blot test 中文意思是什麼

blot test 解釋
墨漬測驗
  • blot : n 1 墨污,墨漬,污點,污斑。2 瑕疵;恥辱,污名。3 〈古語〉塗去,抹去。vt ( tt )1 用(墨水等)弄...
  • test : n 1 檢驗,檢查;考查;測驗;考試;考驗。2 檢驗用品;試金石;【化學】試藥;(判斷的)標準。3 【化...
  1. Transgenic plants were comfirmed by molecular determination including pcr, southern blot and rt - pcr. 4. functional analysis and iron content of transgenic plant crude proteins of transgenic plant were extracted from leaves, and then used to test its antibiotic activities

    4 .轉基因植株抗菌能力加強,鐵含量得到提高提取轉基因植株葉片粗蛋白,以大腸桿菌dhsq和酵母菌ahiog為試驗靶標,側定了轉基因植株粗蛋白的抑菌活性。
  2. The result of immune blot test suggested the neutral glycoprotein was the active part of crude product and its purity was 53. 75 % rised from 29. 99 % of origin

    對分離后的兩種成分進行免疫斑點試驗,表明活性成分存在於中性糖蛋白中。純度由粗產品的29 . 99提高到53 . 75 。
  3. Ability to a - complement of the ea / ed protein was determined by the addition of onpg western blot test with rabbit to e. coli p - galactosidase pcab as first antibody was used to verify the fusion proteins

    以兔抗p半乳糖昔酶抗體做western blot以證實與gst融合表達的ea工d蛋白是p半乳糖昔酶的成分。實驗結果1
  4. 97 % identities in amino acids respectively. the e. coli strain dh5 transformed recombinant plasmid phn was induced with 0. 6 mmol / m iptg for n gene expression. the expressed product was identified by sds - page and westem - blot test, a fusion protein about 47ku as we expected was found

    將含有重組質粒phn的菌株dh5在37條件下培養,以濃度為0 . 6mmol / liptg誘導,重組質粒n基因phn融合蛋白獲得了表達:經sds - page , western - blot試驗,確定其表達的融合蛋白產物大小為預期的47ku 。
  5. In the laboratory experiment part, human peripheral blood, cultured cells and icr mice were study objects. the changes of mitotic chromosome numbers were measured by human metaphase chromosome counts and statistic analyzed used x2 - test. the changes of meiotic chromosome numbers were measured by mice one - cell zygote chromosome counts and statistic analyzed usedx2 - test. the effects of low dose ionizing radiation on the expression of topoisomerase ii were measured by immunocytochemistry, western blot and rt - pcr

    流行病學結果顯示長期小劑量輻射接觸與染色體不分離呈正相關,為進一步在細胞遺傳學和分子生物學方面研究小劑量電離輻射與染色體不分離關系及其機制,本課題第二部分以外周血、培養細胞、 icr小鼠為研究對象,用外周血染色體計數和單細胞受精卵染色體計數的方法研究小劑量輻射和拓撲異構酶復旦大學2000級博士生學位論文11a抑制劑及其二者的協同效應對有絲分裂和減數分裂染色體不分離的影響,用免疫細胞化學染色、 westernblot 、 rt pcr等方法研究了電離輻射引起拓撲異構酶a表達變化。
  6. It was transformed into competent cells of bl21 ( de3 ), then was induced to express by 0. 2mmol / l iptg at 37 for 4 hours. the recombinant protein was nearly 23. 3 % of the total products. it was proved that the recombinant protein had immune activity through western blot and elisa analysis. this provided the basis for the future preparation of mycoplasma hyopneumoniae test kit and the new vaccine against mycoplasma hyopneumoniae

    將其轉化宿主菌bl21 ( de3 )的感受態細胞,然後經過誘導條件(誘導溫度、誘導劑用量和誘導時間)的優化,結果發現在37下, iptg終濃度為0 . 2mmol l ,誘導4h進行表達,可得到了一分子量約265kda的融合蛋白,表達量可達約23 . 3 。
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