cloned line 中文意思是什麼
cloned line
解釋
克隆株-
Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。 -
Methods : the rearranged gene fragment coding tcr y v region of the jurkat cell line was obtained by rt - pcr technique the pcr product was cloned into the eukaryocytic expressive vector pcdnas to construct pcdna3 / tcr y. after confirmed by sequncing. pcdnas / tcr y plasmids were amplified in bacteria extracted by alkaline lysismethod
方法：本文採用rt ? ? pcr的方法擴增jurkatt淋巴瘤細胞特異性重排的tcr可變區基因片段，克隆到真表達載體pcdna _ 3中，經序列測定無誤后，堿裂解法大量提取質粒，制備dna疫苗。 -
[ methods ] by using the overall rna of our previously cultured human melanoma cell line ( a375 ), full length fasl gene is detected by rt - pcr. using the cdna as template, . the extracellular domain of fasl ( fasl - ecd, 127 - 278aa ) is amplified by pcr. the pcr products are directly cloned into t vector pmd - 18t
L方法採用新鮮人黑色素瘤細胞（ a375 ） ，抽提該細胞的總rna ，進行rt一pcr反應分析a375內fasl全長編碼基因的轉錄表達，以a375細胞cdna為模板，用pcr產物直接克隆法擴增人fasl一ecd （人fasl胞外區）的編碼基因，即127一278位氨基酸殘基，而後將pcr產物直接克隆于pmd一1st載體中，獲得重組質粒pmd一t - fasl一ecd ，進行dna測序。 -
Meanwhile, the cloning and sequencing of which was also performed. and the main results were as follows : ( 1 ) only two obvious polymorphism bands were obtained in the fertile line from selective pcr amplification with 64 pairs e + 3 / m + rimer combinations, and which were named as mf - 14 and mf - 24, respectively. moreover the polymorphism band mf - 14 was cloned successfully
結果如下： （ 1 ）本實驗分別以可育系和不育系基因組dna混合池為模板，選用64對e + 3 m3引物組合進行選擴篩選，分析得到了兩條可育系所特有的差異條帶mf - 14和mf - 24 。
分享友人