degenerate sequence 中文意思是什麼

degenerate sequence 解釋
簡並序列
  • degenerate : vi 1 腐化,墮落,頹廢。2 衰敗;【生物學】退化 (to);【生理】 變質。adj n 1 腐化的,墮落的,頹廢...
  • sequence : n 1 繼續;接續;連續。2 順序;程序;次第;關系;關聯。3 後果;結果;接著發生的事;後事;後文。4 ...
  1. Using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, tow different fragments of sox gene, essox3 and essox22 were amplified from female and male eriocheir sinensis, the sequence results indicated that essox3 and essox22 shown high homology to human sox genes, and the identities to human sox genes in dna sequence and amino acid sequence are 84 % 、 85 % and 97 % 、 81 %, respectively. it might be concluded that sox gene was highly conservative in phylogenesis

    二、研究論文1 、參照人sry基因hmg - box保守區的序列,設計一對兼并引物, pcr擴增了中華絨螯蟹的sox基因,並對擴增產物進行了克隆和測序。結果在雌雄個體中篩選出兩個不同的sox基因essox3和essox22 ,其dna序列和編碼的氨基酸序列與人相應sox基因的相似性分別為84 % 、 85 %和97 % 、 81 % ,顯示該基因在進化上具高度的保守性。
  2. Using rt - pcr with two degenerate primes designed from conserved amino acids of cdpk kinase and autoinhibitory domains, three 618 bp cdna fragments ( 618 - 1. 618 - 2 and 618 - 3 ) were also isolated from broad bean leaves, and a partial cdna vjcpk2 lacking 5 " end was isolated from broad bean leaves with two gene - specific primers designed according to the 618 - 1 sequence

    用rt - pcr技術,以cdpks激酶區和連接區的保守氨基酸設計的一對簡並引物,從蠶豆葉片中還擴增到了3個618bp ( 618 - 1 、 618 - 2和618 - 3 )的cdna片段;用race技術,以618 - 1序列設計的一對基因特異性引物,克隆到了還缺少5 』末端的cdna片段vfcpk2 。
  3. These results revealed that halocin c8 is cytocidal and its primary target might be located in the cell wall of the sensitive cells. two degenerate oligoxynucleotide primers ( c8 - 2, c8 - 4 ) were designed according to the n - terminal amino acid sequence of halocin c8

    根據嗜鹽菌素cs的n端氨基酸序列設計了一對簡並引物( cs一2 , cs礴) ,利用這對引物從as7002的總dna中擴增出halcs的部分序列。
  4. Part two : studies ; l. the sox gene of dinodon refozonatum was amplified by using a pair of primers which can amplify the conservative motif ( hmg - box ) of human sry gene. the amplification band was observed in both male and female dinodon refozonatum, whose length was consistent with that of human sry gene, which about 220bp. the result of sscp analysis showed that there were many differences in the sox gene sequence between dinodon refozonatum and human, and there was a few differences between male and female dinodon refozonatum. 2. using a pair of degenerate primers based on the conservative region, hmg - box, of human sry gene, six different fragments were amplified from either female or male dinodon refozonatum, then cloned by using pmd18 - t vector and sequenced

    結果顯示: ( 1 )赤鏈蛇基因的擴增片段與人sry基因擴增片斷大小相同,為220bp左右; ( 2 )雌、雄赤鏈蛇sox基因的擴增片段大小雖然與人的相同,但其單鏈遷移率與人的有較大差異,而且雌雄個體間有明顯差異,預示該基因的dna序列在雌雄個體中可能有差異; 2 、參照人sry基因hmg - box保守區的序列,又設計一對兼并引物,擴增了赤鏈蛇的sox基因,並對擴增產物進行了克隆和測序。
  5. Using the method of parabolic regularization, we first study the existence of the optimal control of nondegenerate systems governed by where and jn is mollifying sequence in r. by de giorgi - nash ' s technique, we obtain some uniformly estimates of solutions of regularizing prob - lem, from compactness, we prove the existence of the optimal control of degenerate system by a approximate approach

    利用拋物正則化方法,我們首先研究了非退化系統最優控制的存在性,其中而j _ n是r上的軟化子。利用degiorgi - nash技巧,對正則化問題的解可以得到一些一致估計,利用緊性,通過一個逼近過程我們得到了退化系統最優控制的存在性。
  6. With h. trueperi genomic dna and degenerate primers, a 560 bp pcr fragment was obtained and labeled as a probe. after h. trueperi genomic dna was digested with different endonucleases, southern blot result showed a 2. 6 kb positive fragment digested by ecori and ipcr was carried out to obtain the flanking sequence

    將擴增片段用地高辛標記成探針,與用不同限制性內切酶完全酶切的h . trueperi總dna片段作southem雜交,結果顯示在ecori酶切片段的2 . 6kb處有陽性信號。
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