dna sequencing 中文意思是什麼

dna sequencing 解釋
dna測序
  • dna : 1. deoxyribonucleic acid 【生物化學】去[脫]氧核糖核酸。2. deoxypentose-nucleic acid 去[脫]氧戊糖核酸。
  • sequencing : 測序，序列測定
  1. The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest. then the recombinant plasmid was identified by endonuclease analysis, pcr ampliation and dna sequencing. the report showed that the recombinant plasmid had right open reading frame

    重組質粒經酶切鑒定， pcr鑒定和測序，結果證實豬肺炎支原體黏附因子p97基因的抗原決定簇r1區定向插入了質粒pet - 32a （ + ） ，且閱讀框架正確。
  2. To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively

    鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。
  3. Wheeler ' s team used dna sequencing and computer simulations to reconstruct the evolutionary tree of venomous fish ? all the way back to their common ancestor

    威勒的團隊使用dna排序和計算機模擬的方法重構了有毒魚類的遺傳樹，一直追溯到它們的共同祖先
  4. Objective : to elucidate the characterization of katg, inha, ahpc, kasa, and oxyr gene mutations in isoniazid - resistant clinical isolates of mycobacterium tuberculosis, and discuss the value of judging the susceptibility of mycobacterium tuberculosis strains to isoniazid by dna sequencing

    目的闡明結核分枝桿菌耐異煙肼臨床分離株katg基因、 inha基因、 ahpc基因、 kasa基因及oxyr基因突變特點，探討是否可能通過dna序列分析來判斷結核分枝桿菌對異煙肼的敏感性。
  5. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  6. 2. cloning and functional expression of a heterogenous gene from a. nidulans in e. coli strain a. nidulans chromosome dna was used as template to amplfy gene qutb by pcr, the dna sequencing showed that the cloned gene was in agreement with the reported sequence

    異源奎尼酸脫氫酶（ qdhase ）在大腸桿菌中功能性表達以構巢麴黴菌（ a . nidulans ）基因組dna為模板，用pcr方法擴增得到了qutb基因，序列測定與文獻報道一致。
  7. Supply of concentrate haemodialysis solution with bicarbonate powder buffer to the hospital authority and the department of health as a 24 - month contract from date of acceptance design, supply, delivery, installation, commissioning, maintenance of hardware, software and related services for the implementation of the automated tag and information display system for the immigration department on or before december 2006 supply of 320 000 kg. of polyelectrolyte type ii to the drainage services department as a 36 - month contract from date of acceptance provision of dental laboratory work for the department of health as a 24 - month contract from date of acceptance supply, installation and commissioning of a ground reception system for meteorological data from multi - functional transport satellite for the hong kong observatory from date of acceptance to fulfillment of contractual obligations supply and installation of 1 set of automated dna sequencing system to the department of health from date of acceptance to fulfillment of contractual obligations

    承投為醫院管理局和?生署供應高濃度血液滲析液連炭酸氫鹽緩沖粉劑，合約由發出接納書日期開始，為期24個月為入境事務處於2006年12月或之前推行自動化籌號及資訊顯示系統供應硬體和軟體，包括設計、送貨、安裝、試機、保養及有關服務為渠務署供應320000公斤高分子電解質（第ii類） ，合約由發出接納書日期開始，為期36個月為?生署提供牙科製品服務，合約由發出接納書日期開始，為期24個月為香港天文臺供應一套多用途輸送衛星氣象數據地面接收系統，包括安裝及試機服務，由發出接納書當日至履行合約訂明的責任為止為?生署供應和安裝一套核酸序列自動測定系統，由發出接納書當日至履行合約訂明的責任為止
  8. The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing

    豬瘟病毒e2基因的真核表達：分別將csfv兩個代表株的e2基因克隆入畢赤酵母（ p . pastoris ）分泌型表達載體ppic9k中，酶切線型化后電穿孔導入p . pastotis進行整合，經g418篩選得到25個高拷貝轉化子，經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。
  9. By elisa analysis, inhibition of binding of clq with the c ! q receptors on u937 cells and competitive inhibition of binding of clq with aggregated immunoglobulin g b y selected phage clones, and dna sequencing, a number of similar, but not identical, sets of sequences of clq - binding clones were identified. the deduced amino acid sequences of selected 9 peptides are wyegpftlytwp, hwdpfslsayfp, ltqhnspffllp, tsnpfflwypqp, qtpfqlw, npfnwts, spfxlts, fltwldp and fstflyp. they show significant efficiency to inhibit the binding of clq with the clq receptors on u937 cells and / or aggregated immunoglobulin g, which suggest that the selected peptides contain the modeling epitopes of clq receptor to bind the collage - like region or igg to bind the head domain of clq

    然後，採用噬菌體肽庫技術，以c1q為釣餌蛋白，從12肽庫和環7肽庫中親和篩選能與c1q結合的噬菌體克隆，經elisa 、 u937細胞配體結合抑制試驗、 aigg競爭抑制試驗及dna測序，獲得了9個具c1q抑制活性克隆的dna序列，其相應的氨基酸序列為： wyegpftlytwp 、 hwdpfslsayfp 、 ltqhnspffllp 、 tsnpfflwypqp 、 qtpfqlw 、 npfnwts 、 spfxlts 、 fltwldp 、 fstflyp ，它們可能模擬c1qr和或igg的c1q結合表位並抑制c1q的活性。
  10. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  11. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
  12. After amplifying genomic sequences flanked with tn5gusa5 by tail pcr and dna sequencing, then doing blast with genomic sequence of xcc 8004, insertion sites of 10981 mutants were located precisely in xcc 8004 genome

    經tail - pcr擴增tn5gusa5旁側序列並測序，經與xcc8004基因組序列比對后獲得精確定位的tn5gusa5插入突變體10981個。
  13. The nucleic acids of the all influenza viruses conducted in the research were extracted from the viruses propagated in specific - pathogen - free chicken embryos. all of the eight segments were amplified by rt - pcr, and the purified pcr products were done cycle sequencing with specific primers, furthermore, the sequencing products were purified and run gel on abi prism 377 dna sequencing machine. the specific primers of the eight genes for pcr and cycle sequencing were designed using the ohgo ( 4. 0 version ) and genedoc ( 2. 3 version ) software

    首先將實驗用毒株在spf雞胚中增殖，提取核酸，然後應用oligo （ 4 . 0版本）和gendoc （ 2 . 3版本）軟體設計h9n2aiv所有8個基因片段特異的pcr引物及序列測定引物，通過rt - pcr方法擴增所有毒株的各個基因片段，純化後用特異引物進行測序反應，反應產物純化后在abiprism377dna序列分析儀上進行序列測定，然後應用wisconsinsequenceanalysispackage （ gcg ， 10 . 2版本） 、 phylogeneticanalysisusingparsimony （ paup ， 4 . 0版本）和treeview （ 1 . 5版本）軟體進行序列的數據編輯、序列翻譯、進化樹繪制和遺傳演化分析。
  14. After pcr checking and electro - transformation plasmid from c. elegans into dh5a, isolating plasmid from dhsct, digesting them with restriction enzymes and dna sequencing, six cdna fragments, which protein products can interact with rapgap, from cdna library were got

    將pcr擴增陽性及或lacz強陽性質粒電轉化入dhsa細菌，提取質粒后酶切、測序。發現有6個來源於celegqnscdna文庫的dna片段編碼的蛋白可以與ra帥ap相互作用，其中兩個為rapgap 。
  15. Dna sequencing of the appa gene showed an open reading frame of 1299 bp. the deduced appa phytase composed of 432 amino acids ( predicted molecular mass, 47. 06 kd ) also contained the reserved active - site motif rhgxrxp, which is shared by other phytases and acid phosphatases

    測序結果顯示appa基因閱讀框架為1299bp ，編碼432個氨基酸，編碼產物理論分子量為47 . 06kd ，同時它也具有其它植酸酶與酸性磷酸酶的活性保守基序rhgxrxp 。
  16. On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction

    本實驗在合成該蛋白基因上下游引物的基礎上，利用pcr技術，從人睪丸cdna庫中釣取目的基因，並克隆于pgem - t載體中，經序列測定證明與文獻報道基本一致，再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中，轉化宿主菌，經溫度誘導后，進行sds - page電泳分析，發現在約21kd位置上出現了一條明顯的蛋白帶，與預期相符。
  17. Four colonies of transformed e. coli dh5 a with clear hydroiyzing zone on the chitin agar were obtained. the gene fragment in these isolates was identified by the methods of plasmid processing. dna sequencing analysis showed that sequence homology between pcr fragment and chromosomal dna of b. subtilis from 2599451 to 2812870 was 85 %, and was 30 % between the fragment and the genes encoding for chitinase of bacillus ( including b. subtilis ) in genebank

    測序並序列比較結果表明該基因片段同已發表的枯草芽孢桿菌幾丁質酶和內切葡聚糖酶編碼幕因的克隆及重組芽抱桿菌的構建glyb一apre之間的同源性是最高的，為35 % ;同bacz 』了了ussp . bp23ce1b 、 b . p朋刀us內切葡聚糖酶和b . pol理vxap一1 ， 4一內切葡聚糖酶的編碼基因的同源性只有27 % 。
  18. After transformation, we have chosen the positive bacterium clones by bacterium colon pcr. with dna sequencing, we have made sure direction of dna sequence which inserted into the plasmid and have named sense and antisense reconstruction plas - mids respectively

    採用sanger雙脫氧末端終止法再進一步進行dna序列測定分析，檢測doc一1rcdna序列插人表達載體的方向，確定正、反義重組體。
  19. Thl2 gene was finally amplified by pcr with template genomic dna and cdna from roots, leaves, petals, stigmas and ovaries during bud stage in brassica oleracea l. ( 200110197 ) and brassica napus l. ( y578 - 2 ). the dna sequencing result shows that the genomic gene of thl2 is approximately 900 bp from brassica oleracea l. in contrast to that of 850 bp from brassica napus l.

    採用阿r和rtpcr技術，從甘藍和油菜基因組和柱頭總則a中擴增獲得了thlz基因，基因組中得到的基因大約為900hp和850hp ， cdna大約為477hp 。在thlz基因中首次發現了兩個內含于，並且甘藍和油菜thlz基因的內含于序列同源性低於ich ，並且大小也有差異。
  20. Results : designed and synthesized the coding gene of echistatin. and constructe its single copy expression vector, identify the vector by dna sequencing. but it could not express echistatin in e. coli. designed and synthesized the modified coding dna of echistatin, and constructe its two copy expression vector, identify the vector by dna sequencing, but it could not express echistatin in e. coli

    結果設計併合成了echistatin編碼基因dna序列，構建了echistatin單拷貝表達載體，經dna測序鑒定正確后， sds - page及western - blotting結果表明： echistatin在上述菌株中未獲得高效表達。
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