顯微熒光術 的英文怎麼說
中文拼音 [xiǎnwéiyíngguāngshù]
顯微熒光術
英文
microfluorometry- 顯 : Ⅰ形容詞1 (明顯) apparent; obvious; noticeable; evident 2 (有名聲有權勢的) illustrious and inf...
- 熒 : 形容詞[書面語]1. (光亮微弱的樣子) glimmering 2. (眼光迷亂; 疑惑) dazzled; perplexed
- 光 : Ⅰ名詞1 (照耀在物體上、使人能看見物體的一種物質) light; ray 2 (景物) scenery 3 (光彩; 榮譽) ...
- 術 : 術名詞1. (技藝; 技術; 學術) art; skill; technique 2. (方法; 策略) method; tactics 3. (姓氏) a surname
- 顯微 : microadiography
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By compressing a monolayer film, the coexistence of liquid condensed ( lc ) and liquid expanded ( le ) phases can be reached. the transition from le to lc is usually regarded as a first - order one, so the theory of crystallization can be applied. in this article we review our recent studies on the growth of lc domains in the le - lc coexistence region driven by the illumination of a fluorescent microscope. the mechanism of this unusual 2d domain growth phenomenon is discussed. the formation of faceted, dendritic and fractal - like domains as well as the evolution and the transition of these patterns are investigated
當處于氣液界面的類脂類化合物的單分子膜被壓縮時,隨著分子間距的縮小,單分子膜將經歷一系列相變過程.通過熒光顯微術可以觀測到新相的成核和生長過程.由於單分子膜的二維特性,該系統中的實驗觀測對于檢驗和發展二維界面生長理論尤為重要.本文總結了近年來本課題組與相關單位合作,在單分子膜系統中發現的實驗現象以及對其生長機制的系列研究.內容包括對單分子膜系統中的成核、界面穩定性、枝晶生長、形態演變等的觀測和分析Abstract : by compressing a monolayer film, the coexistence of liquid condensed ( lc ) and liquid expanded ( le ) phases can be reached. the transition from le to lc is usually regarded as a first - order one, so the theory of crystallization can be applied. in this article we review our recent studies on the growth of lc domains in the le - lc coexistence region driven by the illumination of a fluorescent microscope. the mechanism of this unusual 2d domain growth phenomenon is discussed. the formation of faceted, dendritic and fractal - like domains as well as the evolution and the transition of these patterns are investigated
文摘:當處于氣液界面的類脂類化合物的單分子膜被壓縮時,隨著分子間距的縮小,單分子膜將經歷一系列相變過程.通過熒光顯微術可以觀測到新相的成核和生長過程.由於單分子膜的二維特性,該系統中的實驗觀測對于檢驗和發展二維界面生長理論尤為重要.本文總結了近年來本課題組與相關單位合作,在單分子膜系統中發現的實驗現象以及對其生長機制的系列研究.內容包括對單分子膜系統中的成核、界面穩定性、枝晶生長、形態演變等的觀測和分析In this paper, wheat cultivars lovrin 10 ( resistant ) and 5389 ( susceptible ) were selected as materials in this system. the mesophyll protoplasts of wheats ( mpw ) were isolated using cellulase and pectolase digestion. by the indirect immune fluorescent labeling, microtubules ( mts ) pattern in mpw were showed clearly under the confocal laser scanning microscope ( clsm )
本試驗以抗(洛夫林10 ) 、感( 5389 )不同的兩小麥( triticumaestivuml . )品種為材料,採用酶解法制備小麥葉肉原生質體,利用間接免疫熒光方法結合激光共聚焦掃描顯微技術對小麥葉肉細胞原生質體的微管骨架進行了清晰的標記,並探索了微管骨架標記的影響因素。The characteristics of this method are : a, directly counting cell number without the influence of the metabolic state of the cells ; b, discrimination of target cells from effector cells in cell - mediated cytotoxicity assay ; c, less treatment step, and free - radioactivity ; d, high sensitivity and reliability. 2, using the above assay, immunofluorescent labeled technique, and flow cytometry, the pbmc proliferation, apoptosis, necrosis, cell cycle, activation, cytokines and membrane marker were detected. the results showed that the number of pbmc reduced, but the activity of pbmc increased dose - dependently ; the reduction of cell number resulted from necrosis and apoptosis ; the supernatant of k562 cell lines were not able to block the cell cycle, but to promote it ; the ratio of t cell subset and the expression of thl and th2 cytokines increased
結合以上創建的方法和免疫熒光流式細胞術,用k562細胞株可溶性分泌物(上清)對外周血單個核細胞( pbmc )進行培養以模擬體內微環境,然後分別從細胞增殖、凋亡、壞死、細胞周期、活性、細胞因子和表面抗原表達等方面進行研究,結果發現用腫瘤上清培養的pbmc細胞數量下降明顯,但同時對其有激活作用,且呈劑量依賴性;細胞數的下降主要是由細胞壞死和凋亡引起的,腫瘤上清對細胞周期沒有阻斷作用,反而略有促進作用; t細胞亞群比例增加,並促進表達th1 、 th2細胞因子。The customers should provide the gene expression plasmid which has been done the fluorescence labelling. we will provide the detail reports and microscopical photos
技術服務完成後向客戶提供詳細的實驗報告和熒光共聚焦顯微鏡照片。The customers should provide the gene expression plasmid which has been done the fluorescence labelling. we will provid the detail reports and microscopical photos
技術服務完成後向客戶提供詳細的實驗報告和熒光共聚焦顯微鏡照片。The composition, structure, and properties of the as prepared composite films have been characterized in detail by uv - vis, ftir, and x - ray photoelectron spectra, ellipsometry, scanning electron microscopy, atomic force microscopy, transmission electron microscopy, fluorescence spectroscopy, and standard four - probe technique
採用uv - vis光譜、 ftir光譜、 x -射線光電子能譜、橢圓光度法、掃描電子顯微鏡、原子力顯微鏡、透射電子顯微鏡、熒光光譜和標準四探針技術對所制備的納米復合膜進行了組成、結構和性能表徵。Here we use fluorescently labeled albumin, together with the powerful technique of intraital 2 - photon microscopy to show that renal albumin filtration in non - proteinuric rats is 50 times greater than preiously measured and is followed by rapid endocytosis into proximal tubule cells ( ptcs )
我們使用熒光標記的白蛋白以及強大的活體內雙光子顯微鏡技術,在不伴有蛋白尿的大鼠中發現其腎臟濾過的白蛋白量較以前文獻報道的大50多倍,這些蛋白隨即又迅速被近曲小管細胞以內吞形式攝取。Laser scanning confocal microscope combined with fluorescence probe and fluorescence resonance energy transfer techniques has become a very effective tool of researching the behavior of massive molecules in living cells
摘要激光掃描共聚焦顯微鏡結合熒光探針以及熒光共振能量轉移技術,已成為近年來應用在活細胞中研究大分子行為的一種非常有效的研究工具。The phase structure of different cu - fe thin films were studied by using grazing incidence x - ray analysis ( gixa ). the texture and residual stress of different cu - fe thin films were measured by scan of x - ray diffraction ( xrd ) and 2 scan with different. the thicknesses of different thin films were characterized by means of small angle x - ray scattering ( saxs ) technique. by using atomic force microscope ( afm ) measured surface roughness of thin films. the component of different thin film was characterized by energy disperse spectrum ( eds ) and x - ray fluorescence ( xrf ). the magnetic properties of cu - fe thin films were measured by means of vibrating sample magnetometer ( vsm ). in addition, the giant magnetoresistance ( gmr ) effects of different films were also measured. the original resistance of the film fabricated by a direction - current magnetron sputtering system is directly affected by bias voltage
利用掠入射x射線分析( gixa )技術對不同cu - fe薄膜的相結構進行了研究;利用xrd掃描及不同角度的2掃描對薄膜進行了結晶織構及殘余應力分析;運用小角x射線散射( saxs )技術測量了薄膜的厚度;採用原子力顯微鏡( afm )觀察了薄膜的表面形貌;運用能量損失譜( eds )及x射線熒光光譜( xrf )對薄膜進行了成分標定;使用振動樣品磁強計測量了不同cu - fe過飽和固溶體薄膜的磁性能;最後利用自製的磁阻性能測試設備測量了真空磁場熱處理前後不同薄膜的巨磁阻值。Fluorescence microscopy a form of microscopy in which fluorescent probes are added to the material being investigated
熒光顯微術:用來觀察研究插入熒光探針后的物體的一種顯微技術。Some methods such as image cytophotometry, fluorescence microscopic imaging, infrared ( nearinfrared ) and raman microscopic imaging, magnetic resonance imaging and scanning probe microscopic imaging are discussed emphatically, and the conceivable direction in the future has been suggested
重點討論了圖像細胞光度測量、熒光顯微成像、紅外(近紅外)與拉曼顯微成像、磁共振成像和掃描探針顯微鏡成像等技術,並展望了單細胞成像的發展前景。If we consider the whole cereal plant as two parts, leaves and non - leaf organs, then it could be proposed that improvement of wheat yields can be obtained by improving the partitioning of assimilates of non - leaf organs ( frey - wyssling et al, 1959 ; sharma - natu et al, 1994 ). although structure and function of leaves received considerable attention, those of non - leaf organs are poorly documented
本論文以小麥、水稻等幾種重要經濟作物為材料,應用透射電鏡、掃描電鏡、激光共聚焦顯微鏡以及氧電極,低溫熒光等生理技術手段,對作物非葉器官的結構和主要生理特性進行了較全面和系統的研究。Transfection of antisense pcdna3 - doc - 1r plasmid and testing influence on cell growth we have transfected sense and antisense pcdna3 - doc - 1r plasmids into mouse nih3t3 cells respectively, then transplanted cells which were in logarithm growth phase into 24 orifice plates, sampled one time per 24hs, every time chosen three orifices " cells
另將pcmv一cdkz 、 pflag一doc一ir質粒轉染hela細胞中,通過免疫熒光顯微技術,尋找doc一ir和cdkz兩種蛋白細胞內原位結合的證據。實驗結果1 .小鼠doc一ir基因鑒定小鼠doc一1redna編碼序列長為92obp , orf編碼127個氨基酸。Changes in h2o2 generation in guard cells of vicia faba induced by aba were measured by using fluorescence probe, 8 - hydroxypyrene - l, 3, 6 - trisulfonic acid ( hpts ). examination of epidermis peel was performed using a laser scanning confocal microscope ( lscm ) and spectrofluorometer, set to an excitation light of 405 nm and an emission light of 512 ran
以蠶豆葉片下表皮為材料,將熒光探針hpts導入蠶豆氣孔保衛細胞內,利用熒光光譜和激光共聚焦顯微鏡技術,檢測了aba誘導蠶豆氣孔關閉過程中h2o2的產生。In order to investigate the role of mannose receptor ( mr ) of human sperm, the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography. the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope, respectively. the mice were immunized with pmr and the antiserum was raised. after capacitation and induction of the acrosome reaction, the human spermatozoa and oocytes were incubated with the antiserum. then the sperm penetration assay was undertaken
為了進一步探討人精於mr在精卵融合中的作用,本文採用改良后的甘露糖-瓊脂糖凝膠親和層析法分離純化人精子mr ,並將提純的人精子甘露糖受體( purifiedmannosereceptor , pmr )作用於去透明帶的金黃地鼠卵母細胞,運用透射電子顯微鏡技術和羅丹明偶聯的兵豆凝集素( tritc - lca )免疫熒光標記技術觀察pmr對卵子的影響。H2o2 generation in guard cells was examined by laser scanning confocal microscopy based on fluorescence probe h2dcfda. the fluorescence intensity in guard cells of wild type and ios5 was essentially the same before treatment
以h2o2熒光探針h2dcfda結合激光掃描共聚焦顯微技術直接檢測了保衛細胞內的h2o2產生情況。未加任何處理之前,野生型與los5保衛細胞內熒光強度幾乎相等。This new method will bring significant developments in studying the principles of stomatal movement, and other quick movement in plants, c ) guard cells are incubated with ph dependent fluorescent chemical probe " bcecf am " and excited at 488nm, the fluorescent emission ratio method ( 520nm / 640nm ) is employed with laser scanning confocal microscopy, about 0. 4 ph unit increase in guard cell vacuoles is observed during stomatal closure that is induced by aba
本發展為保衛細胞與其它小細胞液泡的進一步研究提供了新思路。 c )本工作通過激光共聚焦顯微術配合ph熒光探針bcecfam的單激發( 488nm )雙發射的熒光比值法( 525nm 640nm )觀察到,用aba處理的表皮條上的開放態氣孔在關閉過程中其保衛細胞液泡內ph有一約0 . 4單位的上升。Furthermore, aba may activate multiple ca2 + flux in stomatal guard cells, triggering vacuolar k + ( rb + ) release. besides these known components, hydrogen peroxide is a ubiquitous second messenger which has been researched in plants. this paper showed aba - induced h2o2 generation in guard cells of vicia faba, and activation of ca2 + channels by h2o2
本實驗利用激光共聚焦顯微技術、膜片鉗技術以及熒光分光光度技術研究了aba誘導蠶豆氣孔保衛細胞產生h2o2 ,並可能是由nadph氧化酶催化產生的,同時也證明了h2o2是引起ca2 +內流的一個關鍵成分。The results of confocal laser scanning microscopy ( clsm ) and atomic force microscopy ( afm ) proved that the microspheres or nanospheres with different surface characters were obtained successfully. the clsm investigation of fitc labeled particles showed that polymeric microspheres have a core - shell structure in which the surfactants existed in the shell of particles. polymeric microspheres with different surface characters were embedded onto the surface of pla membranes via surface entrapment
激光共聚焦顯微鏡( clsm )和原子力顯微鏡( afm )觀察分析結果證實可通過上述技術獲得具有不同表面性質的微米級或納米級聚合物微球,而熒光標記技術( fitc )則證實了聚合物微球具有明顯的核殼型結構,表面穩定劑存在於微球的殼層。分享友人