binding antibody 中文意思是什麼
binding antibody
解釋
結合抗體-
Second, ca - np30 conjugate was made by binding ca with np30. then immunized balb / c mice with ca - np30, and evaluated its effects on protective immunity, serum specific igg antibody levels and delayed type hypersensitivity ( judged by footpad test )
將鈣納米顆粒ca與np30制備成ca皿30結合物（ ca np30 ） ，主動免疫b從b兒， j 、鼠，觀察其對， j 、鼠抗血吸蟲感染的保護作用、對血清特異性屹g抗體的影響和足墊反應。 -
The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。 -
Because the analyte molecules are recognized by forming the binding complex with antibody, a decrease in the detection limit is achieved only by an increase in the reaction yield
因為分析物分子能通過與抗體形成結合物而被發現，所以降低檢測極限只能通過增加反應產率來達到。 -
Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use
X陽性克到駛知烘扳原kbbjg ）濁對陽性克隆進行限制隴臥賜析（ uwi和hedlll ）鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率，從中j ） ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。 -
Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。 -
Methods according to theory of specific binding of antigen and antibody, at first the anti - a monoclonal antibody ( ma ) and anti - bma were labeled with the fluorescent, then fluorescent - labeled antibodies ( fla ) were bound with corresponding biological material ( such as bloodstain ) in the optimum condition, finally the abo blood type of bloodstain was determined under microscope fluorescent
方法根據抗原抗體特異性結合的原理，首先對抗a 、抗b單克隆抗體進行熒光標記，然後使熒光標記抗體與相應抗原（血痕）在最佳條件下結合，最後熒光顯微鏡鏡檢，判定血痕的血型。 -
Biological recognition with enzymes or antigen antibody binding are used to achieve high specificity
酶的生物識別或抗原抗體結合使光纖傳感器可以獲得高的特異性。 -
Because the antigen ? antibody reaction is an equilibrium binding reaction, the magnitude of the signal relies on the concentration of each reactant
因為抗原抗體反應是一個平衡結合反應，所以信號量依賴于每個反應物的濃度。 -
The binding reaction between antigen and antibody can be enhanced by optimizing chemical environments, such as acidity and ionic strength, to which the immunoreactants are exposed ( 34, 35 )
抗原和抗體間的結合反應可通過優化免疫反應物暴露的化學環境，例如酸度和離子強度，來提高優化。 -
Screening the antibody mimic peptide binding to hepatocellular carcinoma cells by phage display technique
用噬菌體表面展示技術篩選與肝癌細胞系結合的抗體模擬肽 -
One n - peptide - binding scfv clone was selected from the phage antibody library using affinity panning. the target antigen, n - peptide was biotinylated using photobiotin first
N -肽是一種新發現的神經肽，其n端與阿片肽高度同源，可與阿片肽受體相互作用。 -
( 4 ) in artificial pepsin digestive system with ph2. 0, due to the acid denaturation, the pigg was hydrolyzed wholly, in the system with ph3. 0 and 4. 0, although not all entirely pigg could be detected, but the pigg antibody activities against e. coli still existed without changing, this indicates that the f ( ab " ) 2 with antigen - binding activity still remain complete during digesting process
（ 4 ）在ph2 . 0的人工胃液消化體系中，由於酸變性pigg全部水解；在ph3 . 0和ph4 . 0的人工胃液消化體系中，盡管檢測不到所有的pigg ，但pigg仍可與e . coli特異性結合且凝集價未發生變化，表明在消化過程中具有抗原結合活性的f （ ab ） _ 2片段仍可完整地保留下來。 -
Construction of murine phage antibody library and selection of n - peptide - binding single - chain antibodies
小鼠噬菌體抗體庫的構建和n -肽結合單鏈抗體的篩選 -
Expression of a candidate human era binding protein a19 and the preparation of its polyclonal antibody
的原核表達及其兔抗體的制備 -
In addition, the display of repertoires of antibody fragments on the surface of filamentous bacteriophages offers a new way of making antibodies with predefined binding specificities
高親和的抗乙肝病毒表面抗原pres1 （ 20 - 47 ）抗體具有封閉hbv的作用。噬菌體展示技術提供了獲得結合特異表位抗體的新途徑。 -
Molecular recognition is utilized to describe the molecular binding process in a specific manner. it ' s well known that the antibody / antigen, receptoraigand and enzyme / substrate are the three molecular recognition models in animate bodies
分子識別是分子間專一性的結合過程，抗體與抗原、受體與配體、酶與底物是人們所熟悉的生物體內的分子識別的三個模型。 -
The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。 -
5. preparation of rabbit anti rat lysosomal copper binding protein polyclonal antibody
用分子量在58kd的蛋白質免疫家兔，得到多克隆抗體。 -
Fortunately, recently widely used bispecific antibody technique provided us a good model for target rca blocking for it ' s dual antigen binding activity and for the availablity of high
為了進一步探討用基因工程的方法制備抗病毒雙特異性抗體的可能性，我們又在現有的條件下構建、表達了 -
Using a mouse pup model of rotavirus infection, the scientists demonstrated " efficient binding of the vhh antibody fragments to rotavirus, " resulting in a significant reduction in rotavirus infection in cell cultures
選用輪狀病毒感染的幼鼠模型，以證實「 vhh抗體片斷能有效與輪狀病毒結合」 ，細胞培養結果顯示有輪狀病毒顯著意義減少。
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