離析酶 的英文怎麼說
中文拼音 [líxī]
離析酶
英文
macerozyme-
Studies of the chitosan microspheres prepared as affinity chromatography carrier and used for purifying thrombin from bovine blood plasma
殼聚糖微珠作為親和層析載體的制備及其用於分離牛凝血酶的研究Analysis of copper binding protein by sds - page, three main protein bands observed. the main bands were digested by lysyl endopeptidase and isolate different peptides by hplc. 4
Sds page分析得到3條主要蛋白帶，剪下這三條帶進行膠內賴氨酸內切酶的消化，通過高效液相色譜分離肽段，選擇性進行肽段的氨基酸序列測定。The availability for classification of hemiptera insects by using the esterase isoenzynes, microorganisms and digestive enzymes characters has been studied. the results are as follows. 1. there were differences in the electrophoretic pattern of the bugs
本文採用酯酶同工酶電泳、鏡檢與分離培養及消化酶分析對蝽類昆蟲酯酶同工酶、體內微生物及主要消化酶類進行了初步研究，結果如下： 1酯酶同工酶電泳表明：蝽類昆蟲酯酶同工酶譜存在明顯的差異，特徵酶譜重復性和穩定性較好。Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well
通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page
將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。This enzyme was different with the ones reported in the past. a phosphatase was isolated from the chloroplast thylakoid membrane of ipomoea aquatica, by nacl extration, ammonium sulfate precipitation, ion - exchange chromatography and hydrophic chromatography through butyl - toyopearl 650m column
使用nacl抽提、硫酸銨分步沉澱、離子交換和butyl - toyopearl650m疏水柱層析等方法，從蕹菜葉綠體類囊體膜中分離純化到一種蛋白磷酸酯酶。The separation of nattokinase in leavening agent
發酵液中納豆激酶鹽析法分離的研究In this article the methods for purification and assay for pectic enzymes were reviewed with 28 references
本文對果膠酶分離純化手段及其分析方法進行了綜述。The purification and assay methods for pectic enzymes are differed with various strains
由於不同菌種產生的果膠酶成分復雜程度不同，分離純化手段和分析方法也不相同。The profile of microbial community structures in different deep sea sediments was evaluated and the interaction between microorganisms and environment was analyzed by culture - independent molecular phylogenetic methods. psychrophilic and psychro - tolerant bacteria were cultured and used for screening the cold - active enzymes
此外，本文還對採集自太平洋的深海沉積物和南極、北極區域的樣品進行了低溫微生物的分離培養及低溫酶的篩選與性質分析。Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus
本研究利用線蟲誘導下owvt - 1菌株液體發酵，通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶，生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用，同時測定了該酶理化特性，酶活力在75附近酶活力最高，隨著ph的增加酶的穩定性升高，與膽堿酯酶具有相似的ph曲線，對特異性底物aape （ suc - ala - ala - pro - glu - pna ）具有作用， ssi和ci - 2抑制該酶的活性。Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。Medium experiments were arranged under uniform design, and then an optimum medium was got accordingly. the culture liquid was centrifugalized at 3, 500r / min for 30min, then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others
通過硫酸銨分級沉澱、 deaesephadexa - 50陰離子交換凝膠層析和sephadexg - 75凝膠柱層析對發酵液進行分離和純化，並得到電泳純的酶。According to the chemical characteristics of organophosphorus pesticide and the principle of organophosphorus pesticide inhibit plants cholinesterase activity, based on determined enzymatic reaction conditions, the separation effect of different developing agent system on 11 organophosphorus pesticides by the means of thin - layer chromatography were researched, followed 10 qualitative analysis methods of organophosphorus pesticide residues were established
摘要根據有機磷農藥化學特性及其對植物膽堿酯酶抑制的原理，在確定酶促反應條件的基礎上，考察了不同展開體系對11種有機磷農藥的薄層層析分離效果，建立了10種有機磷農藥殘留的定性分析方法。The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides
進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。Then enzyme was purified with a deae - cellulose ( 5. 5x50cm ) column, a toyopearl hw - 65 ( 5. 5 x 50cm ) column and a sephadex g - 200 ( 5. 5 x 80cm ) column. finally, the enzyme was purified for 10 folds with the recovery of 17. 4 %. page showed a single band for the purified creatinase
3 、肌酸水解酶的提純酶在硫酸銨飽和度為40 80之間完全沉澱，先後經過deae - cellulose離子層析柱、 toyopearlhw - 65疏水層析柱、 sephadexg - 200分子篩層析柱層析，最終使酶提純10倍，最終得率為17 . 4 。Calmodulin - dependent cyclic nucleotide phophodiesterase was prepared from bovine brain by two - step deae - cellulose column chromatography
摘要通過兩次離子交換柱層析，從牛腦中制備鈣調素依賴性的環核苷酸磷酸二酯酶。Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution
3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。Mm ). mg2 +, mn2 +, zn2 +, fe2 +, fe3 +, cu2 + have enabled effect on enzyme activation and edta produce a strong inhibitory effect on the enzyme. embranch amino acid have no effect on the enzyme
在離子交換柱層析中，採用不同的ph值及不同類型的緩沖液對純化條件進行優化，最終選擇了ph6 . 0mes緩沖液，並得到了酶蛋白洗脫點為0 . 24 0 . 32mol lnacl 。Molecular imprinting polymers ( mips ) have exhibited extensive application prospect in separation, purification, immunoassay, enzyme mimic, biomimic sensor, and other fields
分子印跡聚合物在分離提純、免疫分析、酶模擬以及生物模擬傳感器等許多方面顯示出廣泛的應用前景。分享友人