cluster insertion 中文意思是什麼
cluster insertion
解釋
成群插入-
In this study, the avermectin - producing strain streptomyces avermitilis was studied and the avermectin biosynthesis gene cluster in the genomic dna of streptomyces avermitilis s - 2 was altered by the method of gene engineering. insertion inactivation of aved gene in the cluster by introducing apramycin resistance gene into aved gene resulted in the disappearance of " a " components of avermectins. when avec gene was inactivated by the same way, four " 1 " components were lost and only " 2 " components, the potential precursor of ivermectin, were accumulated
將該基因簇中的aved基因通過插入外源的安普黴素抗性基因片段使其失活,導致發酵產物中4個a組分(不需要的組分)的消失;將基因簇中的avec基因通過同樣手段,使其失活,導致發酵產物中4個「 1 」組分的消失,而主要積累「 2 」組分(進一步改造可成為伊維菌素的前體b _ 2組分) 。
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