電轉化 的英文怎麼說

中文拼音 [diànzhuǎnhuà]
電轉化 英文
electrotransformation
  • 電 : Ⅰ名詞1 （有電荷存在和電荷變化的現象） electricity 2 （電報） telegram; cable Ⅱ動詞1 （觸電） give...
  • 轉 : 轉構詞成分。
  • 轉化 : 1. （轉變） change; transform 2. [化學] inversion; conversion
  1. The artificial antibacterial peptide - ceme gene was designed according to the codon with the highest frequency in pichia pastoris, then the ceme gene was integrated into the chromosome of pichia pastoris strain - gsl 15 by electroporation

    我們根據人工設計的新型抗菌肽ceme序列設計ceme基因，重組到酵母胞內表達型的整合載體phild2中，通過電轉化作用將ceme基因整合至酵母宿主gsll5染色體上。
  2. It was then cloned to the secreted vector - ppic9k and recombined successfully into the chromosome of pichia pastoris host strain - gsl 15 by electroporation

    通過電轉化作用該基因片段被成功地整合至酵母cs115的染色體上，經過甲醇誘導之後，該基因得到了分泌表達。
  3. The principles of laser technology, photo - electronic transformation technology and photo - electronic signal gathering technology were discussed in this paper, a new imaging laser threat warning device used in antagonizing laser - guiding weapon was designed and its main parts and circuit were described in detail

    本文闡述了激光技術、光電轉化技術以及光電信號採集技術的基本原理。根據來自激光制導武器的威脅，採用較為先進的光敏電荷耦合器件（ ccd ）和廣角遠心魚眼型透鏡的探測技術，以及幀存儲和幀減法技術。
  4. Dna and rna dot blotting revealed that the f gene was transcribed into mrna in the vero cells. there was expression of the f protein as shown by indirect immunofluorescent assay. the expression began at 48h post - infection and increased thereafter, as indicated by elisa

    將真核表達質粒pcdna3 - f高壓電轉化dam和phop基因雙突變的減毒鼠傷寒沙門氏菌zj111株（ zj111 / pcdan3 - f ） ，並直接轉染vero細胞，分別提取細胞總dna和總rna ， dig標記探針均可檢測到陽性雜交信號。
  5. To the discharge in the air gap, with the increase of applied voltage, the discharge area is larger, and the time interval is smaller, and discharge pulses frequency is higher

    對于間隙中的放電情況，施加電壓越高，放電區域越大，放電時間間隔越小，放電脈沖頻率越高;電壓進一步升高，放電轉化為穩定的輝光放電。
  6. The stable expression yeast was obtained by screening and was induced to ferment by methanol

    將ppicgk七質粒電轉化gslls酵母；通過篩選獲取穩定表達ctla4胞外區蛋白的表達菌。
  7. Three kinds of parasporal crystals of bipyramidal, cubical and embedded shapes were observed under an electronmicroscope

    建立了一個利用電轉化技術構建遺傳改良工程菌的新模式。 3
  8. Because of its high conversion efficiency, mature producing techniques and good reliability, solar cell was firstly used in space field

    單晶硅太陽能電池因其電轉化率高，製造工藝成熟，可靠性好而首先被用於航天領域。
  9. The transformation frequencies of exogenous plasmids in bmb171 were 7. 5 103 - 8. 1 108 folds as much as those in ybt - 1463, and stability of exogenous introduced in 8mb 171 was obviously higher than in ybt - 1463

    用pht3101等5種供體質粒電轉化bmb171的效率比電轉化ybt - 1463的效率顯著提高，而轉化導入的3種外源質粒在bmb171中的穩定性也明顯高於出發菌株ybt - 1463 。
  10. The very plasmid pbmb0474 was constructed. when the recombinant plasmid pbmb0474 was transferred into crystal negative bt strain bmb171 through electroporation, the expression of the fusion genes about 150kda - 160kda can be detected by sds - page. 4

    將pbmb0474電轉化到bmb171中得到蘇雲金芽胞桿菌重組菌， sds - page結果顯示重組菌可以表達大小約150kda - 160kda的gfp融合蛋白。
  11. The recombination expression vector, ppic9 - e2, was linearized by sal i and electroporated into p. pastoris gs115. recombinant p. pastoris gs115, designated gs115 - ppic9 - e2, was identified by pcr and the product of the pcr was analyzed by sequencing before its expression for csfv e2 protein

    重組表達載體ppic9 - e2經sal酶切線性化后，電轉化整合到畢赤酵母gs115基因組上，經pcr鑒定和pcr產物測序分析，陽性轉化子命名為gs115 - ppic9 - e2 。
  12. After pcr checking and electro - transformation plasmid from c. elegans into dh5a, isolating plasmid from dhsct, digesting them with restriction enzymes and dna sequencing, six cdna fragments, which protein products can interact with rapgap, from cdna library were got

    將pcr擴增陽性及或lacz強陽性質粒電轉化入dhsa細菌，提取質粒后酶切、測序。發現有6個來源於celegqnscdna文庫的dna片段編碼的蛋白可以與ra帥ap相互作用，其中兩個為rapgap 。
  13. In this article, the advanced structure of hybrid peptide mae is predicted with software. the fused gene mae - intein - cbd is amplified by pcr with the template of plasmid ptyb2, and then it is cloned into expression vector plasmid ppic9k. after verified by restriction enzyme analyzing and sequencing, the vector is transferred into the eukaryotic host ( yeast pichia

    並以已構建的載體ptyb2 - mae為模板，通過pcr擴增出融合基因mae - imein - cbd ，將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
  14. London - listed renesola does producewafers, but focuses onmonocrystalline wafers which are less effectivethan the multicrystallineingots and wafers that ldk produces

    在倫敦上市的浙江昱輝也生產矽片，但只是單晶硅矽片，這比江西賽維生產的多晶硅矽片和硅錠，光電轉化率要低。
  15. In this article, the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression. adopted a new strategy, multiple copy gene is ligated in the same direction. and then it is cloned into expression vector plasmid ppic9k

    本文根據genebank登錄的氨基酸序列，同時考慮構建和表達的需要，化學合成了misgurin基因和接頭，採用一種新的策略，在體外將基因多拷貝同向串連，並將其克隆于表達質粒ppic9k中，通過鑒定並測序正確后，電轉化真核表達宿主? ?畢赤酵母菌株gs115 ，通過營養缺陷型培養基篩選重組子，再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
  16. First, variable speed constant frequency wind energy convertion theory is described in this paper, then presents a review on the development of wind turbines control and the main types of generator and static converters used to interface variable speed wind turbines to the electric grid. then discuss main circuit constructure and advantage of direct drive wind energy conversion system and introduce pitch - control method for wind power traction and electric power stability. simply aerodynamic characteristic of the turbine is analysised and permanent magnet synchnonous generator math model is established. to convert the variable frequency electricity into utility grid, back to back four - quadrant pwm - vsi is used and three typies of control strategy is presented to capture the maximum wind energy and transmit energy. then simulation is implemented to test the control strategy. in the following chapter a simple ac - dc - ac converter with a dc - dc boosting chopper is proposed to transmit the wind energy into electricity energy and two control strategy is presented

    建立了永磁電機和變流器的數學模型，針對雙pwm變頻器的特點提出了三種控制策略對變流器進行控制，通過變流器交-直-交的變換，將發電機發出的變頻變幅值交流電轉化為可用的恆定頻率的交流電，通過pwm調治能使其輸出功率因數為一，並且該控制系統功率因數為可調，能在特殊情況下同電網交換一定的無功功率，並通過對變流器的控制實現了最大風能俘獲的功能。最後採用matlab / simulink進行了模擬，取得了良好的模擬效果。在風力發電系統中，採用先進的最大功率俘獲演算法，能有效的從風中獲得最大的能量。
  17. It is important work for scientific researchers to improve utility ratio of solar energy, especially to improve conversion efficiency of solar cells, there are many ways which can be used to improve it, depositing antireflection films on solar cells is the most doable one, and can debase the cost of solar cells

    提高太陽能的利用率，尤其是太陽電池的光電轉化率是科研工作者研究的一個重要方向。對于提高太陽電池的光電轉換效率的方法很多，但比較可行又能降低太陽電池成本的方法是在太陽電池表面形成一層減反射薄膜，以減少太陽電池表面對陽光的反射損失。
  18. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  19. The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively

    三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變， pcr產物與ppic3 . 5k連接，轉化大腸桿菌，獲得的混合質粒電轉化畢赤酵母gs115 ， omm平板篩選適于低溫或對熱穩定的重組子，篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ，其最活反應溫度為45 ，比野生型高出5 ； 40處理30min殘留活性為56 ，大大高於野生型的6 ；初始ph7 . 3528條件下培養72h ，發酵上清酶活為325u / ml 。
  20. The maximal incident photon - to - current conversion efficiency was 48. 32 % at the wavelength 560nm

    電池的單色光光電轉化率在波長560nm處達到最大，為48 . 32 。
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