bacterial inclusion 中文意思是什麼
bacterial inclusion
解釋
細菌細胞內含物-
After washing with reagent, adopt the newest purification technology source30rpc, sds - page and densitometric scan analysis, the result show that expression level is 90 % of total bacterial proteins. after renaturation, ifnr, hgfa, hgfb, hpk5 were purified by akta purifier chromatogram instrument, sepharose fast flow, ssphacrayl series gel, selecting optimize condition. finally establish a kind of high efficient purification model of recombinant proteins produced in escherichia coli as inclusion bodies, purification product purity > 98 %
結論:總之，通過對發酵罐中重組工程菌各種培養因素的研究，建立了一種高密度、高表達發酵工藝體系，為重組蛋白的后續純化提供了大量、穩定的原料供應;通過對不同目的蛋白的色譜行為的系統研究，建立了一種高效穩定、快速簡潔、易於放大的包涵體重組蛋白分離純化體系。 -
The expressed fusion protein occupied more than 20 % of total bacterial protein. the fusion protein induced mainly existed in the insoluble inclusion body of the cell, only little parts of fusion protein expressed in the cytoplasm, excreted into periplasm and secreted into the cultural medium as soluble protein. through ultrasonic treatment at low temperature, soluble expressed fusion protein could be obtained from the supernatant of lysed cell
表達產物在細胞內外的精細定位研究表明，融合蛋白cbd - lt 27在經誘導的大腸桿菌中表達量占總菌體蛋白的20以上，融合蛋白主要以不溶性包涵體的形式存在於細胞中，少量以可溶產物的形式存在於細胞質、分泌于細胞周質間腔及培養基中。 -
The expressed proteins accumulated up to 40 % of bacterial soluble protein after iptg induction and formed inclusion bodies, which have no fibrinolytic activity
表達量都高達菌體可溶性蛋白的40 ，主要以包涵體的形式存在，沒有檢測到纖溶活性。 -
The expression protein products as insoluble inclusion bodies accounted for 15 % and 30 % of whole bacterial protein. two methods, b - per reagent only and triton - urine with sonication lysis of cells, were both access to the relatively good washing effect
通過摸索iptg誘導濃度，誘導溫度，菌體收獲時間等條件，確定了eo基因能在pgex4t bl21 （ de3 ） codonplus和pmal - p2x bl21 （ de3 ） codonplus中高效表達，表達產量分別占菌體總蛋白的15和30 ，表達的重組蛋白主要為包涵體。
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