cleavage reaction 中文意思是什麼
cleavage reaction
解釋
斷裂反應-
With the rapid development of molecular biology, the researchers of different scientific background are provided with a good opportunity to enter the field. people can resolve some important difficult problems with all kinds of research methods and knowledge in their fields. it is chemists " tribute that they design and synthesize effective nucleic acid cleavage reagents and clarify the reaction mechanism of complexes and dna, which makes it possible to search effective remedial reagents and structural probes by molecular design
分子生物學的迅猛發展為不同科學背景的研究者涉足該領域提供了良好的機遇，人們可以利用各自領域的研究方法和知識來攻克生物學中的一些重要難題，化學家所能做的貢獻就是設計和合成一些特異識別和高效切割的核酸斷裂試劑，並闡明其作用機理，從而使通過分子設計尋找有效的治療試劑和結構探針成為可能。 -
The reaction pathways were proposed as follows : firstly, hydroxylation of aromatic ring, then ring cleavage forming dicarboxylic acid and eventually oxidation to inorganic carbon
其機理為：首先是苯環羥基化，然後環破裂生成二元羧酸，最後二元羧酸氧化降解為二氧化碳和水。 -
The detection of ribozyme gene with two cleavage sites that cleaves plrv replicase gene were present in the second part of this paper. a conserved sequence of 35s promoter of plrv was found in gene pools. two primers were designed based on the conserved sequence and bamh i and ribozyme gene. the genomic dna of potato was amplified by the primers through polymerase chain reaction ( pcr )
從許多資料中報道的plrv的35s啟動子的序列中找出一段保守序列，然後根據與核酶緊密相連的bamh和這段保守序列設計兩段引物，用這兩段引物通過pcr擴增轉基因馬鈴薯的基因組dna ，並進行檢測。 -
Constructed the fusion protein expression vector of echistatin, . and identify it by dna sequencing. the vector can express echistatin fusion protein at a high level, the fusion protein molecular weight is about 16000 da in sds - page analysis, and the fusion protein consists more than 30 % of total protein, the fusion protein has specific antigen - antibody reaction with rabbit anti - echistatin polyclonal antibody. the fusion protein include : hpk5. his + 6tag, and echistatin. echistatin can be released by cnbr cleavage
構建了echistatin融合蛋白表達載體，經dna測序鑒定正確后，溫控誘導表達，獲得了高效表達，表達產物經505一隊ge分析，分子量為16000oa ，符合預計的融合蛋白分子量，表達產物. ll 』菌體總蛋白的30 %以上， westem一blotting結果顯示，該日的蛋白能夠和echistatin多克隆抗體發生特異性抗原抗體反應，表明我們成功構建了eohistatin的高效融合表達克隆。 -
In this paper, a series of transition metal complexes were synthesized ; interaction and reaction mechanism between complexes and dna were studied. in addition, we synthesized and analyzed a sequence - specific cleavage reagent, in which oligodeoxynucleotide was recognizing group. detailed work mentioned below were carried out : 1
本文在查閱大量文獻的基礎上，以1 ， 10 -鄰菲咯啉和1 ， 10 -鄰菲咯啉- 5 ， 6二酮為配體合成了一系列過渡金屬配合物，詳細研究了它們與pbr322dna的相互作用及其機理。 -
A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses
依據apmv - 1融合蛋白（ f ）基因裂解位點的核苷酸序列與其毒力的相關規律，分別設計合成了四條寡核苷酸引物，建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應（ rt - pcr ）技術。
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