離子電泳 的英文怎麼說
中文拼音 [lízidiànyǒng]
離子電泳
英文
ionophoresis-
This system contains : parts load, pre - degrease, degrease, spray cleaning, spray surface adjustment, spraying phosphating, rinse with d - ionized water, electro - deposition, ultra - filter for post rinse, oven curing, cooling and unload
由上件,預脫脂,脫脂,噴洗,噴淋表面調解,噴淋磷化,去離子水噴洗,電泳塗裝,超濾液噴洗,漆膜固化,自然冷卻,下件工序組成。Electrophoresis is a technique that separates macromolecules according to their net electrical charge and shape.
電泳是根據分子的靜電荷和形狀分離大分子的技術。To prove up ulteriorly the components of ants, the author has separated and purified the polypeptides of formica rufa linnaeus and studied their biologic and officinal activities. at the same time, the author has mensurated the molecular weight of polypeptides by sds polyacrylamide electrophoresis to analyze the polypeptides quantific - ationally. in order to provide the scientific basis for studying and empoldering the ants, we have done these studies
為進一步探明螞蟻體內的活性成分以開發和利用其藥用價值,本文對紅褐林蟻的提取物中的多肽進行了分離、純化並對其生物活性和藥用活性進行了研究,通過電泳測定了多肽的分子量,從而為進一步研究開發螞蟻提供科學的依據。6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page
將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析, blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟,得到聚丙烯酰胺凝膠電泳為單一蛋白區帶,比活為1 . 46u mg的酶制劑。二 、 effects of calcium concentration on the membrane - bound gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation. the membrane - bound gq a was extracted from the retina of the macrobrachium rosenbergi with protein extract and was electophoresised by sds - page. the percent of the membrane - bound gq a was analyzed by tanon gis gel image disposal system
二、鈣離子濃度對光暗適應羅氏沼蝦感光細胞膜結合gq蛋白亞基的影響用蛋白質提取液提取膜結合gq蛋白亞基並sds - page電泳,利用tanongis凝膠圖像處理系統分析其含量。In our experiment, after light and dark adaptation, the retina of the macrobrachium rosenbergi was respective incubated in high calcium solution, physiological solution and low calcium solution. we studied the effect of calcium concentration on the content and subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation by sds - page technology and imunoelectron microscopy technology. our study results indicated : 一 、 effects of calcium concentration on the soluble gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation
而鈣離子對gq蛋白亞基活性有無影響還未見報道。我們以光適應和暗適應條件下的羅氏沼蝦復眼視網膜為材料,分別用高鈣溶液、生理溶液、低鈣溶液孵育后,通過sds ? page電泳技術及免疫膠體金電鏡技術,研究鈣離子濃度對光暗適應時羅氏沼蝦感光細胞gq蛋白亞基含量的影響及亞基亞細胞定位的影響。In the part, the author ' s intention is mensurating the molecular weight of the polypeptides by sodium dodecyl sulfate polyacrylamide gel electro - phoresis. the result shows that one of the polypeptides which we have mensurated is about 17, 000da. the conclusion is that the components which we have separated and purified are small molecule polypeptides. 5
紅褐林蟻多肽蛋白質的分子量測定本部分旨在通過sds -聚丙烯酰胺凝膠電泳測出所得多肽蛋白質的相對分子量,測得其中一組分的相對分子量為17000da左右,結論為所分離、純化的物質是小分子的多肽。In order to produce monoclonal antibodies, first, several v. dahliae isolates were grown in liquid czapeak medium, after rinsing mycelia and eliminating zoospores, the fungal tissue was homogenized with the pestle in liquid nitrogen and then transferred to test tubes and was centrifuged in tris - hcl buffer
在制備抗原的過程中,首先液體振蕩培養了若干株棉花黃萎病菌,經過沖洗除孢子、液氮研磨,用tris - hcl抽提,再離心制得菌絲蛋白提取液,可作為電泳樣品。Using diethanolamine as aminating agent and glacial acetic acid as neutralizing agent, aminated epoxy acrylic cationic resin was prepared. the effect of technology of aminated epoxy acrylic resin on properties of eletrodeposition was studied by conductivity meter and electrophoresis apparatus. it was shown that, conductivity firstly decreased, and then increased with aminating temperature increase. in contrast with putting polyacrylic resin into thin acetic acid solution, the more compact film could be achieved by neutralizing polyacylic resin with glacial acetic acid and then add it into water. when neutralizing temperature was enhanced, the speed of electrodepsidon was found to increase, and the film was also more compact. increasing the dn leads to enhanced conductivity and smaller particle size. when dn equaled to 80, the smoothest film could be achieved
以二乙醇胺為胺化劑、冰醋酸為中和劑,合成了胺化環氧丙烯酸陽離子樹脂.採用電泳儀和電導率儀,研究了胺化環氧丙烯酸樹脂合成工藝對陰極電泳塗料電沉積性的影響.結果表明,隨著胺化溫度的增加,電泳液電導率先下降後上升.將冰醋酸加入樹脂中中和,後用水稀釋,比樹脂在醋酸稀溶液中中和,電沉積性能更好.電沉積速率隨著中和溫度的上升而增加,電沉積膜緻密性相應增加.中和度( dn )愈高,電泳液電導率愈大,粒徑越小,而塗膜外觀在中和度為80時達到最佳Cataphoretic migration speed
陽離子電泳遷移速度Medium experiments were arranged under uniform design, and then an optimum medium was got accordingly. the culture liquid was centrifugalized at 3, 500r / min for 30min, then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others
通過硫酸銨分級沉澱、 deaesephadexa - 50陰離子交換凝膠層析和sephadexg - 75凝膠柱層析對發酵液進行分離和純化,並得到電泳純的酶。All of these indicated that arsenic trioxide was a powerful chemotherapeutic agent and the cells in the treatment group were induced to apoptosis. to further understand the molecular mechanisms of arsenic trioxide treatment in the induced cellular apoptosis, we applied the restriction display - pcr ( rd - pcr ) technique combined with polyacrylamide gel electrophoresis and sliver staining techniques to separate the differentially expressed genes
為了進一步闡述as _ 2o _ 3作用的分子生物學機制,我們首先應用rd - pcr技術,結合聚丙烯酰胺凝膠電泳和銀染技術分離和顯示了as _ 2o _ 3作用k562細胞前後的cdna片段,發現了11個差異表達的基因片段。By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles
對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現,抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應;進而通過免疫金標結合電子顯微鏡觀察發現,大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上;統計結果表明,類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution
3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵,用緩沖液抽提后,經硫酸按沉澱, deae一纖維素離子交換層析,聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶,用sds一page檢測為一條均一譜帶,其分子量約為78kd 。- acetolactate decarboxylase are widely found among bacterial strains but not in other groups of organisms. the enzyme has been demonstrated to be effective for removal of acetolactate and widely used in beer product. in this paper, - acetolactate decarboxylase from bacillus subtilis was purifed to homogeneity from cell extract by ammonium sulfate - fractionation, heat treatment, deae - sepharose fast flow column chromatography
本文對來源於枯草芽孢桿菌( bacillussubtilis ) 3226 - 5的-乙酰乳酸脫羧酶經硫酸銨分級沉澱、熱處理、 deae - sepharosefastflow離子交換柱層析等分離純化步驟,得到sds - paeg電泳純,通過n末端氨基酸序列分析驗證酶蛋白的純度。A mixture of three amino acids ( arg, gly, glu ) labeled with fluorescein isothiocyanate ( fitc ) was separated in pdms microfluidic chip, the separation voltage is 200v / cm, the separation time is less than 120 seconds ; according to ccd fluorescence images, two distinct physical processes - stacking and destacking during sample injection were studied qualitatively ; rhodamine b, a kind of temperature - dependent fluorescence dye, was used as probe to develop a temperature - fluorescence intensity equation, then temperature - color map in microchannels was constructed, and temperature trait in microchannels on the pdms microfluidic chip was analysed. according to the results, we conclude that the electric field applied to the pdms microfluidic chip should not exceed 400v / cm
利用pdms微流控晶元對fitc標記的精氨酸、甘氨酸、谷氨酸混合物進行了電泳分離,分離電壓為200v cm ,分離時間不到120秒;通過拍到的熒光顯微圖像對電泳注樣過程中復雜的樣品分子積聚與解聚現象作定性的分析;以熒光染料rhodamineb為溫度熒光探針,建立了pdms微流控晶元上的溫度-熒光強度的關系公式,並利用matlab圖像處理工具箱構建出微流體溝道內的溫度色圖,對pdms微流控晶元的微流道溫度特性進行了分析,根據實驗結果,我們認為對于pdms微流控晶元來說,在進行需要外加電場作用的試驗時,外加電場不應超過400v cm 。Under these conditions the fibrinolytic activity of the supernatant can reach 820 urokinase units per milliliter broth. ba - dfe was purified from the supernatant of b. amyloiquefaciens dc - 4 culture broth by ammonium sulfate precipitation, ion - exchange chromatography on cm - and deae - sepharose fast flow, hydrophobic interaction chromatography on phenyl sepharose 6 fast flow and gel filtration on sephadex g - 50. the purified enzyme displayed thermophilic, hydrophilic and strong fibrinolytic activity
通過硫酸銨分級沉澱、 cm - sepharosefastflow和deae - sepharosefastflow離子交換層析、 phenylsepharose6fastflow疏水層析和sephadexg - 50凝膠過濾等方法,從解澱粉芽孢桿菌dc - 4的發酵液中分離純化出電泳純的ba - dfe 。Ion mobility spectrometry ( ims ) is a gas phase electrophoretic technique with the advantages of speed, low detection limits and low cost
摘要離子遷移譜技術是一種氣相環境下的電泳檢測技術,具有快速、靈敏、運行成本低等特點。By using a carefully optimized form of iontophoresis, we are able to deliver specified profiles of neurotransmitter directly to single synapses
並經由謹慎的利用離子電泳法,我們可以直接傳送特定型式的神經傳導物質到單一突觸。Standard test method for determination of dissolved inorganic anions in aqueous matrices using capillary ion electrophoresis and chromate electrolyte
用毛細管離子電泳和鉻酸鹽電解液法測定含水物質中溶解的無機陰離子的標準試驗方法分享友人