bradyrhizobium 中文意思是什麼
bradyrhizobium
解釋
短根瘤菌屬-
We conclude that the gene we studied is a new loci required for efficient nitrogen fixation and for competitive nodulation of soybeans by bradyrhizobium japonicum strain gx201
我們認為在突變體菌株gx217中tn5gusa5所插入的開放閱讀框架是一個新的與競爭結瘤有關的基因。 -
Pgxn217 was transferred into bradyrhizobium japonicum strain gx201 by triparental mating using the helper plasmid prk2073. marker exchange was achieved by selection on yma containing sm, km, gm, spc et al four kinds of antibiotics. mutants were confirmed by southern blotting and hybridization with the wild - type region and the tn5 fragment respectively
隨后，利用突變質粒pgxn217誘變慢生型大豆根瘤菌菌株gx201 ，獲得了gx201的突變體菌株gx217 ；將pgxn201的3 . 4kbecori片段與plarf3連接獲得亞克隆pgxn201cl ，然後將pgxn201cl導入突變體菌株gx217 ，構建了gx217的功能互補菌株rgx217 。 -
The 3. 4kb ecori dna fragment of the pgxn201 and the mutant ecori dna fragments of the three mutant plasmids were subcloned into the ecori sites of the clone vectors ml3 and the pgem3z - f ( + ) respectively. through dna sequencing and dna sequence analysis by comparing with the bradyrhizobium japonicum dna sequences reported, we found that there are four intact open reading frames in the 3. 4kb fragment and it was highly homologous to the bradyrhizobium japonicum dna sequence reported, and the tn5gusa5 containing in the pgxn217 was inserted in one of the four open reading frames. this open reading frame located on about 5. 5kb upstream of the nfec gene, which includes 207bp nucleotides, coding 68 putative amino acids and this is completely identical to that of the bradyrhizobium japonicum dna sequence reported
本研究首先利用轉座子tn5gusa5對重組質粒pgxn201進行誘變，獲得3 . 4kbecori片段插入了tn5gusa5的突變質粒pgxn215 、 pgxn216 、 pgxn217 。對這些突變質粒進行亞克隆及測序分析並與基因庫中已報道的慢生型大豆根瘤菌的dna序列作比較，發現突變質粒pgxn217中tn5gusa5恰好插入在一個未知功能的開放閱讀框架內。將pgxn201的3 . 4kbecori片段與m13作連接，獲得亞克隆pgxn201c ，對pgxn201c進行測序分析，發現與基因庫中已報道的慢生型大豆根瘤菌的dna序列有95的同源性。
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