dna plasmid 中文意思是什麼
dna plasmid
解釋
dna質粒-
The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest. then the recombinant plasmid was identified by endonuclease analysis, pcr ampliation and dna sequencing. the report showed that the recombinant plasmid had right open reading frame
重組質粒經酶切鑒定， pcr鑒定和測序，結果證實豬肺炎支原體黏附因子p97基因的抗原決定簇r1區定向插入了質粒pet - 32a （ + ） ，且閱讀框架正確。 -
Protective immunity to schistosoma japonicum elicited by co - immunization of cathepsin b dna vaccine with eukaryotic plasmid encoding il
4真核表達質粒聯合免疫誘導小鼠保護性的研究 -
In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli
根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。 -
Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。 -
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
Sedegah m, hedstrom rc, hobart p, et al. protection against malaria by immunization with plasmid dna encoding circumsporozoite protein [ j ]. proc natl acad sci usa, 1994, 91 ( 21 ) : 9866
劉彥文，余新炳，徐勁等.惡性瘧原蟲海南株環子孢子蛋白基因的克隆與表達[ j ] .中國人獸共患病雜志， 2000 ， 16 （ 1 ） : 8 -
4. the construction of middle - clone vector and expression vector the puc - cp and pgem - 7z plasmid were digested by kpnl and bamhi, and collected the digested cpti fragment and the pgem - 7z, then ligated by t4 dna ligase and formed the pgem - cp
中間載體及表達載體的構建將puc - cp質粒和pgem ? 7z質粒，用kpni和bamhi酶切，分別回收cpti片斷和酶切后的載體片段，用t _ 4連接酶連接構建成中間載體pgem - cp 。 -
At first. then eight a - amylase gene fragments were cloned with the genomic dnas as templates by routine pcr. following that, these gene fragments and plasmid vectors, pbluescript ii ks + and puc18, were cut by bamh i and kpn i. the prepared insert dna and vector dna were linked by t4 dna ligase
利用vectornti6 . 0軟體，對所克隆的序列用相鄰接點法（ neighborjoining州j ） method ）進行多序列比對，分析其同源性，並構建基因進化樹。 -
The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme, were linked by means of t4 dna ligase and transformed into e. coli jm109 permissive cells, yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid
將重組質粒pugedna與轉移載體pfastbacldna用bamhi和ecori雙酶切處理， t _ 4dna連接酶連接，用連接產物轉化大腸桿菌jm109感受態細胞，得到重組轉移載體質粒pfastbac - gedna 。 -
An improved alkaline lysis adaptive to extracting plasmid dna of bacillus
提取的改良堿裂解法 -
In this sense, ida plays dual effects, tridentate chelating to cu ( 2 ) and bridge between two cu ( 1 ) andcu ( 2 ). 3 metal complexes were selected as the appropriate for the study of cleavage plasmid pbr322dna by gel electrophoresis technique. the results showed ni and mn complexes could cleave effect - ively dna in the presence of h2o2 at physiological ph and temperature, whereas individual zn complex could cleave effectively dna
通過電泳實驗研究了一系列金屬配合物與pbr322dna的作用，發現在tris - hcl緩沖溶液中，生理條件下，鎳、錳配合物在共反應物h _ 2o _ 2存在下能夠很好的斷裂dna ，而zn配合物單獨作用就能夠使dna由ccc型轉化為oc和linear型。 -
The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak. 8. bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent
將克隆到的hbmp基因通過適當的酶切插入到轉移載體質粒pbac - pak8的多克隆位點中，獲得重組轉移載體質粒pbacpak - hbmp 。 -
To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively
鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。 -
Then the plasmid was extracted from them and determined by dna calculator. the protoplast that contain growth hormone releasing factor injected into rabbit muscle and mouse muscle after it were treated by 1 % glutaral, pay it to electric stimulation in muscle of injection site and extract omni - rna from injection site of rabbit muscle, expression of grf were detected by reverse transcription and pcr ; ratio of expression of grf were detected by elisa. extract dna form injection site of mouse muscle to research time of expression
本實驗是將含grf重組質粒的jm109菌株大量培養，用堿裂變法提取質粒，用dnacaculator定量；制備含grf的原生質體，經1的戊二醛處理后注射於家兔肌肉，在注射部位給予電刺激，提取總rna ，用rt - pcr檢測grf在肌肉中的表達；用elisa法定性檢測grf在肌肉中蛋白質水平的表達；將該原生質體注射于小鼠肌肉中，定期提取dna ，初步探討原生質介導的外源性grf在小鼠肌肉中的表達時間。 -
After the plasmid was assayed for dna sequence, it was transformed into gs115 by electroporation
利用限制性內切酶分析、 a序列測定在a水平對質粒進行鑒定。 -
By recombinant dna techniques, the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb. the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e. coli dh5a and induced with iptg. sds - page analysis showed an induced expression product band about 72ku, which correspond to the sizes of vp2, reported in the literature
利用dna重組技術，將其結構蛋白vp2基因亞克隆至原核表達載體pproex - htb ， iptg誘導后成功表達出與預期大小相符的約72ku的融合蛋白，光密度掃描對表達產物進行初步定量，表明表達產物約占菌體總蛋白的14 。 -
In two of these isolates with typical class 1 integron, it was showed that the integrons were both harbored in plasmids after intll southenbloting both on their genome dna and plasmid dna
1株福氏志賀菌y變種攜帶的基因盒類型與其中一株宋內志賀菌相同，為護漢17和aad減萬。 -
According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene
因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。 -
It was showed under the laser scanning confocal microscopy that the transfected plasmid dna transcripted by rna polymerase and its transcripts were both localized in the interior and periphery of nucleoli
由此我們認為rna聚合酶的轉錄就是發生在這一區域里，而並非是人們傳統認識中的核質中。 -
The 496 bp fragment of the orf of p22 gene and a 561 bp fragment were amplified from the genomic dna of zs strains of toxoplasma gondii. both 496 bp and 561 bp fragments were successfully cloned into the plasmid pthiohisa, b, c and pbudce 4. 1 respectively. 2
從弓形蟲zs株基因組中擴增出p22編碼基因的一長496bp ，另一長561bp的片段，並成功構建含p22編碼基因的原核質粒重組體pthiohisa ， b ， c / p22 ，及真核重組表達質粒pbudce4 . 1 / p22 。
分享友人