arginine-rich histone 中文意思是什麼
arginine-rich histone
解釋
含精氨酸豐富的組蛋白-
Arginine rich human histone h3 is a kind of basic nucleosome protein. in the medium of physiological condition, by electrostatic interaction, histone h3 which arginine impart to it a positive charge binds to dna which phosphate groups impart to it a negative charge. in this paper, in order to establish a new technology of transfection, the functional domains la and ii gene segments of pea were lined with histone h3 gene segment, then a recombinant fusion protein was constructed which serves as c arrier for transfer of dna via receptor - mediated endocytosis
本實驗在已成功獲得的pea功能區a ，功能區基因片段與人組蛋白h3基因片段的克隆菌株的基礎上，構建原核表達載體，將克隆質粒pmd - 18t - pea經nco ， mlu雙酶切，回收酶切產物；將克隆質粒pmd - 18t - h3經mlu ， xho雙酶切，回收酶切產物。
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