顯微培養 的英文怎麼說
中文拼音 [xiǎnwéipéiyǎng]
顯微培養
英文
microculture-
Under mixed culturing conditions, it was observed that bacterial number rapidly incre ; ised soon after the lysing of host cells. on the contrary, while the non - host cyanobacterium ( i. e. anabaena flos - aquae ) was incubated in the mix culturing system, the breeding of the bacteria could be inhibited. it may be suggested from the result above that cyanophage could bring about the changes in microorganism populations
調查結果顯示， 19個採集的水樣中有6個含有裂解織線藻的噬藻體，而且水樣未經濃縮即能裂解宿主，說明噬藻體在淡水中分佈較廣泛，裂解性也較強；混合培養條件下的研究結果表明，噬藻體裂解宿主后，細菌數量快速增加，而當培養系統中有非宿主藻類存在時，細菌的增殖則受到非宿主藻的抑制，說明噬藻體可以顯著改變系統中微生物的種群結構。The availability for classification of hemiptera insects by using the esterase isoenzynes, microorganisms and digestive enzymes characters has been studied. the results are as follows. 1. there were differences in the electrophoretic pattern of the bugs
本文採用酯酶同工酶電泳、鏡檢與分離培養及消化酶分析對蝽類昆蟲酯酶同工酶、體內微生物及主要消化酶類進行了初步研究，結果如下： 1酯酶同工酶電泳表明：蝽類昆蟲酯酶同工酶譜存在明顯的差異，特徵酶譜重復性和穩定性較好。Methods the influence of fluoride on the rat first passage osteoblast were evaluated by histochemistry, enzymehistochemistry and electron microanalysis in vitro
方法應用組織化學、酶組織化學和電子顯微分析等手段觀察氟對體外培養的鼠第一代成骨細胞的影晌。Abstract : the early embryo developmental block is a common phenomenon in mammal when embryos are cultured in vitro. many studies of phosphorus, glucose, hypoxanthine and cytoplasmic factors on early embryo developmental block carried out by different methods such as morphology, biochemistry, molecular biology and micromanipulation have been reviewed. the merit and shortcoming were analyzed and the necessity of using simple or components limited media overcoming early embryo developmental block were also reviewed. media that have been shown effective in overcoming early embryo developmental block in mouse, rat, hamster, rabbit, pig, sheep, cattle and monkey were listed
摘要哺乳動物胚胎在體外培養中普遍存在早期發育阻滯的現象.對此，人們用形態學、生物化學、分子生物學、顯微操作等手段開展了磷酸、葡萄糖、次黃嘌呤和細胞質因素對早期胚胎發育阻滯的影響的研究.本文綜合分析了共培養系統的優缺點.說明了採用完全成分已知的培養液對進行有關研究的必要性.列出了有效運用於克服小鼠、大鼠、倉鼠、兔、豬、羊、牛、猴等動物早期胚胎阻滯的成分已知的培養液的名稱。With the aid of stereo microscope, optical microscope and scanning electron microscope, the periduim, spore and capillitium of all 8 species of myxomycetes, including field collections and agar - development fruiting, were observed and compared from apparent morphology, microstructure to ultra - structure
應用實體顯微鏡、光學顯微鏡和掃描電子顯微鏡，對培養的子實體和野生型子實體的囊被、孢子和孢絲等進行了一般和超微觀察及比較研究。Observe shapes and structures of the cultured and subcultured cells with phase contrast microscope and transmission electron microscope, and then explore the growth character of the cells of smg
2 .應用倒置相差顯微鏡、透射電鏡觀察培養細胞的形態結構，了解細胞的生長特性。Sds - page results showed that there was a clear target protein band in mut + recombinant supernatant after 48 hours of culturing, while a faint band only in muts recombinant after 72 hours. western - blotting result showed that there was no remarkable difference of yield between mut + and muts recombinants after 6 days induced. anti - virus activity tests revealed that culture supernatants of mut + and muts recombinants could inhibit tmv infection with high efficiency in the same concentration and there was no significant difference between them
結果表明，誘導培養48小時后， mut ~ +重組菌株表達產物在sds - page膠上顯現出清晰的目的蛋白帶，而mut ~ s重組菌株培養72小時才能顯示微弱的目的帶； western - blotting雜交信號強度表明，同樣培養6天的mut ~ +和mut ~ s重組菌株表達產物在表達量上沒有明顯差別。And other three cyanobacterial species in laboratory condition. however, the sterilized decomposed rice straw treated with a sterilizer at temperature 121 for 15min has no such inhibition effect. this indicates that the microbes associated with the decomposing rice straw possibly play an important role in producing and releasing the algal inhibitors from the straw. fresh rice straw, without decomposing treatment, exhibited no efficiency of algal inhibition. the present study has proved that the rice straw may have great potential of application to the algal blooming control
研究證明在有氧條件下經過一個月以上降解的稻草對銅綠微囊藻等4種實驗室培養的藍藻具有明顯的生長抑制作用。然而，滅菌的降解稻草121 ， 15min並沒有抑藻效果。這說明伴生微生物的降解作用對于稻草的抑藻因子的產生和釋放是必要的。After adding culture mediem of stably transfected jurkat cells to hepatocarcinoma cells, the binding specificity of the scfvs with hbsag was further confirmed by observation by fluorescence microscope, indirect immunofluorescence and flow cytometer analysis. prokaryotic expression plasmids ptat - ha - scfvs were successfully constructed
建系細胞培養上清與肝癌細胞作用后，經熒光顯微鏡觀察、間接免疫熒光及流式細胞儀檢測進一步確定表達的scfv融合蛋白具有與hbsag特異性結合的活性。Potato dextrose agar and grain medium were also used to identify fungi which were not determined by the primary culture. fungi were all secondarily cultured on sabouraud medium to observe the colony ' s texture, colour, growth rate, surface status and reverse pigment. the fungi should be examined by microscope to inspect their microscopic structure from 7th day to 21st day
使用的培養基有沙氏培養基、土豆培養基、真菌試驗培養基和5種種子培養基，連續培養4周，並隨時觀察菌落的色彩、生長速度、表面狀態、背面顏色等，並從第7天?第21天連續鏡檢以檢查真菌的顯微結構，綜合菌落形態和顯微結構，以確定真菌的種屬。We studied development mechanism by the distribution of microfilaments and actin mrna in cotton callus, healtny plants and abnormal plantlets. fitc - phalloidin as fluorescence probe was used to investigate the meristem of the cotton root, abnormal plantlets and callus that was unable to germinate into healthy plants
本研究選取正常棉花的根，已經培養了長時間不能分化出正常植株的棉花愈傷組織和棉花畸形苗為材料，採用石蠟切片，通過fitc -鬼筆環肽對材料微絲熒光染色，結合熒光顯微鏡觀察。By using inverted microscope, it was observed that dunaliella salina of different growth stages after the high osmotic shocks can live in the medium with nacl concentration between 0. 1m and 5. 0m, but its growth status and period showed differently. the optimal concentration for the growth of dunaliella salina was 0. 5 - 1. 5m, and this organism could stand a variety range of osmotic shock. enolase gene, the anti - adversity gene of d. salina, was cloned by modified degenerate pcr technique
通過倒置顯微鏡觀察生長在不同鹽濃度，不同生長時期，以及經不同滲透壓震動的鹽藻，四川大學博士學位論文發現其在o . im一5 . omnaci培養基中均能正常生長，但其生活狀態及生長周期有所不同，其最適生長naci濃度為0 . 5一1 . 5m ，還能適應各種高滲及低滲震動。The characteristics of this method are : a, directly counting cell number without the influence of the metabolic state of the cells ; b, discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c, less treatment step, and free - radioactivity ; d, high sensitivity and reliability. 2, using the above assay, immunofluorescent labeled technique, and flow cytometry, the pbmc proliferation, apoptosis, necrosis, cell cycle, activation, cytokines and membrane marker were detected. the results showed that the number of pbmc reduced, but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle, but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased
結合以上創建的方法和免疫熒光流式細胞術，用k562細胞株可溶性分泌物（上清）對外周血單個核細胞（ pbmc ）進行培養以模擬體內微環境，然後分別從細胞增殖、凋亡、壞死、細胞周期、活性、細胞因子和表面抗原表達等方面進行研究，結果發現用腫瘤上清培養的pbmc細胞數量下降明顯，但同時對其有激活作用，且呈劑量依賴性；細胞數的下降主要是由細胞壞死和凋亡引起的，腫瘤上清對細胞周期沒有阻斷作用，反而略有促進作用； t細胞亞群比例增加，並促進表達th1 、 th2細胞因子。Results the epithelial cells of ameloblastoma grew faster, with an irregular area around the cell mass and some small satellite - shaped cell mass
方法原代體外培養成釉細胞瘤細胞、牙源性角化囊腫、根端囊腫及口腔粘膜的上皮細胞和成纖維細胞，倒置光相差顯微鏡觀察。After inoculation, all strains were examined by microscopy, hyphae or cells of all strains were observed, but none was seen in negative comparison. in the same time, originally fungi were isolated again in sabourud which showed that these fungi could grow and reproduce in these animals, but if they can cause infection or not will be make sure with impressionable animals
回接后所有實驗菌種在顯微鏡下均可見有菌絲或菌細胞生長，而陰性對照組則未見生長，同時可以從沙堡氏培養基中再次分離得到該菌，說明這些菌種均可以在動物體內生長繁殖，但是否能真正引起感染，還需要進一步使用易感動物進行確認。Figure 2. b, on high - power magnification microscopy, the specimen showed a patternless tissue culture - like appearance with multiple mitoses and extraasated red blood cells
圖2b ，高倍顯微鏡檢查，標本顯示無圖案的「組織培養樣」表現，伴有大量有絲分裂和紅細胞外滲。Sections were stained by he and were observed under light microscope. ( 4 ) observation on cell - matrix complex with confocal microscope. cell - matrix complex was stained by fluorochrome cfda - am ( loug / ml, looul ) after 7 days incubation, the sample was scanned by confocal microscope to observe cell - growth in the matrix
細胞一多孔膜復合物的激光共聚焦顯微鏡觀察：取培養7天的細胞一多孔膜復合物，以10ug inl的熒光染料cfad am100ul染色，檄光共聚焦顯微鏡檢測激光激發的綠色熒光，掃描成像觀察活細胞生長倩況。N sources ( including organic and inorganic n tested ) and c sources tested could restrain methane oxidation. cellulose inhibited methane oxidation most weakly while the high concentration of methanol and glucose did dramatically, but the proper concentration of methanol could stimulate soil methane oxidation sharply. in the middle process of methane oxidation, addition of glucose could restrain methane oxidation shortly but the inhibition could be relieved about 5 days later when supplied again with enough oxygen
土壤微生物是甲烷氧化的主要生物類群，含水量對土壤甲烷氧化活性有明顯影響，過高或過低對甲烷氧化均具有抑制作用；氮源（包括有機和無機氮源）對甲烷氧化均有抑制作用；不同碳源對甲烷氧化的影響各異，纖維素對甲烷氧化抑制作用最小，而高濃度的甲醇、葡萄糖則對甲烷氧化具有強烈抑制作用；而適當濃度的甲醇可極大促進土壤對甲烷的氧化：在甲烷氧化過程中加入葡萄糖能迅速抑制甲烷氧化；在加入葡萄糖的同時保持瓶中充足的氧氣，則這種抑制作用可以在重新培養一定時間后得到解除。Mixed lymphocy te culture and determination of tendon cell activity suggested that the antige nicity of dgua - cultured and cryopreserved tendons was significantly attenuated and the cultured and cryopreserved tendons were viable
趾主動屈曲功能，大體、顯微及超微觀察，羥脯氨酸含量測定及生物力學分析等結果均顯示，培養及冷凍處理的異體移植肌腱與自體移植肌腱差異無顯著性意義。With bacterial cgc as main subject, the tests had been done to elucidate mechanism of self - organization for macroscopic rhythmic structure. the dynamics of cgc forming was observed by special techniques of waving culture and microscopic culture ; the differences in outer structure of cell wall and flagella number had been observed by atomic force microscope scanning ; integrity of cell wall was examined under tem ; outer membrane protein was analysed by sds - page and various substance and factors for cgc formation were determined
採用特殊的波動培養和顯微培養技術觀察潛生體形成動態；應用原子力顯微鏡掃描，比較細菌潛生體與繁殖體在細胞壁外層結構和鞭毛數量的差別；用透射電鏡觀察細胞壁完整性，以十二烷基硫酸鈉?聚丙烯酰胺凝膠電泳分析外膜蛋白的改變，並通過實驗分析多種物質和因素對潛生體形成的影響。分享友人