blot up 中文意思是什麼
blot up
解釋
吸干-
2. effect of nacl treatment on the subunits expressions and activities of h + - atpase and h + - ppase from suaeda salsa northern blot analysis indicated that the transcription of v - h + - atpase subunits b, h and c were obviously up - regulated by nacl stress. the transcripts of mrna of b and h subunits in 400 mmol / l nacl - treated plants were significantly higher than those in control during 0 - 120h and the mrna levels of b, h and c subunits were also increased under 100 and 400 mmol / l nacl treatment
400mmol lnacl處理鹽地堿蓬植株0 - 120h發現鹽脅迫明顯誘導了鹽地堿鹽脅迫下鹽地堿茨液泡膜質子泵衷達分析及過量系達印mhxaw對擬甫芥耐鹽性的影響蓬液泡膜h 」 atpaseb 、 h亞基基因表達； 100 、 400mmol lnaci處理鹽地堿蓬植株，分析其葉片液泡膜h 」 -
In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。 -
The genomic dna extracted from three rubber tree strains ( pr107, rrim600, gt1 ) was digested with hinckr. the southern blot was made with the same probe as above. the result showed that all the three strains turned up only one brand ( - - 4. 8kb ). the three strains had the same inherent background
用橡膠樹pr107 、 rrim600 、 gti三個品系的基因組洲a分別經hinan酶切后，進行了southern雜交。結果表明，三個無性系都只出現一條雜交帶，位置一』致（ 4 -
I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]
從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。 -
Result a - 1bgp expression is up - regulated in hepatoma cells treated with gh by northern blot. gh increased a - 1 bgp expression after 3 hr, reaching maximal levels at 9hr
實驗結果gh 、 p ez及iys處理人肝癌bel 7402細胞后， no汕em印跡雜交分析均顯示a則與對照組相比表達增高。
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