clone culture 中文意思是什麼
clone culture
解釋
克隆培養-
The results of large - scaled culture show that 48g clone crushed by tissue disintegrator in the course of initial period and its inoculating density is 0. 35g / l, a month later, the clone departed directly and its density is 1. 5g / l, the highest density of each jar could be obtained 410g, the growth speed mostly doubled per week
大規模培養結果表明在20l廣口瓶中（有效水體15l ） ， 48g克隆在起始培養時經組織搗碎機粉碎切割，切割時間10s （細胞段長度約200um ） ，並按0 . 35g l接種，一個月後不粉碎直接分瓶，分瓶密度1 . 5g l ，一個月後最大密度410g瓶，生長速度約每周翻一番。 -
An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv. the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined
將包含全基因片段的三個基因克隆以限制性內切酶消化后順次連接克隆到載體ptz18r上，構建了4個全長基因的分子克隆，分別命名為pd30343 、 pd70333 、 pd70343 、 pd70344 ，其中兩個轉移到低拷貝載體plg338上，命名為plgd30343 、 plgd70344 。 -
In the condition of suspending culture, the gametophytic clone has much higher instantaneous growth rate than stilling culture
在充氣懸浮培養條件下克隆的瞬時生長率明顯高於靜置培養。 -
This article mainly discusses the large - scaled cultural conditions of clone in gametophyte laminaria. it studies the proper temperature, light time, light intensity, the consumption of nitrogen and phosphorus, the cultural state and inoculating density on the growth of gametophytic clone, the experiment of large - scaled culture had been done under comparatively proper conditions, in the same time, we studied the growth rule of gametophytic clone in the cultural process
本文主要探討了海帶配子體克隆的大規模培養條件，對海帶配子體克隆生長的適宜溫度、光照時間、光照強度、營養鹽消耗、培養狀態以及接種密度進行了研究，並在相對適宜條件下進行了大規模培養實驗，同時，對海帶配子體克隆在培養過程中的生長規律進行了研究。 -
After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence
F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。 -
He was the member of biotech committee of northwest five province, leader of tissue culture cooperative group, deputy director of ningxia biology society. from 1988, he set up ningxia kelong bioengineering r d institute, ningxia kelong industry and commerce company, and beijing ternpc industrialization co. ltd. one after another. clone plant web
李長瀟研究員1988年創建的寧夏科隆生物工程開發研究所institute和1995年創建的寧夏科隆實業有限公司ningxia kelong co . ltd分別是民營科技企業先進單位和首家涉農高新技術企業agricultural high - new technology 。 -
Though the episode ii script made no reference of any mandalorian heritage, the expanded universe turned him into a mandalorian everyman, using jango to tie the enigmatic culture into the newly revealed reality of the clone wars
他咨詢盧卡斯授權部的資料庫管理員利蘭?契，新編出與過去大相徑庭的曼達洛故事線，並為詹高?費特寫了一篇背景故事。
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