cpti 中文意思是什麼

cpti 解釋
三大協會及美國鋼管進口委員會
  1. 4. the construction of middle - clone vector and expression vector the puc - cp and pgem - 7z plasmid were digested by kpnl and bamhi, and collected the digested cpti fragment and the pgem - 7z, then ligated by t4 dna ligase and formed the pgem - cp

    中間載體及表達載體的構建將puc - cp質粒和pgem ? 7z質粒，用kpni和bamhi酶切，分別回收cpti片斷和酶切后的載體片段，用t _ 4連接酶連接構建成中間載體pgem - cp 。
  2. Study on expression characteristics of cpti gene in transgenic plants of rice

    基因在轉基因水稻中的表達特性研究
  3. Genetic characters of transgenic upland cotton cultivars carrying triple genes bt cpti gna

    轉三價抗蟲基因棉花遺傳特性研究
  4. Temporary fluctuation of resistance of the transgenic cotton harboring bt cpti double - gene to helicoverpa armigera

    雙價基因抗蟲棉花的抗蟲性表現特徵
  5. Inheritance analysis of resistance of the transgenic cotton harboring bt cpti double gene to helicoverpa armigera

    雙價基因抗蟲棉棉鈴蟲抗性的遺傳分析
  6. Genetic stability of transgenic bt cpti cotton and its resistance to helicoverpa armigera in later developmental stage

    雙價基因抗蟲棉抗棉鈴蟲性的時空表達
  7. Resistance of transgenic cottons expressing bt and cpti insecticidal protein genes to helicoverpa armigera

    雙價抗蟲棉的遺傳穩定性及生育後期對棉鈴蟲抗性表現
  8. Effects of cpti - bt transgenic cotton and bt fransgenic cotton on survival, growth and nutrition utilization of helicoverpa armigera h 252 ; bner

    不同轉基因抗蟲棉對棉鈴蟲抗蟲性的時空動態
  9. A great deal of modified cpti transgenic rice was obtained in successfully by our laboratory which represented on a good insect - resistance

    本實驗室成功地獲得了大量轉修飾cpti基因的水稻，表現了良好的抗蟲性。
  10. It was testified that the antibody can special immunological recognition the protein gst - cpti and cptl by indirect enzyme linked immunosorbent assay ( elisa ). the coefficient of correlation is significant and the potency is more than 1 : 800

    經間接elisa法檢測，抗體能與gst - cpti 、 cpti蛋白特異性結合，其相關系數達到顯著水平，效價1 800 。
  11. The cowpea trypsin inhibitor ( cptt ) gene is testified as a broad spectrum insect - resistant gene at present and its application in insect - resistant botanic transgenic engineering only after s / gene. the cpti transgenic plant developed rapidly for it ' s broad spectrum insect - resistant character and the target insects are uneasy tolerance to it

    豇豆胰蛋白酶抑制劑（ cpti ）基因是目前在植物抗蟲基因工程中應用僅次於bt基因的廣譜性抗蟲基因。鑒於它抗害蟲的廣譜性和靶標昆蟲不易對其產生耐受性的優點，轉cpti基因植物得到了迅速的發展。
  12. With the base of related research on nucleic acid immunization, the technology was used to develop th e research and application of cpti transgenic plant. a new antibody preparation method by nucleic acid immunization to assay the expression of the transgenic gene was explored and compared with the traditional one which immune animal by protein

    在國內外大量核酸免疫的研究基礎上，本研究首次將核酸免疫技術應用於轉基因植物檢測研究中，探討一種核酸免疫法制備抗cpti抗體來檢測基因表達的方法，並與傳統的蛋白免疫方式制備抗體進行比較。
  13. By means of plant genetic engineering, foreign insects resistance gene can be transferred into plant cell. we cloned the cpti gene and transferred it into mustard by agrobacterium - mtdi & ted transformation method. and obtained the transgenic mustard plants. the main results are as follows : 1. isolation of cpti gene total rna was isolated from cowpea seedss cotyledons and leaves. the cpti gene fragment was amplified by rt - pcr using sequences of its two sides as primers

    本實驗是利用植物基因工程獲得抗蟲的轉基因芥菜植株，結果如下： 1豇豆胰蛋白酶抑制劑基因的分離分別提取豇豆種子、子葉及葉片的總rna ，逆轉錄成cdna 。以豇豆胰蛋白酶抑制劑基因兩端的序列為引物，用rt - pcr的熱啟動方法從上述cdna中擴增出目的基因片斷。
  14. By immune the animal with the protein, the antibody which is anti - gst - cpti fusion protein was prepared. lt was testified that the antibody can special immunological recognition the protein gst - cpti, gstand cpti by indirect elisa. the coefficient of correlation is significant and the potency is more than 1 : 8000

    對其進行細菌表達后，利用glutathionesepharose4b親和柱純化獲得gst - cpti融合蛋白，免疫動物后制備了相應的抗gst - cpti融合蛋白抗體。
  15. In this article, the highly - efficient plant vectors with bivalent genes have been constructed by combining the gene of vhb with bt gene and cfmcryla - cpti gene respectively, studying the influence over the plants metabolic level and growth. we manage to reach the goal of increasing the yield of transgenic plants and cultivate new plant varities with pest - resistance by applying transgenic approaches, and eventually in the hope of cultivating new varities of crops with hoth highly - steady - yield and pest - resistance

    本文嘗試將透明顫菌血紅蛋白（ vhb ）基因優化、人工合成並且分別和gfmcryia （ bt ）基因、融合殺蟲基因（ bt - cpti ）構建成雙價基因植物高效表達載體，導入煙草，研究該基因對植物代謝水平和植物生長的影響，嘗試通過基因工程育種技術增加轉基因作物產量的同時，雙可使其具有抗蟲性，以期望培育出高產、穩產又具有抗蟲效果的新品種。
  16. T. identification of charactrization of transgenic mustard plants the putative transformant regeneration plants were assayed by pcr and pcr - southern blot analysis. both analysis the target bands were observed. so the integration of the cpti gene into mustard genome dna was confirmed. the result of insect - resistance showed that the transgenic plants are more resistant than non - transgenic plants

    轉基因芥菜植株的鑒定取再生苗的葉片提取dna ，進行pcr擴增和pcrsouthernblot分析，轉化再生植株大部分呈陽性，而非轉化的再生植株均為陰性，證明cpt基因已存在於芥菜基因組中。
  17. By the same method, the expression vector pbi121 - cp was constructed from pgem - cp and pbi121 with xbal and saci digestion. after that, the pbi121 - cp was transferred into agrobacterium lba4404 strain by freeze - thaw method. the pcr amplification indicated the lba4404 strain containning cpti gene. the lba4404 strain was used in genie transformation of mustard

    經酶切和pcr擴增驗證后，以xbai和saci雙酶切pgem ? cp和pbi121 ，將切下的cpti片段和pbi121載體片段連接構建成表達載體pbi121 - cp ，用凍融法導入農桿菌lba4404 ，提取質粒，經pcr擴增檢測，用於芥菜的遺傳轉化。
  18. Now the biological and molecular assays are the major methods to assay cpti transgenic plant. there is no better method on assaying the expression of the transgene. the reason would be that the outcome of expression by cpti is a little peptide and its molecular weight is very small. its immu - nogenicity would be insufficiency so that it can not induce strong immu - nological reaction and can not produce special antibody of high potency

    目前對轉cpti基因植物的檢測主要集中在生物測定和分子檢測，尚無較好的基因表達產物的檢測方法，其主要原因在於cpti基因的表達產物是小分子多肽，分子量小，免疫原性不夠，直接免疫不足以產生較強的免疫反應，不能產生效價高的特異性抗體。
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