cell hybridization 中文意思是什麼

cell hybridization 解釋
細胞雜交
  • cell : n 1 小室，單室；隔間，艙；〈詩〉茅舍；（單個的）蜂窩，蜂房。2 〈詩〉墓穴，墓。3 （大修道院附屬的...
  • hybridization : 混成作用
  1. That cell hybridization can be used to dissect regulatory mechanisms controlling gene expression in eukaryotic cells.

    雜交細胞能被應用於剖析真核細胞中控制基因表現的調節機理。
  2. The more accurate localization of 3galt - l mrna expression in mouse brain as studied by in situ hybridization was in good agreement with the general expression pattern seen with northern blot hybridization. in newborn mice, the dense granular layer and the purkinje cell showed a strong signal

    我們發現在小腦中1型鏈結構的變化與邵galt一1的表達變化基本一致:在新生鼠小腦中， 1型鏈結構在蒲肯野細胞和顆粒層細胞中都有表達，而在成年鼠小腦中， 1型鏈結構僅在蒲肯野細胞中表達。
  3. Half animals were perfused with paraformaldehyte following normal serum after test innnediatly, then removed the brain and fixed for 24 hours with paraformaldehyte, paraffin sections were prepared at 3um, histochemical staining for counting ce11 pro1iferating ratio and in situ hybridization for calculating integra1 scores of stem cell factor inrn positive signals

    結果如下： 1增殖細胞的觀察經72h的快眼動睡眠剝奪，實驗組大鼠海馬齒狀回區brdu與p皿a （ proliferatingcellneuclearantigen ， pma ）陽性標記細胞數顯著增加。
  4. Somatic cell hybridization is technique.

    體細胞雜交是一種技術。
  5. In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks

    方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。
  6. The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences

    用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。
  7. The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10

    重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞，兩輪空斑篩選和純化，獲得重組病毒rbmbacph - egf ，經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。
  8. In previous study, janghong have succeeded cloning 24 ests of mouse testis spermatogenic cell apoptosis - related gene by creating mouse cryptorchidism model and making use of suppression subtractive hybridization, and registered in genbank

    在前期的研究工作中，本室姜宏等通過建立小鼠隱睪模型，運用抑制消減雜交法（ suppressionsubtractivehybridization ， ssh ）克隆出24個小鼠睪丸生精細胞凋亡相關基因的est ，並在genbank中登錄。
  9. In the present experiment studies, an acute traumatic model of lateral cortical impact was employed to study expressive changes of microtubule associated protein - 2 ( map - 2 ), cyclooxygenase - 2 ( cox - 2 ), glial cell line - derived neurotrophic factor ( gdnf ), caspase - 3 mrna and protein after brain injury in rats. immunocytochemical staining, western blotting, nucleic acid in situ hybridization with an oligonucleotide probe and computer image analysis were used to detect the dynamic changes of map - 2 mrna, cox - 2 mrna, gdnf mrna, and caspase - 3 mrna in the cortex after moderate traumatic brain injury ( tbi )

    本實驗從自行設計大鼠腦損傷落體打擊器開始，先行建立了一個便於觀察和施加處理因素、控制性好、重復性好的動物模型，選用30g擊錘從25cm高處下落，沖擊應力d為355 . 09kpa ，打擊大鼠右頂部，造成中等程度的閉合性腦損傷，從病理形態學、組織超微結構觀察及微管相關蛋白- 2 （ microtubuleassociatedprotein2 ， map - 2 ） 、環氧合酶- 2 （ cyclooxygenase2 ， cox - 2 ） 、膠質源性神經營養因子（ glialcellline - derivedneutrophicfactor ， gdnf ） 、 caspase - 3基因及蛋白表達的時間性變化，詳盡系統地闡述腦損傷后各指標變化的時間規律性及表達差異可能的形成機制。
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