ampullate 中文意思是什麼
ampullate
解釋
安瓿形的-
Construction of araneus ventricosus major ampullate gland cdna library and screening spider silk gene
文庫構建和絲蛋白基因篩選 -
Construction of araneus ventricousus ampullate gland cdna library and the finds of two new spider silk genes are the bases of supplying the background of araneus ventricousus silk genes and further researching in the spider silk proteins
大腹園蛛主壺腹腺cdna文庫的構建和兩個蛛絲蛋白新基因的克隆，為提供大腹園蛛蛛絲蛋白基因背景和進一步研究蛛絲蛋白奠定了基礎。 -
The cdna library of major ampullate glands of araneus ventricousus was prepared successfully for the first time by using puc18 vector in the world. two genes encoding spider silk protein and one gene encoding no - silk protein were cloned by means of randomly picking colony ( bird gun )
本研究在國內外首次構建了大腹園蛛（ araneusventricosus ）主壺腹腺（ majorampullategland ） cdna文庫，並以鳥槍法從中篩選出兩個蛛絲蛋白和一個非絲蛋白cdna新基因。 -
Firstly, the major ampullate glands were got from araneus ventricousus. total rna and mrna of ampullate glands were isolated and purified. double strands cdnas were synthesized in the assistance of amv - rt and e. coli dna polymerase i etc. by reverse transcription and replacement synthesis
首先剝離大腹園蛛主壺腹腺，提取總rna和分離純化mrna ，反轉錄合成cdna ，凝膠層析除去小於400bp片段，並在雙鏈cdna兩端引入ecor的銜接頭，對其進行磷酸化處理。
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