非基因的 的英文怎麼說
中文拼音 [fēijīyīnde]
非基因的
英文
extragenic-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。To study on structure and inheritance of rh d gene interaction between gene expression of rh d and rh c / e and influences on rh d gene expression of inserts and rh box - methods : 20 pairs of oligonucleotide specific primers for exon, intron 2 4, insert and rh box of rh d, rhc / e gene were designed and composed the polymerase chain reaction - sequence specific oligonucleotide primer ( pcr - ssp ) was used to amplify the rh c / e gene, rh d gene, exon, intron, insert and rh box in 106 samples of unrelated individuals and 7 han nationality ancestries and 5 wei nationality ancestries whose proband were rh d - negative
目的:觀察中國漢族非血緣關系隨機個體、漢族及維吾爾族家系rh血型的c e基因、 d基因外顯子、內含子、插入片段以及rhbox ,研究rhd基因結構及遺傳規律, d基因表達與c e基因的關聯,以及插入片段和rhbox對d基因表達的影響。3. the effect of sporulation - independent promotor on toxicity of natural strain in order to study the effect of sporulation - independent promotor ( p3a ), p3a was spliced with the cry1c gene, then inserted into the shuttle vector pht304, and then recombinated plasmid pbmb827 was obtained. after transferring pbmb827 into strain ybt - 1520, it was surprising that the transformants had almost no potency against all lepidopteran larvae tested
3非依賴芽胞形成icp的cry3a啟動子( p _ ( 3a ) )對野生菌株特性的影響帶p _ ( 3a )和cry1c基因的重組質粒pbmb827轉入ybt - 1520 ,轉化子對所測昆蟲的毒力下降非常明顯,芽胞和晶體也很難脫落。It is applicable to coinject two or three genes whose expression products have no antagonistic action. 4. coinjection is a simple gene transfer method which saves time and money
非桔抗基因(轉移基因的表達產物互不抑制)的共轉移(顯微注射法)是一種既經濟、又有效的方法。They say that will help the search for genes and non - genetic factors that explain why american blacks run elevated rates of such diseases as high blood pressure, prostate cancer ( news - web sites ), obesity, asthma and diabetes
他們說這樣會有助於研究為什麼美國黑人在像是高血壓,前列腺癌,肥胖,氣喘跟糖尿病逐年升高的比例中基因跟非基因的因子。In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv
本研究採用脂質體轉染方法,將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞,經6輪蝕斑克隆、篩選、表達, pcr鑒定和dot - elisa檢測,證明該重組病毒已構建成功,並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。Most snps lie in the non - coding regions of the genome, so using them to make inferences about genes will in most cases infringe the patents
大部分snp都存在於整組遺傳基因的非代名層里。因此,利用它們來作遺傳基因的推理在多數情況下都會侵犯專利權。In our laboratory, a unique mutation detection system using a shuttle vector plasmid has been established to demonstrate that a low concentration of mnng ( 0. 2 m ) can induce nontargeted mutation in mammalian cells : the mammalian cells were exposed to 0. 2m mnng for 2. 5h, then a shuttle plasmid pz189 carrying supf trna gene was transfected into cells after 24h culture. we found a 5 - fold higher mutation frequency of the plasmid replicated in pretreated cells than the spontaneous mutation frequency of the plasmid replicated in control cells. this kind of mutation did not occur immediately after mnng exposure
我們實驗室曾用一特殊的突變檢測系統,直接證明dna損傷劑可在哺乳動物細胞誘發非定標性突變:首先用低濃度( 0 . 2 m )的短壽烷化劑mnng (半壽期為1 . 1hr )處理細胞2 . 5h后,繼續培養24h ,將重組有用作突變檢測的靶基因supftrna基因的穿梭質粒pz189轉入細胞復制,發現在未受致癌物直接攻擊的穿梭質粒中有較自發突變率高5倍以上的靶基因突變。And there is genotypic resistance means mutations are seen, in divided sequence, and gpt or alt levels are high, there is genotypic resistance
除非你發現基因型的變異,也就是變異出現,基因的序列改變,穀草轉氨酶或者谷丙轉氨酶高,說明基因型耐藥出現。Also, very importantly, the biggest application of this technology doesn ' t need gene therapy at all, because the cells that need to be given the microbial genes are macrophages, special white blood cells, which come from the bone marrow
再者,非常重要的是,這項技術的最大應用,完全不需要基因療法,因為需要給予微生物基因的細胞,是來自骨髓的巨噬細胞(特殊的白血細胞) 。According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr
然後根據dsrna設計原則,結合nssb基因的序列特徵,藉助生物信息學軟體設計了針對nssb基因的sirnas ,並交由公司化學合成;電穿孔法轉染上述穩定轉染的細胞克隆,同時分別以非特異的sirnas轉染組和空白轉染組為對照, dapi染色后通過熒光顯微鏡和內標化rtpcr檢測,初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。In e coli, dna polymerases are key enzymes involved in two distinguished pathways contributing to untargeted mutagenesis. replication of dna by pol v ( umuc ), in the presence of umud1, reca and single strand binding protein ( ssb ), is highly mutagenic and exhibits a predominant mutation pattern of base transversion. another error prone polymerase involved in untargeted mutagenesis is pol iv, encoded by dinb gene
在umud ' , reca和單鏈結合蛋白ssb的協助下, polv ( umuc )能在單鏈模板上催化dna合成並產生高頻率的以堿基顛換為主要形式的突變;另一個與非定標性突變有關的易誤dna聚合酶是pol ,為dinb基因的編碼產物。Meanwhile, base composition in rrna genes, the secondary structrues of trna and the potential stem - loop structure in the noncoding region were also analysesed in the present study
同時分析了d1na基因的堿基組成、 trna的二級結構、非編碼區潛在的莖環結構。One of the main lines of evidence for the “ out of africa ” hypothesis, as it is usually known, is that the most genetically varied human populations are in that continent ? particularly in the south and east of it
眾所周知, 「走出非洲」假說的主要證據是:大多數不同基因的人就在那個大陸? ?尤其是南部和東部。The phylogenic relation and similarity of the gene is full length of lfy - like gene, which is 99 % similar both in nucleic acid sequence and amino acid sequences with that of ginlfy f rom the female. compared with the ginlfy of female, the gene from the male tree is deficient in 3 bp, and mutantion sites exists on the varied region of lfy - like
該基因與文獻報道的銀杏雌株leafy基因相比,核苷酸序列同源性高達99 ,蛋白質序列同源浙江大學博士學位論文摘要性達99 ,缺少了3個核昔酸,突變均在植物leaf 』 y基因的非保守區內。Location of the hbrp gene in the chromosome according to stanford g3 rh panel, a pair of primers are designed in the 3 " - utr region of the gene which is to be localized and human genome dna is amplified in advance whose products are examined by electrophoresis. then g3 panel is amplified and this process is repeated twice. the pcr result is sent to stanford human genome center ( shgc ) http : / / www - shgc
Hbrp基因的染色體定位使用stanfordg3rhkadiationhybrid )嵌板,在所要定位基因的3 』非翻譯區( untranslatedregion , utr )設計引物,預先擴增人基因組dna ,電泳檢測產物大小,繼之擴增g3rhpanel ,重復兩次,將pcr結果輸人斯坦福人類基因組中心( shgc )的主頁( http : 、 shgcYellow bettas are also called non - red ( nr ) bettas. this mutated gene causes the alteration of red pigment to yellow
黃斗魚又稱為非紅斗魚,基因的突變讓紅色素轉黃。Since the state of gene should not be observed obviously, it is often to study the state of gene through the level of the protein expressed by the gene in cells
這樣原來研究的確定論非線性模型就轉變成了含噪聲的朗之萬方程( le ) 。由於實驗是無法測出基因的狀態是處于開還是關。One is as signal transducers outside of the nucleus, named nongenomic action. the other is as transcription activators in the nucleus, named genomic action. the two ways may coexist, cooperate and coregulate in the development and maturation of oocyte
類固醇激素受體在卵母細胞中可能通過兩種途徑發揮作用,一種是存在於胞質中,作為信號轉導因子的非基因組調控機制;另一種是存在於核中,作為核轉錄活性因於,改變基因轉錄活動的基因組調控機制。This hypothesis provide a new thinking on the action of steroid hormone on neurons, and is both a challenge and a supplement to the traditional genomic theory, which held that the action of steroid hormone is solely mediated by its intracellular cytosolic receptor within the cell nucleus
年代首次在國際上提出糖皮質激素作用於神經元的快速、非基因組機制或膜受體假說。這是對傳統崽體激素基因機制或細胞學說的挑戰與補充,受到國際學術界的高度評價,曾應邀在第分享友人