cloned strain 中文意思是什麼
cloned strain
解釋
克隆株-
Envelope gene gp85 of imc10200 subgroup j avian leukosis virus was cloned and expressed in the present study. the sequence encoding the gp85 domain of imc 10200 alv - j was amplified from pgem - imc2. 2 vector, which contains env gene of alv - j imc 10200 strain, and cloned into transfer vector pfast bacl
為深入探討alv - j的亞群特性，本研究利用alv - jgp85基因兩側的序列片段為引，物從正常spf蛋雞、商品肉雞和df1細胞基因組中完整地擴增了內源性類alv - jgp85基因。 -
Used as 1. 1094 strain of bacillus subtilis as researched material, the structural genes of biotin operon ( bio operon ) were cloned, and its sequence were engineered
本研究以枯草桿菌asl . 1094菌株為研究材料，克隆了生物素操縱子基因，並對基因序列進行改造。 -
Marek " s disease virus ( mdv ) phosphoprotein 24 ( pp24 ) gene was amplified from md11 strain by polymerase chain reaction ( pcr ). then we cloned it into the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector
本研究將型mdvmd11株的pp24基因的完整orf克隆入原核表達載體pgex - 6p - 1中，重組質粒pgex - pp24轉化bl21宿主菌后，經iptg誘導表達。 -
For the first time a 1539bp squalene synthase ( asqs ) cdna was cloned from a high - yield artemisia annua strain 001 by race strategy
用race方法首次從青蒿中克隆了一個1539bp全長鯊烯合酶cdna 。 -
The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。 -
Rt - pcr was used to amplify the cdna of the genome. these cdna fragments were cloned into the plasmid pucm - t. the result indicated that the seguence of the genome was obtained. the genome of aev - nh937 composed of 7055 nucleotides, potentially encodes a polyprotein of 2134 amino acids. the genome of aev - nh937 has 94. 3 % nucleotides identity with the calnek vaccine strain of aev
把它們分段克隆在pucm - t載體上，經序列分析，獲得了aev - nh937毒株的基因組全序列及推導的氨基酸序列，基因組全長為7055個核苷酸，與calnek疫苗株具有94 . 3的同源性，編碼一個含2134個氨基酸的多聚蛋白。 -
The type ii pha biosynthesis genes cloned from b. caryophylli ys13 using the described pcr method demonstrated that pha biosynthesis in burkholderia strain has an additional pathway to the normally type i pathway
對bcaryophylliys的分子生物學研究發現，它擁有類似於假單胞菌的型pha合酶體系。 -
It was then cloned to the secreted vector - ppic9k and recombined successfully into the chromosome of pichia pastoris host strain - gsl 15 by electroporation
通過電轉化作用該基因片段被成功地整合至酵母cs115的染色體上，經過甲醇誘導之後，該基因得到了分泌表達。 -
Was multiplied and the tk gene was cloned. the cloned tk gene was retrieved by proper restrictive hemodynamics. the retrieved tk gene was labeled by digoxin according to the kit of labeling and detection of digoxin. then, the specificity and sensitivity of tk gene probe were detected with dot blot hybridization. the sequence of tk gene of nm98a strain was analysed. the result of the analysis of tk gene ' s sequence confirmed that autoploidy between tk gene of nm - 98a strain and issued strain was 99. 7 %
本研究中首次對iltv - nm98a株的tk基因進行了克隆和序列分析，結果表明： iltv - nm98a株tk基因的核苷酸序列與已發表的iltvtk基因的核苷酸序列具有高度的同源性，兩者之間僅相差4個核苷酸，同源性高達99 . 7 ，從而證實了iltvtk基因是高度保守的，為iltvtk基因核酸探針的制備提供了有力的依據。 -
Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ), strain bl21 ( de3 ), to study their expression in prokaryotic cell. the gene was expressed under t7 promoter with a fusion protein partner of thx. tag and a 6x his. tag at its n - terminal. having been induced by iptg for 4 hours, the recombinant enzyme was examined in the cytoplasm by sds - page analysis
將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 （ a ） +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位於類凝血酶基因上游，並在其n端帶6xhistag標簽以利於表達產物的分離純化，經熱激轉化至宿主菌bl21 （ de3 ）中， iptg誘導斗小時后收獲菌體。 -
This thesis studied on bacillus thuringiensis strain bt886 which was separated and selected by researchers of our laboratory. according to the observation of crystal shape and the bioassay of motschulsky and fairmaire, bacillus thuringiensis strain bt886 was identified as cry3 type, and the conclusion was assured by the further study on molecular level. the 1956 base pairs full lengrh homological cry3aa gene which was toxic to motschulsky was cloned and sequenced
以該菌株為材料，克隆出了對光肩星天牛（ anoplophralabripennis （ motsch . ） ）具有毒殺作用的cry3aa同源基因，並且對該基因進行了人工改造、人工合成、大腸桿菌表達、生物活性測定、雙元表達載體的構建以及對楊樹的轉化等一系列研究，主要結果如下： （ 1 ）顯微觀察該菌株所形成的伴孢晶體為方形。 -
2. cloning and functional expression of a heterogenous gene from a. nidulans in e. coli strain a. nidulans chromosome dna was used as template to amplfy gene qutb by pcr, the dna sequencing showed that the cloned gene was in agreement with the reported sequence
異源奎尼酸脫氫酶（ qdhase ）在大腸桿菌中功能性表達以構巢麴黴菌（ a . nidulans ）基因組dna為模板，用pcr方法擴增得到了qutb基因，序列測定與文獻報道一致。 -
The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing
豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。 -
With the procedures, the overall recovery of enzymatic activity reached 20 % and the specific activity for substrate hydantoin was about 4 u / mg protein. the purification factor was about 4. 7 folds with the purity about more than 95 % as estimated by sds - page analysis. d - hydantoinase gene from strain ss - ori was cloned to five different vectors to be five recombinant plasmids and in turn to transfer into five different e. coli strains, respectively
對其中產酶活性最高的一工程菌pexsec一hdt /雲coljblz ] （ de3 ）海因酶的表達條件進行了研究，目的蛋白的表達量約占總菌蛋白的20 % ，其酶活力為0 . 92u / ml ，約是原始菌株的d一海因酶表觀活性的4 . 6倍。 -
Successfully cloned and constructed infectious full - length cdna of attenuated lapinized csfv chinese - strain ( derived from spleen ) could make us get pure rna virus genome of csfv c - strain, and further study and utilize mutation, deletion, insertion and substitution of csfv gene on dna molecular level
中國豬瘟兔化弱毒（脾淋毒）全長感染性cdna的克隆和構建，可以使我們得到純粹的csfvc -株rna病毒基因組，在dna水平上研究和利用csfv的基因突變、缺失、插入和替換。 -
The expression conditions of e2 gene in p. pastoris were optimized, the results indicated that the peak obtained after 72 hours ; pattern of inhibition / induction could improve expression level ; the best ph value were between 7. 5 and 8. 0 and the optimized methanol - induced concentration was 2 % - 3 % the e2 genes of the prevalent strain ( guangxi yulin strain ) and c strain derived from rabbit spleen tissue were amplified and cloned into e. coli the expression vector pproex - htb respectively, the recombinant plasmids pproex - gxyl and pproex - c were obtained and then were transformed into the dh5a e. coli competent bacteria respectively, the recombinant bacteria could express the major antigen region of e2 gene, the expression yields amount to 35 % and 38 % repectively
豬瘟病毒ez基因的原核表達： pcr擴增出當前豬瘟流行野毒株，中國豬瘟兔化弱毒（ c株）兔脾組織毒ez基因的主要抗原區，將其克隆到原核大腸桿菌表達載體pproex htb中誘導表達，經sds page檢測表明，重組質粒能表達ez基因主要區蛋白， westernblot檢測表明，誘導表達蛋白與豬瘟陽性血清發生特異性反應，表達量為35和38 ，可用於基因工程診斷抗原。 -
Sequence analysis indicated that the opening reading frame of ns1 gene is 1056nt in length, encoding 352 amino acids, identical to the sequence of strain sa 14 - 14 - 2 deposited in genbank. ns1 gene in the pmd18 - t - ns1 was cut by bamhi and hindiii, was cloned into the expression plasmid pet - 30b ( + ) treated with the same enzymes, resulting in a recombinant plasmid pet - 30b - ns1. the pet - 30b - ns1 was identified by pcr, restriction digestion, and sequencing
通過序列分析結果表明， jev - ns1基因編碼區核苷酸序列長度為1056bp ，編碼352個氨基酸，序列分析和比較表明本實驗克隆到的ns1蛋白基因與genbank中收錄的疫苗株sa14 - 14 - 2的核苷酸序列一致，表明盡管該疫苗株在我國應用多年，但ns1基因並未發生變異，提示該疫苗株遺傳性狀比較穩定，是一株優良的疫苗株。 -
A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally
進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。 -
Acetylornithine deacetylase is the key enzyme of producting l - methionine. we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase. in order to get high expression deacetylase strain, we obtain the gene by pcr arge gene. the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr. then taking the nucleotide sequencing compared with the sequence at blast of u. s. a. we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g
為了獲得高效表達的脫乙酰鳥氨酸酶工程菌株，在工程菌技術改造及其固定化研究做了進一步的研究和探討。我們採用基因工程技術，通過pcr技術擴增出了酰化酶關鍵酶基因?脫乙酰鳥氨酸酶基因arge ，將其克隆到puc19載體中，經酶切鑒定、 pcr鑒定篩選出重組陽性質粒，並測序鑒定，通過美國blast程序進行了基因數據庫相似性比較分析。 -
Therefore, the 4. 4kb fragment from pbl - 2 was ligated to a shuttle vector pht304 with bt replicon and transformed into acrystalliferous mutant 4d10 ( serotype hm, ) and resulted in a cloned strain bl - 3. this strain could express only 130kda protein
它不僅對蘇雲金芽胞桿菌工程菌的構建具有現實意義，而且對其它基因高表達研究將產生積極影響，具有一定的理論意義和潛在的應用價值
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