dntp 中文意思是什麼
dntp
解釋
三磷酸脫氧核苷-
2. 5 ul of 10 x reaction buffer, 1. 5 ul 25mm mgcl2, 0. 3 ul lomm dntp, 0. 5 ul taqdna polymerase ( 5 u / ul ), and lul ( = 20ng ) of primer were used in per reaction. each reaction was overlaid with one drop of paraffin oil. the initial denaturation step was used at 94 for 1 min 45 sec ; then denatured at 94 for 30 sec, annealed at 37 1 min, extended at iv b 72 for 2 min and repeated the cycle 45 times, at last, extended at 72 ' c for lomin
等（ 1995 ）利用rapd標記區分美國東部一雜交地帶的蟋蟀的兩個姐妹種， allonemobiusfasciatus和a . socius ，並於1998年使用rapd和異型酶標ic做出了這兩種蟋蟀的基因連鎖圖；國內田英芳、鄭哲民（ 20of ）首次將rapd技術運用於蟋蟀總科的分子系統學研究中，採用2種引物對7種蟋蟀進行了基困組dna多態性研究，並應用upg問a法構建樹狀圖，椎測系統發生關系 -
The routine method for inducing protein function evolution is imitating and speeding protein gene mutation. in this research - amylase gene was mutated in vitro by pcr with dntp in which deoxythymidine triphosphate ( dttp ) was partially replaced by 5 - bromo - 2 ' - deoxyuridine - 5 ' - triphosphate ( 5 - bdu ). the higher the concentration is, the stronger its inducing ability is in a certain range
本研究採用5 -溴脫氧尿苷三磷酸（ 5 - bdu ）部分取代脫氧胸苷三磷酸（ dttp ） ，在pcr的過程中對克隆的野油菜黃單胞菌的-澱粉酶基因進行了體外誘變，誘變結果表明： 5 - bdu濃度越高，誘變越強；濃度越低，誘變越弱。 -
The procedure of ddrt - pcr applicable for silver staining was optimized by adjusting the amount of several critical reagents, including total rna, anchor primer, arbitrary primer, cdna and dntp
通過調整ddrt - pcr中總rna 、錨定引物、隨機引物、 cdna和dntp等關鍵試劑的用量，優化了適用於銀染檢測的ddrt - pcr方法。 -
2. pcr products can be purified by ctab method, which removing the dntp and primers. the purified products can be used in the ligation reaction to pmd18 - t vector with high efficiency
2 、採用ctab進行pcr產物的純化，以去除pcr產物中的引物和dntp ，所得產物可用於與t載體連接反應，以提高連接效率。
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