了轉基因檢測的分子雜交技術?點雜交。
  19. Differential screening for the library revealed that, 167 of 237 clones randomly selected from the library are positive and represent genes that were exclusively or intensively expressed in bract, which contain inserts ranging from 100 to 2000 bp in length. moreover, except a few clones were expressed predominantly in bract, absolute majority of subtraclive clones were expressed at low level in all checked organs, i. e., bract, leaf and root

    用地高辛標記的珙桐苞片、葉片和根的cdna做探針，與差減雜交所獲得克隆的pcr產物的雜交結果表明，有少數基因在這些器官中都是大量表達，而大多數在這些器官里的表達量均很少，除苞片外，一般均檢測不到，有部分基因在苞片中優勢表達。
  20. A pair of primer, dig labeled probe and a taqman probe based on the conserved nucleotide sequence of cp gene of different pnrsv strains were designed and synthesized

    本文根據病毒各株系外殼蛋白基因的保守序列，設計了雜交誘捕探針、 dig標記雜交探針以及確定了最佳誘捕參數和雜交檢測參數，建立雜交誘捕rt - pcr ? elisa 。
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