enzymatic analysis 中文意思是什麼

In the first trial, combination of enzymatic digestion was used to prepare suspensions of spermatogenic cells from adult mouse testis, and then a modified discontinuous percoll gradient centrifugation method ( 15 %, 22 %, 30 %, 40 %, 50 %, 60 % ) was introduced to isolate spermatids from the cellular suspensions. the content of spermatids in each isolated fraction by percoll method was determined by morphology ( wright - giemsa stain ) and flow cytometry analysis, and the viability of spermatogenic cells was assessed by using eosin y exclusion test

在第一部分試驗中，首先利用連續3次組合酶消化成年小鼠睪丸制備睪丸細胞懸液，然後經6層非連續percoll梯度離心法（ 15 、 22 、 30 、 40 、 50和60 ）分離，通過形態學和流式細胞術鑒定南京醫科大學碩士學位論文各個percoll組分中精子細胞的含量，並以伊紅y排斥試驗測定細胞的存活率。

The nutrition analysis and debittering research on enzymatic hydrolysate of sheep bones

羊骨酶解液營養分析及脫苦研究

According to the chemical characteristics of organophosphorus pesticide and the principle of organophosphorus pesticide inhibit plants cholinesterase activity, based on determined enzymatic reaction conditions, the separation effect of different developing agent system on 11 organophosphorus pesticides by the means of thin - layer chromatography were researched, followed 10 qualitative analysis methods of organophosphorus pesticide residues were established

摘要根據有機磷農藥化學特性及其對植物膽堿酯酶抑制的原理，在確定酶促反應條件的基礎上，考察了不同展開體系對11種有機磷農藥的薄層層析分離效果，建立了10種有機磷農藥殘留的定性分析方法。

The recombinants were constructed by transforming ppic9 a - xynb into p. pastoris gs115. the assay results revealed that the xylanase gene xynb was overexpressed and secreted effectually in p. pastoris. in 3l fermentor the expression level of xylanase xynba exceeded 1200iu / ml and the expressed xylanase had normal bioactivity. the molecule weight of xynba was determined as about 31kd which is higher than 23kd of original enzyme xynb from streptomyces olivaceoviridis a1. xynbb was gotten by deglycasylation of xynba, whose molecule weight returned to 23kd. we comparised the enzymatic properties of xynba expressed in p. pastoris, xynbb deglycasylated from xynba and xynb produced from streptomyces olivaceoviridis al : there was little difference among the three enzymes on optimal ph, the optimal ph of xynb and xynba were both 5. 2, the optimal ph of xynbb was 5. 0 ; the optimal temperature of xynb and xynba were both 60 c, while the optimal temperature of xynbb was 50 ? ; because of glycosylation the thermal stability of xynba was better than xynb and xynbb ; the specific activity of xynba and xynbb were 883. 88iu / mg and 832. 5hu / mg respectively, which were both lower than 2814. 45iu / mg of xynb ; the km values of xynb and xynba were similar to each other which were 21. 56 ( g / kg ) and 20. 87 ( g / kg ), while the km value of xynbb was 27. 10 ( g / kg ) ; the fmax of xynba and xynbb were 4568umol / mg. min and 5329umol / mg. min respectively which were lower than 27623 umol / mg. min of xynb ; additionally all of the three enzymes did not display cellulase activity. they all had well resistance to pepsion and trypsin, and were not sensitive to metal iron, surface active agent and chelating agent. the analysis of different xylans enzymatic hydrolysate revealed : by xynba, that the main constitutions of enzymatic hydrolysate of birch wood xylans were xylotriose and xyloquaiose, which account for 68. 43 % and 16. 50 % respectively, additionally there was 11. 79 % of xylobiose ; the main constitutions of enzymatic hydrolysate of corncobs xylans were xylobiose and xylotriose, which account for 81. 78 % and 11. 55 %. the result indicated that this xylanase was a kind of 1, 4 - b - d - xylanohydrolase and was fit to used in industrial procession of xylooligosacc harides

進一步對xynba進行了脫糖基化處理得到xynbb ，其分子量恢復到23kd ，證明xynba是糖基化蛋白。通過對畢赤酵母重組表達的木聚糖酶xynba 、脫糖基化的木聚糖酶xynbb以及橄欖綠鏈黴菌a1所產原酶xynb之間酶學性質的比較發現：三種酶的最適ph差異不大， xynb和xynba均為5 . 2 ， xynbb為5 . 0 ； xynb和xynba的最適溫度均為60 ， xynbb降為50 ：在耐熱性上， xynba由於糖基化作用熱穩定性明顯高於未糖基化的xynb和xynbb ； xynba和xynbb的比活性分別為883 . 88iu mg和832 . 51iu mg ，明顯低於原酶的比活2814 . 45iu mg ； xynb和xynba的km值相當，分別為21 . 56 （ g kg ）和20 . 87 （ g kg ） ，而xynbb的km值較大為27 . 10 （ g kg ） ； xynba和xynbb的vmax相差不大，分別為4568 mol mg ? min和5329 mol mg ? min ，明顯低於xynb的27623 mol mg ? min此外三種酶均無纖維素酶活性，對胃蛋白酶和胰蛋白酶有很好的抗性，且對作用環境中的各種離子、表面活性劑、螯合劑不敏感。通過對不同木聚糖的酶解產物的糖份分析發現：以樺木木聚糖為底物時，酶解產物主要為木三糖和木四糖，含量分別為68 . 43和16 . 50 ，另外還含有11 . 79的木二糖；以玉米芯木聚糖為底物時，酶解產物主要為木二糖和木三糖，含量分別為81 . 78和11 . 55 。

Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質

With the procedures, the overall recovery of enzymatic activity reached 20 % and the specific activity for substrate hydantoin was about 4 u / mg protein. the purification factor was about 4. 7 folds with the purity about more than 95 % as estimated by sds - page analysis. d - hydantoinase gene from strain ss - ori was cloned to five different vectors to be five recombinant plasmids and in turn to transfer into five different e. coli strains, respectively

對其中產酶活性最高的一工程菌pexsec一hdt /雲coljblz ] （ de3 ）海因酶的表達條件進行了研究，目的蛋白的表達量約占總菌蛋白的20 % ，其酶活力為0 . 92u / ml ，約是原始菌株的d一海因酶表觀活性的4 . 6倍。

Analysis of honey - determination of ethanol content - enzymatic method

蜂蜜的分析.酒精含量的測定.酶催化法

For example, in 20 centuries 70 time, as the progress of molecular biology, be united in wedlock with project technology photograph, open up biology technology new field : gene recombines technology of cross of technology, pcr technology, dna and technology of protein alignment analysis, element to wait, the birth that promoted gene project, protein project, cell project, enzymatic project to wait and development

例如，在20世紀70年代，隨著分子生物學的進步，與工程技術相結合，開辟了生物技術新領域：基因重組技術、 pcr技術、 dna和蛋白質序列分析技術、分子雜交技術等，促進了基因工程、蛋白質工程、細胞工程、酶工程等的誕生和發展。

The result of the content analysis of lysophospholipids and glycerophospholipids indicated that 54. 3 % of phospholipids in the enzymatic degumming process translated into lysophospholipids, 45. 7 % translated into glycerophospholipids

對二者含量分析表明，酸法脫膠過程中54 . 3 %的磷脂轉變為溶血磷脂， 45 . 7 %的磷脂轉變為甘油酰磷脂。

Enzymatic synthesis and structure analysis of the monosubstituted derivative of - cyclomaltoheptaose

環糊精的酶促合成和結構分析

Enzymatic technology and analysis of enzymatic products of earthworm with subtilisin from bacillus subtilis as1

蚯蚓外源酶與內源酶酶解產物分析

In order to get some functional clues from their structures, the upstream regulation region of ndrgl gene and second structure of ndrg2 protein are performed bioinformatics analysis ; we found that there are several binding sequences of some diffirent transcription factors, their functions include regulating tissue - specific gene expression, regulating expression of genes related to growth and early development of cells, besides this, regulating expression of genes under some stimulated conditions, and so on. predict in protein fold classification shows that ndrg2 belongs to alpha / beta hydrolase fold family, and there are high similarity between ndrg2 and epoxide hydrolase from bacteria, this suggests that ndrg2 protein may has enzymatic functions associated with resisting the oxidative stress, maintaining the balance of cell redox potential, involving in the metabolism process of xenobiotics or intracellular toxic molecules

研究發現呷基因的調控區存在多種轉錄因子結合位點，功能主要涉及組織特異性表達調控，細胞生長發育相關基因的表達調控，刺激反應基因的表達調控等； ndrgz蛋白在結構上屬于a小水解酶類折疊，折疊分類預測表明ndrg2與其中的的細菌環氧化物水解酶的二級結構極為相似，提示ndrgz蛋白具有一定酶活性，可能參與細胞抗氧化應激反應，維持細， an ） armtbffiofbfochmilsyn ） mdafblechmrbfobo4第四軍醫大學碩士學位論文胞內氧還電勢平衡，參與內外源有毒物質的代謝等。

Testing of paper and board ; determination of starch content ; enzymatic analysis of native starch content

紙和紙板的檢驗.澱粉含量的測定.天然澱粉含量的酶分

The activity of the invertase of three high - sugar saccharomyces cerevisiae strains were investigated and their amino acid sequence were determined by the use of modern biotech including enzymatic activity determination, specific pcr, the cloning sequencing and bio - information analysis etc

摘要採用現代生物技術中的酶活測定、特異pcr 、克隆測序和生物信息分析等對比研究3株高糖酵母蔗糖轉換酶活性及其氨基酸序列。

Analysis of honey - determination of glycerol content - enzymatic method

蜂蜜的分析.丙三醇含量的測定.酶催化法