離心后沉澱 的英文怎麼說

中文拼音 [xīnhòuchéndiàn]
離心后沉澱 英文
centrifuged deposit
  • : Ⅰ動詞1 (離開) leave; part from; be away from; separate 2 (背離) go against 3 (缺少) dispens...
  • : Ⅰ動詞1 (沉沒; 墜落) sink 2 (沉下 多指抽象事物) keep down; lower 3 [方言] (停止) rest Ⅱ形容...
  • 離心 : 1 (不是一條心) be at odds with the community or the leadership2 (離開中心) centrifugal; eccen...
  • 沉澱 : 1 (沉澱過程中析出的物質) sediment; precipitate; sedimentary accretion; precipitation; (doposit...
  1. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞,,上清和進行sds一page電泳分析2 .樣品處理提取包涵體,變性,加人用pbs平衡過的以utal腸onesepb抓脫4b ,室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ,決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積,用預冷的1xpbs清洗cldtathionese戶, se4b ,得到50 %的基質
  2. After washing with reagent ( 50mmol / l ph8. 0 tris - hcl, 100mmol / l nacl, 0. 5mmol / l edta, 2mol / l carbamide, 0. 2 % triton x - 100, 0. 2 % doc ), ultrasound crushing ( 200 w, 150 times, 3 seconds per time, spacing 3 seconds ) and freezing - melting methods, we got hng fusion protein and m - insulin fusion protein with purity of above 80 %. 4. radioimmunoassay result shows that the radioimmune activity of of m - insulin fusion protein is 0. 5 unit per litre bacterial liquid

    3 .表達產物初步純化仁gly 」一flumanin融合蛋白和人胰島素突變體融合蛋白包涵體的洗滌方案為: lj ]體溶於含zmol , / l尿索洗滌液,超聲( 200w 、巧o次、 3秒/次、間隔3秒) , 4 、 i000orpm15min用洗滌液充分重懸,一2 ( )凍存過夜,次日融化4 、 10000rpm15min ,取上清,即得到初步純化的融合蛋白,可去除土要的雜蛋白,融合蛋白溶解于上清液中,純度達到80 %以_ 1 : 。
  3. Report accidents and incidents promptly to the responsible person. these include spillage of infectious materials and chemicals, breakage of tubes during centrifugation, cuts, burns and contamination of non - intact skin or mucous membrane by potentially hazardous materials

    發生意外或事故須盡快向負責人員報告,包括傳染物料及化學品濺溢、時試管破裂,以及破損皮膚或黏膜被可能具危險性的物料割傷、燒傷或沾染。
分享友人