雞胚接種 的英文怎麼說
中文拼音 [jīpēijiēzhǒng]
雞胚接種
英文
egg inoculation-
Two ndv strains, lasota and f48e9, were propagated in 9 - day - old embryonated specific - pathogen - free ( spf ) eggs respectively for 36 hours at 37, then these chicken embryos were placed at 4 for 12h before the allantoic fluid was gained
首先，將ndvlasota株和f48e9株分別接種於9日齡spf雞胚，於37孵化36h ，置4 12h后收獲雞胚尿囊液。The chickens and chicken embryos were inoculated with variant serotype isolate e of infectious bursal disease virus using cloacal, nosal routes or via the allantoic cavity route, and the histopathological features of the bursa of fabricius of the ibdv _ infected chickens at various intervals of time were systematically investigated
本試驗全面而系統地觀察了傳染性法氏囊病病毒變異e株，通過泄殖腔、鼻腔和尿囊腔接種雛雞和雞胚后不同時間法氏囊的組織形態學變化。Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks
生物學特性：雞胚尿囊液經離心、磷鎢酸負染后，電鏡觀察該病毒為典型的冠狀病毒；該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用，當繼代到第5代后，胚體嚴重病變；病毒在雞胚中隨著接種時間的延長，其效價增高， 96h可達到48h的2倍；該毒株可在cef上生長，但不能形成明顯的蝕斑；經1胰酶處理后可凝集雞紅細胞；雞胚的第四代尿囊液病毒回歸動物體，病死雞腎臟呈典型的花斑腎，腺胃則未見肉眼可見的病變。The virus was purified by ultracentrifuge. according to the ns gene sequence published by genbank, one primer t - 1 was designed to amplify the cdna by reverse transcript. the other primers nsl - u / ns1 - 1 and ns2 - u / ns2 - 1. hns2 - u were designed to amplify the ns1, ns2 and hns2 gene
本實驗用接種雞胚的病毒增殖方法獲得了a / chicken / mudanjiang / 0823 / 00 （ h9n2 ）分離株的大量增殖，增殖病毒經差速離心進行純化，經超迷離心濃縮。The research consist of four parts. the first part is multiplication, purification and electron microscope examination of the avian encephalomyelitis virus. a 1 : 5 dilution of isolate - nh937 of aev and control group of pbs were inoculated to susceptible 6 - day - old chickens embryos. respectively. after incubation for 10 days, the urinay vesicle liquid was collected. making a comparison the size of the chickens embryos between the test group and the control group, the results showed that the size of the control group is bigger than that of the test group. purified virions were examined under the electron microscope, the result revealed that there are a lot of virions and the aev - nh937 was multiplicated in embryos. the second part was seguence analysis of the genome of the aev - nh937. nine pairs of primers were designed according to published calnek vaccine strain of aev
本研究共分四個部分：第一部分為aev的增殖，純化和電鏡觀察，用1 : 5倍稀釋的aev - nh937株和陰性對照pbs分別經卵黃囊接種於6dspf雞胚，繼續孵化10d后，收集尿囊液。比較接種組和健康對照組雞胚的大小，結果顯示，健康對照組雞胚明顯大於接種組。分離、提純aev ，把純化的病毒在電鏡下觀察，證明確有大量aev病毒粒子存在，說明aev在雞胚中成功擴增；第二部分是aev - nh937基因組的序列測定工作。Materials and methods : fecal, cloacal and tracheal swabs from different types of poultry were collected in 6 live - bird retail markets once a week. they were inoculated into 9 - 11 days embryonated chicken eggs and incubated in 35 " c for 72 hours. hi and nl assays were performed to detect the subtype of viruses if ha test were positive ( ad ^ 16 ) and contamination test were negative
材料和方法：每星期收集一次標本，收集的標本常規處理， 9 - 11日齡雞胚尿囊腔接種， 35培養72小時，收取尿囊液，血凝陽性（ ad 16 ）且細菌培養陰性者- 70保存並進一步做hi ， ni實驗鑒定亞型。The information generated in current study suggests that the developing influenza ecosystem in southern china region may play an important role in the process of emerging novel influenza viruses, even directly impact the genesis of pandemic influenza strains. materials and methods : fecal, cloacal and tracheal swabs from different types of poultry were collected in live - bird retail markets once a week. they were inoculated into 9 - 11 days embryonated chicken eggs and incubated in 35 for 72 hours
本課題意在: 1 、以pa和cu為代表，探討hgnz亞型流感病毒在這些新形成的小種群中的流行情況; 2 、探討這些小種群中流感病毒的來源:是從其它動物跨種屬傳遞而來，還是本身為流感病毒的天然宿主: 3 、探討這些新形成種群中hgnz亞型流感病毒的進化情況，以及其在整個流感病毒生態體系中的作用;材料和方法:每周採集標本一次，常規處理后，接種於9一n日齡雞胚尿囊腔， 35恆溫培養72小時，收取雞胚尿囊液。12 - day - old spf chicken embryo was inoculated with various concentration of aiv ( h5 subtype ). then allantoidal fluid was collected and virus titer was measured
將禽流感病毒（ h5n1 ）接種12日齡spf雞胚，所得尿囊液濃縮后，一步法提取病毒rna 。And cut open the symptom of examining and infer from the ill chicken ' s only appearance for mycoplasma gallisepticum infection, through isolating germs from the disease chicken, the separation that and then carry on the bacterium trains and check with the microscope, the inoculation agglutinate experiment of the whole blood of chicken ' s embryo, confnm as the mycoplasma gallisepticum infection at last
從患病雞只的外觀和剖檢癥狀推斷為支原體疾病，通過從病雞中分離病原微生物，進而進行分離培養和顯微鏡檢查，雞胚的接種、全血凝集實驗，最後確認為禽的支原體疾病。分享友人