dominant negative mutants 中文意思是什麼
dominant negative mutants
解釋
負突變物-
3 ) foa ( fluoroorotic acid ) treatment with the dominant negative mutants showed that the dominant negative effects of mutants were caused by the presence of the gpi17 vector, not by another mutation located somewhere in the genome and in a gene involved in the natural resistance against copper
3 ） foa （ 5 -氟-乳清酸）處理突變體細胞表明，得到的顯性負性突變體不是細胞中銅抗性基因突變的產物，其確為gpi17突變引起。 -
2 ) the growth curves for different cells show that overexpression of gpil7p has no lethal effect on the yeast cells and only that of the dominant negative mutants can cause cells not grow
2 ）生長曲線的測定表明，過表達gpi17p對正常酵母細胞不致死，而只有顯性負性突變體的過表達才會導致酵母細胞不能生長。 -
Dominant negative alleles of gpil7p were produced by random pcr mutagenesis of three conserved fragments of gpi17p : 1 ) having generated the plasmid library which contains 100000 plasmids harboring gpi17 mutants then screened from 8000 clones, we got 25 strong and 3 very strong dominant negative alleles
對gpi17p可能的三個保守區進行了pcr隨機誘變，並最終獲得顯性負性等位基因： 1 ）組建了gpi17p突變體質粒庫，其所含質粒數約為100000 。
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