chloroplast genome 中文意思是什麼
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Compared with nucleic genome, the chloroplast genome is very small and easily to be manipulated genetically, and the foreign gene is site - direct integrated into the chloroplast genome through homologous recombination
與核轉化相比，葉綠體基因組小，遺傳操作簡單，外源基因是通過同源重組機制定點整合進葉綠體基因組。 -
The result demonstrated that the ctbvpl fusion gene was inserted into the c. reinhardtii chloroplast genome and after three rounds of spc selection the recombinant chloroplast dnas were the majority, and the wild - type chlorop last dnas
W七sternblot和elisa免疫雜交分析表明ctbvpi融合蛋白在衣藻葉綠體中得到表達，表達量占可溶性總蛋白量的3一4 % （ lmg可溶性總蛋白含有30一40pg的重組蛋白） 。 -
In the second part of the thesis, we described that a tobacco chloroplast expression vector, ptrvp1, containing the foot and mouth disease virus ( fmdv ) vp1 gene and the selective marker aada gene was constructed and transfered to the tobacci chloroplast genome by the biolistic method
論文第二部分主要敘述了煙草葉綠體表達載體ptrvp1的構建，並通過基因槍方法轉化煙草葉綠體基因組，獲得了3株具有壯觀黴素抗性的轉化再生植株。 -
These include the ability to target the gene of interest to site - specific areas of the chloroplast genome, high - level expression of foreign genes and increased environment security
由於植物葉綠體表達系統能夠高效表達目的基因、環境安全性高，已成為目前植物生物反應器研究的熱點之一。 -
To investigate whether it is suitable for biodegradable plastic production, we constructed the chloroplast integration and expression vector ptrv - phb, and integrated all the genes necessary for phb synthesis into tobacco chloroplast genome through gene - gun transformation. the chloroplast transformants showed phenotypically normal growth and were fertile
本研究在國內率先探討將葉綠體轉化技術引入植物生產生物可降解塑料這一領域的可行性（國外僅有日本一例） ，構建了葉綠體轉化及表達載體ptrv - phb ，通過基因槍法將phb合成相關基因導入煙草葉綠體基因組。 -
To determine that the integration of foreign genes had occurred hi the chloroplast genome at the directed site by homologous recombiantion and to evaluate the degree of homogeneity of the c. reinhardtii transformants, pcr and pcr - southern blot analysis were performed using the primers of c. reinhardtii chloroplast homologous fragment
並根據衣藻葉綠體同源片段chll基因，設計一對引物對其葉綠體dna進行的pcr和southernblot分析。結果表明外源基因已經定點整合入衣藻葉綠體基因組的chll基因中，並且經過三輪抗生素篩選所得的衣藻葉綠體轉化子中，轉基因型葉綠體基因組拷貝佔多數。 -
To determine the site - specific integration of the foreign gene into the chloroplast genome and the level of homogeneity, the three transformation lines of the second selective generation were analysized through pcr ( pcr - southern blot ) analysis using the primers of fmdv vp1 gene and homologous fragment of tobacco chloroplast, respectively
經過兩輪抗生素篩選后，採用fmdvvp1基因的一對引物和煙草葉綠體同源片段的一對引物分別對抗性植株的葉綠體dna進行的pcr和pcr - southernblot分析。
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