agrobacterium rhizogenes 中文意思是什麼
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Transformation of glycyrrhiza inflat bat by agrobacterium rhizogenes r
發根農桿菌轉化脹果甘草的研究 -
In this study, the screening and identification of the different agrobacterium rhizogenes strains were carried out using physiological, chemical, molecular and bioassay methods, and the vigor of different strains was also compared based on their characters ; the transformation of torenia mediated by agrobacterium rhizogenes, the hairy root formation and its regeneration were importantly studied
本實驗研究了（ 1 ）發根農桿菌的生理生化、分子生物學鑒定及生物檢測和活力比較； （ 2 ）發根農桿菌介導的藍豬耳遺傳轉化和藍豬耳毛狀根的植株再生，為藍豬耳的遺傳轉化和其它分子生物學研究奠定基礎。 -
Genetic transform ation of al hagi preudoalhagi with agrobacterium rhizogenes a
4對駱駝刺的遺傳轉化 -
The transformed hairy roots were detected for agropine to make sure the transformation was successful. a conclusion was reached as follows : agrobacterium rhizogenes r1601 was an ideal strain for hairy roots transformation from trichosanthes bracteata voigt which exhibited extraordinary transformation rate ( 60 % ). od600 = 0. 7 was the optimum infection concentration for the tested transforming agent, with temperature at 25, ph at 6. 0, 20min of infection and 48h of co - culture and phytohormone at 1. 0mg / l being the most favourable hairy root - inducting pattern
通過實驗和對結果的數據分析，得出下面的結論：發根農桿菌r1601是較好的大苞栝樓發根的誘導菌株，它對預培養24h的大苞栝樓子葉外植體具有最好的轉化效率和發根誘導率（ 60 ） ，最適的菌株感染濃度od _ （ 600 ）為0 . 7 ，溫度27 、 ph值為6 . 0 、 20min的感染時間和48h的共培養，以及黑暗條件和1 . 0mg / l的激素最有利於發根誘導的環境條件。 -
Aimed at developing a set of methods efficient in inducing the hairy roots with agrobacterium rhizogenes from trichosanthes bracteata voigt to meet needs for hairy roots for bioreactor culture research and application ; and also in order to investigate the roles of explants, agrobacterium rhizogenes, affecting factors and their magnitude and the underlying mechanism in the transformation, therefore to determine the optimum explants, pre - culture time, tested strain concentration, infection time, co - culture time, affecting factors and their effects working together and to obtain the optimum transforming condition by performing orthogonal test, this project was launched. with cotyledons and stems from trichosanthes bracteata voigt plantlets as explants, agrobacterium rhizogenes r1601 and r1000 as infection agents, transformation was performed using different methods and under various circumstances
為了建立一種有效的誘導大苞栝樓發根的方法及其發根培養體系，以適合於發根生物反應器擴大培養研究和應用基礎的需要；本研究對誘導發根的外植體在發根農桿菌和環境因子等影響作用下進行發根的誘發及其可能的機理進行探討，從而找出用於發根誘導和培養的最佳外植體、預培養時間、發根農桿菌濃度、感染時間和共培養時間、誘導條件，並通過正交試驗得出優化的誘導條件。 -
Agrobacterium rhizogenes ( rhizobiacease ) is a kind of gram - negative bacterium, which contains a big ri plasmid ( 200kb ) - root inducing plasmid. there are three types of ri plasmid, mannopine type, cucumopine type and agropine type, according to the type of opine synthesized in transgenic cell
根據轉化細胞合成的冠癭堿（ opine ）的類型不同， ri質粒分為3種類型：甘露堿型（ mannopinetype ） 、黃瓜堿型（ cucumopinetype ）和農桿堿型（ agropinetype ） 。 -
Chromosome numbers were no remarkable difference with the control. analysis of isoenzyme ( peroxidase, cytochrome oxidase, esterase ) and rapd indicated that the regenerated plants from protocalli were different with the control. protoplasts were isolated from agrobacterium rhizogenes a ^ transformed cell line of medicago sativa l.
染色體檢查，同工酶（過氧化物酶、細胞色素氧化酶、酯酶）和rapd分析表明，來源於抗性系原生質體的再生植株具一定的遺傳穩定性，但與野生型相比亦發生了一些變異。 -
In order to establish the genetic transformation system of saussurea medusa maxism by agrobacterium rhizogenes, some work were done. the main results were following : 1 establishment of regeneration systems two systems of regeneration from saussurea medusa maxim were established without cold treatment. the somatic embryos were induced from callus cultured in mise for 35 days. the shoots were induced from cotyledon after cultured in misc 20 days, and from leave which were cultured in misl. the experiment showed that the carbon and glycine in the medium could help to increase the regeneration rate to 95 %
為篩選水母雪蓮代謝突變體和轉基因研究奠定了一定的基礎，陳亞瓊： m質粒介導水母雪蓮的遺傳轉化及毛狀根中決刪合成的調節p義摘要也為研究類黃酮代謝途徑的關鍵酶基因的轉化、高效表達及作用機理提供了理想的實驗體系。主要的實驗結果如下： 1水母雪蓮高頻再生體系的建立通過體細胞胚胎發生途徑和器官發生途徑，水母雪蓮（ squssureamedusamaxim ）可以在常溫下獲得再生植株。 -
The purpose of this study is to provide theoretical and technical support for torenia transgenic hairy root and other molecular researches used by torenia. the comparation and identification of agrobacterium rhizogenes strains were investigated using 3 - ketolactose test, the differential acid production assay and the motility assay
採用3 -酮乳糖產物法、差異酸生成實驗和游動性實驗鑒定6種發根農桿菌供試菌株a4 、 r1205 、 r1000 、 r1601 、 r1022和15834 。 -
Studies on factors affecting the transformation of astragalus mombranaceus bunge var. mongholicus with agrobacterium rhizogenes
質粒轉化蒙古黃芪影響因素的研究 -
This paper reports hairy roots induced by agrobacterium rhizogenes and production of regenerative plants from hairy root cultures in maize
本文利用發根農桿菌遺傳轉化玉米，通過毛狀根的誘導、毛狀根的分化培養獲得再生植株。 -
But the concentration of a4 at 40 h was higher than that of other strains. four strains ( a4, r1205, r1000, r1601 ) in this study were agrobacterium rhizogenes according to pcr assay
利用pcr方法鑒定結果表明，所有供試菌株中， a4 、 r1205 、 r1000和r1601為含ri質粒的發根農桿菌，而r1022和15834未出現陽性結果。 -
2 establishment of genetic transformation system by agrobacterium rhizogenes the different types of explants ( hypocotyls, cotyledon, leaf blade, petiole ) were infected by strain r1601 of agrobacterium rhizogenes
（ 2 0mg l ）上培養30d后經過體細胞胚胎發生途徑產生大量的叢生苗；子葉培養在誘導子葉產生叢生苗培養基（ msoc ） ms ba （ 2 0 。 -
Tissue culture and plantlet regeneration from leaf explants of a. ficoidea cv. " ruliginosa " and its genetic transformation with agrobacterium rhizogenes atcc 15834 were investigated as well as changes in the contents of sucrose, nitrogen and phosphate in liquid medium during the hairy root cultures
本文研究了紅龍草（ altemantheraficoideacv " ruliginosa " ）葉片的組織培養、發根農桿菌（ agrobacteriumrhizogenes ） atcc15834對紅龍草的遺傳轉化以及毛狀根液體培養過程中培養液某些營養成分的消耗變化。
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